ALT Protein (AA 1-496) (His tag)
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- Antigène Voir toutes ALT Protéines
- ALT (Alanine Aminotransferase (ALT))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 1-496
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette ALT protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS
- Pureté
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C
- Top Product
- Discover our top product ALT Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 2 mM DTT
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- ALT (Alanine Aminotransferase (ALT))
- Autre désignation
- GPT (ALT Produits)
- Synonymes
- AAT1 Protein, ALT1 Protein, GPT1 Protein, GPT Protein, Gpt1 Protein, An11g02620 Protein, AO090003000164 Protein, 233.t00009 Protein, 24.t00016 Protein, 1300007J06Rik Protein, 2310022B03Rik Protein, ALT Protein, Gpt-1 Protein, AU021132 Protein, Dpagt2 Protein, Gnpta Protein, gpt Protein, ALANINE AMINOTRANSFERAS Protein, AtAlaAT1 Protein, AtAlaATc Protein, T13M22.3 Protein, T13M22_3 Protein, alanine aminotransferas Protein, glutamic--pyruvic transaminase Protein, glutamic-pyruvate transaminase (alanine aminotransferase) Protein, alanine aminotransferase Protein, alanine aminotransferase 1 Protein, glutamic pyruvic transaminase, soluble Protein, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) Protein, alanine aminotransferase 2-like Protein, Alanine aminotransferase 1, mitochondrial Protein, GPT Protein, Gpt Protein, Tb927.1.3950 Protein, ANI_1_368094 Protein, AOR_1_284154 Protein, ALAT_1 Protein, ALAAT1 Protein, EHI_159710 Protein, EHI_096750 Protein, LOC5572541 Protein, ALAT Protein, AAT2 Protein, Dpagt1 Protein, LOC100537633 Protein, AlaAT1 Protein
- Sujet
- GPT, also known as alanine aminotransferases (ALT1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Recombinant human GPT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Poids moléculaire
- 56.8 kDa (516aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_005300
- UniProt
- P24298
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