GDA Protein (AA 1-253) (His tag)
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- Antigène Voir toutes GDA Protéines
- GDA (Guanine Deaminase (GDA))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 1-253
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette GDA protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MGSMCAAQMP PLAHIFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE ASQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHASQ YSFAGSSIDL PLLEWLTKYT FPAEHRFQNI DFAEEVYTRV VRRTLKNGTT TACYFATIHT DSSLLLADIT DKFGQRAFVG KVCMDLNDTF PEYKETTEES IKETERFVSE MLQKNYSRVK PIVTPRFSLS CSETLMGELG NIAKTRDLHI QSHISENRDE VEAVKNLYPS YKNYTSVYDK NNLLTNKTVM AHGCYLSAEE LNVFHERGAS IAHCPNSNLS LSSGFLNVLE VLKHEVKIGL GTDVAGGYSY SMLDAIRRAV MVSNILLINK VNEKSLTLKE VFRLATLGGS QALGLDGEIG NFEVGKEFDA ILINPKASDS PIDLFYGDFF GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV
- Pureté
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is >2,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
- Top Product
- Discover our top product GDA Protéine
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Guanine Deaminase (GDA) (AA 1-454) protein (GST tag)
GDA Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
Guanine Deaminase (GDA) (AA 1-454) protein (His tag)GDA Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Guanine Deaminase (GDA) (AA 1-454) protein (Strep Tag)Crystallography grade GDA Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Guanine Deaminase (GDA) (AA 1-454) protein (Strep Tag)Crystallography grade GDA Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Guanine Deaminase (GDA) (AA 1-454) (Active) protein (His tag)GDA Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS Active
Guanine Deaminase (GDA) (AA 1-454) (Active) proteinGDA Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS Active
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- GDA (Guanine Deaminase (GDA))
- Autre désignation
- GDA (GDA Produits)
- Synonymes
- CYPIN Protein, GUANASE Protein, NEDASIN Protein, AU015411 Protein, AW047581 Protein, Cypin Protein, MGC81835 Protein, zgc:112282 Protein, AGR_C_4212 Protein, DDBDRAFT_0169373 Protein, DDBDRAFT_0230211 Protein, DDB_0169373 Protein, DDB_0230211 Protein, guanine deaminase Protein, guanine deaminase L homeolog Protein, short-chain dehydrogenase/reductase sdr Protein, GDA Protein, Gda Protein, gda.L Protein, gda Protein, SPO2956 Protein, Gdia_3192 Protein, Mchl_2046 Protein, Mnod_4655 Protein, guaD Protein, Dd586_1771 Protein, Kvar_2512 Protein, Mrub_1462 Protein, BC1002_2263 Protein, Cseg_1079 Protein, Mesil_1795 Protein, Trad_2850 Protein, Mesop_0346 Protein, gDA Protein, ML5_2802 Protein
- Sujet
- GDA is an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Poids moléculaire
- 53kDa (477aa)
- NCBI Accession
- NP_004284
- UniProt
- Q9Y2T3
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