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RTCD1 Protein (AA 1-366) (His tag)
RTCD1
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 85 % by SDS - PAGE
SDS
N° du produit ABIN6387449
Détail du produit
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Antigène
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RTCD1
(RNA terminal Phosphate Cyclase Domain 1 (RTCD1))
Type de proteíne
Recombinant
Attributs du protein
AA 1-366
Origine
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Humain
Source
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Escherichia coli (E. coli)
Purification/Conjugué
Cette RTCD1 protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Séquence
MGSSHHHHHH SSGLVPRGSH MGSMAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL
Pureté
> 85 % by SDS - PAGE
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Stockage
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Format
Liquid
Concentration
0.25 mg/mL
Buffer
Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Détail du antigène
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Antigène
RTCD1
(RNA terminal Phosphate Cyclase Domain 1 (RTCD1))
Autre désignation
RTCA (RTCD1 Produits )
Synonymes
Dmel\\CG4061 Protein, EG:22E5.3 Protein, RTC1_DROME Protein, DKFZp469J2025 Protein, cb158 Protein, rtcd1 Protein, sb:cb158 Protein, wu:fc62c03 Protein, zgc:56339 Protein, RTCD1 Protein, RPC Protein, RTC1 Protein, 2310009A18Rik Protein, AI450277 Protein, Rtcd1 Protein, RNA 3'-terminal phosphate cyclase Protein, Rtca Protein, RTCA Protein, rtca Protein, rtca.L Protein
Sujet
RTCA catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP, (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A, (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. Recombinant human RTCA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Poids moléculaire
41.7 kDa (389aa) confirmed by MALDI-TOF
NCBI Accession
NP_003720
UniProt
O00442
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