SARS-CoV-2 NSP1 Protein (His tag)

N° du produit ABIN6952638

Détail du produit

Antigène: SARS-CoV-2 NSP1 (NSP1) (SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1))

Type de proteíne: Recombinant

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette SARS-CoV-2 NSP1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Fonction: SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag

Séquence: AA 1-180

Attributs du produit: SARS-CoV-2 NSP1, His Tag is expressed from E.coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1). Predicted N-terminus: Met
This protein carries a polyhistidine tag at the C-terminus.

Pureté: >90 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, Arginine, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Détail du antigène

Antigène: SARS-CoV-2 NSP1 (NSP1) (SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1))

Autre désignation: SARS-CoV-2 Host Translation Inhibitor Nsp1 (NSP1 Produits)

Synonymes: pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein

Classe de substances: Viral Protein

Sujet: The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.

Poids moléculaire: 21.7 kDa

NCBI Accession: YP_009725297