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TNF alpha Protein (full length)

TNF alpha Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 98 % pure Active
N° du produit ABIN935842
  • Antigène Voir toutes TNF alpha Protéines
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Attributs du protein
    full length
    Origine
    • 43
    • 24
    • 12
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 61
    • 28
    • 23
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Attributs du produit
    Purified recombinant Human TNF alpha protein
    Expression System: E.coli
    Bioactivity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is
    Purification
    Proprietary chromatographic technique
    Pureté
    > 98 % pure
    Top Product
    Discover our top product TNF alpha Protéine
  • Indications d'application
    Each Investigator should determine their own optimal working dilution for specific applications.
    Procédure de l'essai

    Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
    2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute in water to a concentration of 0.1 - 1.0 g/mL.
    Buffer
    Lyophilized from 20 mM PBS, pH 7.2, with 10 mM NaCl.
    Conseil sur la manipulation
    Avoid repeated freeze/thaw cycles.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
  • Spatz, Eibl, Hink, Wolf, Fischer, Mayr, Schernthaner, Eibl: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." dans: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).

  • Antigène
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Autre désignation
    TNF alpha (TNF alpha Produits)
    Synonymes
    DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein
    Sujet
    Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
    Alternative Names: Tumor Necrosis Factor protein, TNFa protein, Cytotoxin protein, DIF protein, TNFSF2 protein, TNF-alpha protein, TNF-a protein, Differentiation-inducing factor protein, Necrosin protein, Cachectin protein
    Poids moléculaire
    17,483 Da
    Pathways
    Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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