MDH2 Protein (AA 25-338) (His tag)

N° du produit ABIN6388154

Détail du produit

Antigène: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 25-338

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette MDH2 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK

Pureté: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In Phosphate buffer saline (  pH 7.4) containing 20 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Autre désignation: Mdh2 (MDH2 Produits)

Synonymes: Mdh2b Protein, m-mdh Protein, mdh2 Protein, mor1 Protein, MDH-2 Protein, Mdh Protein, wu:fj48c08 Protein, wu:fj55d06 Protein, zgc:64133 Protein, M-MDH Protein, MDH Protein, MGC:3559 Protein, MOR1 Protein, MMDH Protein, Mdh-2 Protein, Mor-1 Protein, Mor1 Protein, malate dehydrogenase 2 S homeolog Protein, malate dehydrogenase 2 Protein, Malate dehydrogenase 2 Protein, malate dehydrogenase Protein, malate dehydrogenase 2, NAD (mitochondrial) Protein, mdh2.S Protein, MDH2 Protein, Mdh2 Protein, MDH4 Protein, mdh2 Protein

Sujet: Mdh2, also known as Malate dehydrogenase 2. Mdh2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, Mdh2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant mouse Mdh2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 35.4 kDa (335aa) confirmed by MALDI-TOF

NCBI Accession: NP_032643

UniProt: P08249