TNFRSF4
(Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
Type de proteíne
Recombinant
Attributs du protein
AA 20-211
Origine
Souris
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFRSF4 protéine est marqué à la His-Avi Tag,Biotin.
Fonction
Biotinylated Mouse OX40/TNFRSF4/CD134 Protein
Séquence
Val20-Pro211
Attributs du produit
Recombinant Biotinylated Mouse OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val20-Pro211.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse OX40 Ligand, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse OX40, His Tag with the EC50 of 4.3μg/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFRSF4
(Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation, its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
Poids moléculaire
24.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.