SARS-CoV-2 Spike Protein (B.1.1.529 - Omicron, RBD) (His tag)

N° du produit ABIN7041436

Détail du produit

Antigène: SARS-CoV-2 Spike

Type de proteíne: Recombinant

Attributs du protein: B.1.1.529 - Omicron, RBD

Origine: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV-2 Spike protéine est marqué à la His tag.

Application: ELISA, SDS-PAGE (SDS)

Fonction: SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (MALS verified)

Attributs du produit: SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: B.1.1.529; GISAID clade: GR/484A; Nextstrain clade: 21K). Predicted N-terminus: Arg 319

Pureté: > 95% as determined by SDS-PAGE. > 95% as determined by SEC-MALS.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C/-80 °C

Stockage commentaire: For long term storage, the product should be stored at lyophilized state at -20°C or lower.This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 months under sterile conditions after reconstitution.

Date de péremption: 12 monts

Référence

Vogt, Augusto, Martina, Chang, Nasrallah, Speiser, Vogel, Bachmann, Mohsen: "Increased Receptor Affinity and Reduced Recognition by Specific Antibodies Contribute to Immune Escape of SARS-CoV-2 Variant Omicron." dans: Vaccines, Vol. 10, Issue 5, (2022) (PubMed).

Détail du antigène

Antigène: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Produits

Synonymes: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Classe de substances: Viral Protein

Sujet: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 26.8 kDa