RNF152 Protein (AA 1-203) (His tag)

N° du produit ABIN7551517

Cette protéine Recombinant RNF152 est produite dans HEK-293 Cells.

Aperçu rapide pour RNF152 Protein (AA 1-203) (His tag) (ABIN7551517)

Antigène: RNF152 (Ring Finger Protein 152 (RNF152))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Classe de qualité: custom-made

Détail du produit

Attributs du protein: AA 1-203

Purification/Conjugué: Cette RNF152 protéine est marqué à la His tag.

Fonction: Predefined custom protein recombinant RNF152 Protein expressed in mammalian cells.

Séquence: METLSQDSLL ECQICFNYYS PRRRPKLLDC KHTCCSVCLQ QMRTSQKDVR CPWCRGVTKL PPGFSVSQLP DDPEVLAVIA IPHTSEHTPV FIKLPSNGCY MLPLPISKER ALLPGDMGCR LLPGSQQKSV TVVTIPAEQQ PLQGGAPQEA VEEEQDRRGV VKSSTWSGVC TVILVACVLV FLLGIVLHNM SCISKRFTVI SCG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificité: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Attributs du produit: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Information d'application

Indications d'application: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: 12 months

Détail du antigène

Antigène: RNF152 (Ring Finger Protein 152 (RNF152))

Autre désignation: RNF152

Sujet: E3 ubiquitin-protein ligase RNF152 (EC 2.3.2.27) (RING finger protein 152) (RING-type E3 ubiquitin transferase RNF152),FUNCTION: E3 ubiquitin-protein ligase that acts as a negative regulator of mTORC1 signaling by mediating ubiquitination of RagA/RRAGA and RHEB (PubMed:25936802, PubMed:30514904). Catalyzes 'Lys-63'-linked polyubiquitination of RagA/RRAGA in response to amino acid starvation, thereby regulating mTORC1 signaling (PubMed:25936802). Also mediates monoubiquitination of RHEB, promoting its association with the TSC-TBC complex and subsequent inhibition (PubMed:30514904). Also mediates 'Lys-48'-linked polyubiquitination of target proteins and their subsequent targeting to the proteasome for degradation (PubMed:21203937). Induces apoptosis when overexpressed (PubMed:21203937). {ECO:0000269|PubMed:21203937, ECO:0000269|PubMed:25936802, ECO:0000269|PubMed:30514904}.

Poids moléculaire: 22.4 kDa

UniProt: Q8N8N0

AlphaFold: Q8N8N0