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SARS-CoV-2 Spike S1 Protein (His tag,Fc Tag)

Origine: SARS Coronavirus-2 (SARS-CoV-2) Hôte: HEK-293 Cells Recombinant > 90 % by SDS-PAGE. SDS, Func Active
N° du produit ABIN6952732
  • Antigène Voir toutes SARS-CoV-2 Spike S1 Protéines
    SARS-CoV-2 Spike S1
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Origine
    • 94
    • 9
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 86
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification/Conjugué
    Cette SARS-CoV-2 Spike S1 protéine est marqué à la His tag,Fc Tag.
    Application
    SDS-PAGE (SDS), Functional Studies (Func)
    Séquence
    VSSQCVNLTT RTQLPPAYTN SFTRGVYYPD KVFRSSVLHS TQDLFLPFFS NVTWFHAIHV SGTNGTKRFD NPVLPFNDGV YFASTEKSNI IRGWIFGTTL DSKTQSLLIV NNATNVVIKV CEFQFCNDPF LGVYYHKNNK SWMESEFRVY SSANNCTFEY VSQPFLMDLE GKQGNFKNLR EFVFKNIDGY FKIYSKHTPI NLVRDLPQGF SALEPLVDLP IGINITRFQT LLALHRSYLT PGDSSSGWTA GAAAYYVGYL QPRTFLLKYN ENGTITDAVD CALDPLSETK CTLKSFTVEK GIYQTSNFRV QPTESIVRFP NITNLCPFGE VFNATRFASV YAWNRKRISN CVADYSVLYN SASFSTFKCY GVSPTKLNDL CFTNVYADSF VIRGDEVRQI APGQTGKIAD YNYKLPDDFT GCVIAWNSNN LDSKVGGNYN YLYRLFRKSN LKPFERDIST EIYQAGSTPC NGVEGFNCYF PLQSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGPKKS TNLVKNKCVN FNFNGLTGTG VLTESNKKFL PFQQFGRDIA DTTDAVRDPQ TLEILDITPC SFGGVSVITP GTNTSNQVAV LYQDVNCTEV PVAIHADQLT PTWRVYSTGS NVFQTRAGCL IGAEHVNNSY ECDIPIGAGI CASYQTQTNS PR
    Specificité
    Val11-Arg682
    Attributs du produit
    Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6xHis tag at the C-terminus.
    Pureté
    > 90 % by SDS-PAGE.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    < 0.1 EU/μg of the protein by LAL method.
    Biological Activity Comment
    1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Spike S1, the EC50 of SARS-COV-2 Spike S1 is 6.79 ng/mL.< /br>2.Immobilized Human ACE2 on COOH Chip can bind SARS-COV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay (Nicoya OpenSPR).
    Top Product
    Discover our top product SARS-CoV-2 Spike S1 Protéine
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
    Concentration
    1 mg/mL
    Buffer
    Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    4 °C,-20 °C,-80 °C
    Stockage commentaire
    Store the lyophilized protein at -20°C to -80 °C for long term.|After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. |For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
  • Antigène
    SARS-CoV-2 Spike S1
    Abstract
    SARS-CoV-2 Spike S1 Produits
    Synonymes
    E2 Protein, Surface Glycoprotein Protein, S Protein
    Classe de substances
    Viral Protein
    Sujet

    Description: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

    Name: Envelope,SARS-CoV-2 Spike RBD (N501Y),Spike,Spike ECD,Spike RBD,Spike S1,Spike S2,Spike S2 ECD,S1-RBD protein,NCP-CoV RBD Protein,novel coronavirus RBD Protein,2019-nCoV RBD Protein,S glycoprotein Subunit1 RBD Protein

    ID gène
    43740568
    UniProt
    P0DTC2
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