TUBB Protein (GST tag)

N° du produit ABIN6700746

N° du produit (Fournisseur): 009-001-u24s

Protéine Recombinant TUBB exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour TUBB Protein (GST tag) (ABIN6700746)

Antigène: TUBB (Tubulin, beta (TUBB))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: >85%

Détail du produit

Purification/Conjugué: Cette TUBB protéine est marqué à la GST tag.

N° du produit (Fournisseur): 009-001-u24s

Fournisseur: Rockland

Fonction: Tubulin beta (TUBB1) recombinant protein-GST fusion protein

Purification: Recombinant full-length human TUBB1 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Information d'application

Indications d'application:

Application Note: TUBB1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. TUBB1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~76 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: TUBB1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Stock: -80 °C

Stockage commentaire: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: TUBB (Tubulin, beta (TUBB))

Autre désignation: TUBB

Sujet:

Synonyms: M40, TUBB, TUBB5, MGC16435, MGC117247, OK/SW-cl.56, Tubulin beta chain, Tubulin beta-5 chain

Background: TUBB1 or tubulin-beta 1 protein is a major constituent of microtubules. TUBB1 interaction with microtubule-associated proteins (MAPs) such as tau is fundamental for microtubule structure and function (1). Previous work suggested that the "microtubule binding domain" of tau (composed of three or four imperfect 18-amino acid repeats, separated by 13- or 14-amino acid inter-repeat regions) can bind to the C-terminal ends of both alpha and beta tubulin monomers. Studies revealed that TUBB1 is the target of various antitubulin agents used in the treatment of cancer. Subsequent studies have also concluded that TUBB1 mutations in clinical samples are rare, and unlikely to contribute to drug resistance (2). TUBB1 Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Cardiovascular Disease, and Neurobiology research.

NCBI Accession: NM_178014

Pathways: Dynamique des Microtubules, M Phase