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HTRA1 Protein (full length) (rho-1D4 tag)

Crystallography grade HTRA1 Origine: CHO cells Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys, Func
N° du produit ABIN7126064
  • Antigène Voir toutes HTRA1 Protéines
    HTRA1 (HtrA Serine Peptidase 1 (HTRA1))
    Type de proteíne
    Recombinant
    Attributs du protein
    full length
    Origine
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    CHO cells
    Source
    • 6
    • 2
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette HTRA1 protéine est marqué à la rho-1D4 tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), Functional Studies (Func)
    Séquence
    MASTDNLCGH VIPEAGKTEL SARPGPQRNG AVLVGLLQCG RAHISSQTLP HPAVIAEGKL PVLLLGRSSE LRPGEFVVAI GSPFSLQNTV TTGIVSTTQR GGKELGLRNS DMDYIQTDAI INDGEVIGIN TLKVTAGISF AIPSDKIKKF LTESHDRQAK GKAVTKKKYI GIRMMSLTSS SKAKELKDRH RDFPDVLSGA YIIEVIPDTP AEAGGLKEND VIISINGQSV VTANDVSDVI KKENTLNMVV RRGNEDIMLT VIPEEIDP
    Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • CHO Serine protease HTRA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
    Classe de qualité
    Crystallography grade
    Biological Activity Comment
    Protein has not been tested for activity yet.
    Top Product
    Discover our top product HTRA1 Protéine
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    HTRA1 (HtrA Serine Peptidase 1 (HTRA1))
    Autre désignation
    Serine protease HTRA1 (HTRA1 Produits)
    Synonymes
    htra1 Protein, zgc:92029 Protein, zgc:172061 Protein, l56 Protein, htra Protein, Xhtra1 Protein, prss11 Protein, MGC145723 Protein, ARMD7 Protein, CARASIL Protein, HtrA Protein, L56 Protein, ORF480 Protein, PRSS11 Protein, AI429470 Protein, HTRA Protein, Prss11 Protein, RSPP11 Protein, HTRA1 Protein, HtrA serine peptidase 1a Protein, HtrA serine peptidase 1 Protein, HtrA serine peptidase 1b Protein, Serine protease HTRA1 Protein, HtrA serine peptidase 1 S homeolog Protein, htra1a Protein, HTRA1 Protein, htra1b Protein, htra1 Protein, Htra1 Protein, htra1.S Protein
    UniProt
    G3IBF4
    Pathways
    Growth Factor Binding
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