SARS-CoV-2 Spike Protein (D614G, Super Stable Trimer) (His tag,AVI tag,Biotin)

N° du produit ABIN6953300

Détail du produit

Antigène: SARS-CoV-2 Spike

Type de proteíne: Recombinant

Attributs du protein: D614G, Super Stable Trimer

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV-2 Spike protéine est marqué à la His tag,AVI tag,Biotin.

Application: ELISA

Fonction: Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (MALS verified)

Séquence: AA 16-1213

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Attributs du produit: Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1(D614G) and D614G mutation, which has become increasingly common in SARS-CoV-2 viruses from around the world .
The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus.
Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
It is the biotinlynated form of SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified) SPN-C52H3).

Pureté: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS

Stock: -20 °C,-80 °C

Stockage commentaire: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state
70°C for 3 months under sterile conditions after reconstitution.

Date de péremption: 12 months

Détail du antigène

Antigène: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Produits

Synonymes: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Classe de substances: Viral Protein

Sujet: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 139.7 kDa