DLL4 Protein (AA 27-524) (hFc Tag)

N° du produit ABIN7274485

Cette protéine Recombinant DLL4 est exprimée dans HEK-293 Cells.

Aperçu rapide pour DLL4 Protein (AA 27-524) (hFc Tag) (ABIN7274485)

Antigène: DLL4 (delta-Like 4 (DLL4))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Pureté: > 95 % as determined by Bis-Tris PAGE.,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 27-524

Purification/Conjugué: Cette DLL4 protéine est marqué à la hFc Tag.

Fonction: Human DLL4 Protein

Séquence: Ser27-Pro524

Attributs du produit: Recombinant Human DLL4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ser27-Pro524.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 uM as determined in SPR assay (Biacore T200).,Immobilized Human Notch 1, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3 ng/ml determined by ELISA (QC Test).,Immobilized Human DLL4, hFc Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 23.5 ng/ml determined by ELISA.,Human DLL4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human DLL4 is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: DLL4 (delta-Like 4 (DLL4))

Autre désignation: DLL4

Sujet: Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.

Poids moléculaire: 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on Bis-Tris PAGE result.

UniProt: Q9NR61

Pathways: Signalisation Notch