ACE2 Protein (Fc Tag,AVI tag,Biotin)

N° du produit ABIN6952458

Détail du produit

Antigène: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette ACE2 protéine est marqué à la Fc Tag,AVI tag,Biotin.

Séquence: AA 18-740

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Attributs du produit: Biotinylated Human ACE2, Fc,Avitag is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1). Predicted N-terminus: Gln 18
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: The protein has a calculated MW of 111.7 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: The product MUST be stored at -70°C or lower upon receipt
-70°C for 3 months under sterile conditions.

Référence

Dogan, Kozhaya, Placek, Gunter, Yigit, Hardy, Plassmeyer, Coatney, Lillard, Bukhari, Kleinberg, Hayes, Arditi, Klapper, Merin, Liang, Gupta, Alpan, Unutmaz: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." dans: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).

Chouchane, Grivel, Farag, Pavlovski, Maacha, Sathappan, Al-Romaihi, Abuaqel, Ata, Ismail Chouchane, Remadi, Halabi, Rafii, Al-Thani, Marr, Subramanian, Shan: "Dromedary camels as a natural source of neutralizing nanobodies against SARS-CoV-2." dans: JCI insight, (2021) (PubMed).

Détail du antigène

Antigène: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Autre désignation: ACE2 (ACE2 Produits)

Synonymes: ACE1 Protein, CD143 Protein, DCP Protein, DCP1 Protein, ICH Protein, MVCD3 Protein, ACEH Protein, 2010305L05Rik Protein, zgc:92514 Protein, angiotensin I converting enzyme Protein, angiotensin I converting enzyme 2 Protein, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 Protein, ACE Protein, ACE2 Protein, Ace2 Protein, ace2 Protein

Sujet: Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

Poids moléculaire: 111.7 kDa

NCBI Accession: NP_068576

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour