anti-Chien ATP Synthase Subunit beta anticorps pour BioImaging

Recommended ATP Synthase Subunit beta Antibody (fourni par: Connectez-vous pour afficher )

Antigène
ATP Synthase Subunit beta (AtpB) Anticorps
  • DDBDRAFT_0190669
  • DDBDRAFT_0233951
  • DDB_0190669
  • DDB_0233951
  • ATP
  • ATP-syn-B
  • ATP-synbeta
  • ATPB
  • ATPase beta
  • ATPasebeta
  • ATPsyn b
  • ATPsyn-&bgr
  • ATPsyn-b
  • CG11154
  • Dmel\\CG11154
  • beta-ATPase
  • GLEAN_15322
  • ATP synthase beta chain, mitochondrial
  • ATP synthase, beta subunit
  • ATP synthase subunit beta, mitochondrial
  • ATP synthase-beta
  • ATP synthase subunit b
  • ATP synthase archaeal, B subunit
  • atp5b
  • ATPsynbeta
  • LOC100401662
  • ATPsyn-beta
  • Atpsyn-beta
  • LOC100635763
  • Selsp_1843
  • Thena_0594
  • Halhy_3257
  • MZHIL_RS07425
Épitope
AA 428-539
4
1
1
1
1
Reactivité
Poulet, Chien, Humain, Souris, Rat (Rattus)
4
4
3
2
Hôte
Souris
5
4
Clonalité
Monoclonal
Conjugué
Inconjugué
1
1
1
Application
BioImaging (BI), Western Blotting (WB)
5
4
2
2
2
2
1
1
1
Options
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N° du produit ABIN968859
Produit non disponible pour cette région.

General

Antigène ATP Synthase Subunit beta (AtpB) Anticorps
Épitope AA 428-539
(4), (1), (1), (1), (1)
Reactivité Poulet, Chien, Humain, Souris, Rat (Rattus)
(4), (4), (3), (2)
Hôte Souris
(5), (4)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Inconjugué
(1), (1), (1)
Application BioImaging (BI), Western Blotting (WB)
(5), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 3 références disponible
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Détail du produit

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 Réactivité croisée Poulet, Chien, Souris, Rat (Rattus)
Attributs du produit 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Triton is a trademark of the Dow Chemical Company.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogène Human ATP Synthase beta aa. 428-539
Clone 10-ATP
Isotype IgG1

Détail du antigène

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Antigène
Autre désignation ATP Synthase beta (AtpB Antibody Extrait)
Sujet ATP synthase is a large enzyme complex that uses an electrochemical H+ or Na+ gradient to synthesize ATP from ADP and Pi, providing the organism with the ATP needed for energy. The complex consists of two major units, F0 and F1. F0 is embedded in the inner membrane of the mitochondria and, due to its hydrophobic nature, translocates protons across this membrane. F1 is the catalytic portion in the matrix region of the mitochondria and is comprised of alpha, beta, gamma, delta, and epsilon subunits at a 3:3:1:1:1 ratio. The beta subunit is synthesized in the nuclear genome,transported to the mitochondria, and assembled with the other subunits. It is encoded by a single copy gene, is ubiquitously expressed and highly conserved among species. ATP synthase beta contains an Ets domain binding site, which is a main site for promoter activity. Ets proteins contain domains that are involved in transcriptional activation, protein-protein interactions, and intramolecular repression of DNA binding. This site acts as a target for transcriptional control by the Ets family of transcription factors. Thus, ATP synthase beta is involved in the synthesis of ATP and is controlled in part by ETS family proteins.
Poids moléculaire 55 kDa
Pathways Proton Transport, Ribonucleoside Biosynthetic Process

Information d'application

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Indications d'application Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Commentaires

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Stockage

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Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store undiluted at -20°C.

Référence

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Produit citée dans:

Villena, Martin, Viñas, Cormand, Iglesias, Mampel, Giralt, Villarroya: "ETS transcription factors regulate the expression of the gene for the human mitochondrial ATP synthase beta-subunit." dans: The Journal of biological chemistry, Vol. 269, Issue 51, pp. 32649-54, 1995

Lee, Garboczi, Thomas, Pedersen: "Mitochondrial ATP synthase. cDNA cloning, amino acid sequence, overexpression, and properties of the rat liver alpha subunit." dans: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4664-9, 1990

Ohta, Tomura, Matsuda, Kagawa: "Gene structure of the human mitochondrial adenosine triphosphate synthase beta subunit." dans: The Journal of biological chemistry, Vol. 263, Issue 23, pp. 11257-62, 1988

Images

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Images (Fournisseur)
Western Blotting (WB) image for anti-ATP Synthase Subunit beta (AtpB) (AA 428-539) antibody (ABIN968859) Western blot analysis of ATP Synthase beta on a Jurkat cell lysate. Lane 1: 1:10,000,...
Immunofluorescence (IF) image for anti-ATP Synthase Subunit beta (AtpB) (AA 428-539) antibody (ABIN968859) Immunofluorescent staining of A549 (ATCC CCL-185) cells. Cells were seeded in a 96 we...
Western Blotting (WB) image for anti-ATP Synthase Subunit beta (AtpB) (AA 428-539) antibody (ABIN968859) anti-ATP Synthase Subunit beta (AtpB) (AA 428-539) antibody (Image 3)