Calmodulin 1 Protein (Calm1) (full length) (rho-1D4 tag)

N° du produit ABIN7126080

Détail du produit

Antigène: Calmodulin 1 (Calm1)

Type de proteíne: Recombinant

Attributs du protein: full length

Origine: CHO cells

Source: Cellules d'insectes

Purification/Conjugué: Cette Calmodulin 1 protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)

Séquence: MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO CALM1 (Calmodulin 2) (Caltractin-like protein) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Classe de qualité: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Calmodulin 1 (Calm1)

Autre désignation: CALM1 (Calmodulin 2) (Calm1 Produits)

Synonymes: AI256814 Protein, AI327027 Protein, AI461935 Protein, AL024000 Protein, CaM Protein, Calm Protein, CaMI Protein, CALML2 Protein, CAMI Protein, CPVT4 Protein, DD132 Protein, LQT14 Protein, PHKD Protein, caM Protein, calm1 Protein, calm2-a Protein, calm2-b Protein, calm2a Protein, calml2 Protein, cami Protein, dd132 Protein, 3909 Protein, CAM Protein, CG8472 Protein, Cal Protein, Cal49A Protein, CalA Protein, Cam1 Protein, D-CaM Protein, DmCaM Protein, DmCalm Protein, Dmel\CG8472 Protein, anon-EST:Posey59 Protein, cam Protein, dCaM Protein, l(2)03909 Protein, l(2)3909 Protein, lincRNA.S2634 Protein, calmodulin 1 Protein, calmodulin 2 Protein, calmodulin 1 (phosphorylase kinase, delta) L homeolog Protein, Calmodulin Protein, Calm1 Protein, Calm2 Protein, CALM1 Protein, calm1.L Protein, Cam Protein

UniProt: A0A061HUH1

Pathways: cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Phototransduction, BCR Signaling