RNF146 Protein (AA 1-359) (His tag)

N° du produit ABIN7564967

Cette protéine Recombinant RNF146 est exprimée dans HEK-293 Cells.

Aperçu rapide pour RNF146 Protein (AA 1-359) (His tag) (ABIN7564967)

Antigène: RNF146 (Ring Finger Protein 146 (RNF146))

Type de proteíne: Recombinant

Origine: Souris

Source: HEK-293 Cells

Pureté: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Classe de qualité: custom-made

Détail du produit

Attributs du protein: AA 1-359

Purification/Conjugué: Cette RNF146 protéine est marqué à la His tag.

Fonction: Predefined custom protein recombinant Rnf146 Protein expressed in mammalian cells.

Séquence: MEMAGCGEID HSINMLPTNK KANESCSNTA PSLTVPECAI CLQTCVHPVS LPCKHVFCYL CVKGASWLGK RCALCRQEIP EDFLDKPTLL SPEELKAASR GNGEYAWYYE GRNGWWQYDE RTSRELEDAF SKGKKNTEML IAGFLYVADL ENMVQYRRNE HGRRRKIKRD IIDIPKKGVA GLRLDCDTNT VNLARESSAD GADSGSAQTG ASVQLAVPSS TRPLTSVDGQ LTSPVTPSPD AGISLEDSFA HLQLSGDSIA ERSHRGEGEE DHESPSSGRV PDTSVEETES DASSDSEDAP VVVAQHSLTQ QRPLVPNGNQ TVADQSDRSG TDRSVAGGGT MSVNVRSRRP DGQCTVTEV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificité: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Attributs du produit: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Information d'application

Indications d'application: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: 12 months

Détail du antigène

Antigène: RNF146 (Ring Finger Protein 146 (RNF146))

Autre désignation: Rnf146

Sujet: E3 ubiquitin-protein ligase RNF146 (EC 2.3.2.27) (Iduna) (RING finger protein 146) (RING-type E3 ubiquitin transferase RNF146),FUNCTION: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein (By similarity). Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos (PubMed:21602803, PubMed:21825151). Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner (PubMed:21602803, PubMed:21825151). Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest (PubMed:21602803, PubMed:21825151). Promotes cell survival after gamma-irradiation. Facilitates DNA repair (By similarity). {ECO:0000250|UniProtKB:Q9NTX7, ECO:0000269|PubMed:21602803, ECO:0000269|PubMed:21825151}.

Poids moléculaire: 38.9 kDa

UniProt: Q9CZW6

AlphaFold: Q9CZW6