SARS-CoV-2 Spike Protein (D614G, Super Stable Trimer) (His tag)

N° du produit ABIN6953301

Détail du produit

Antigène: SARS-CoV-2 Spike

Type de proteíne: Recombinant

Attributs du protein: D614G, Super Stable Trimer

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV-2 Spike protéine est marqué à la His tag.

Application: ELISA

Fonction: SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified)

Attributs du produit: SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1) and D614G mutation, which has become increasingly common in SARS-CoV-2 viruses from around the world . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Pureté: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS

Stock: -20 °C,-80 °C

Stockage commentaire: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state
70°C for 3 months under sterile conditions after reconstitution.

Date de péremption: 12 months

Détail du antigène

Antigène: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Produits

Synonymes: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Classe de substances: Viral Protein

Sujet: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 138.0 kDa