anti-Rat (Rattus) TACR1 anticorps pour Immunohistochemistry (Frozen Sections)

Recommended TACR1 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Tachykinin Receptor 1 (TACR1) Anticorps
  • NK1R
  • NKIR
  • SPR
  • TAC1R
  • Nk1r
  • Spr
  • Tac1r
  • nk1R
  • Tacr1
  • ASPR
  • tachykinin receptor 1
  • TACR1
  • Tacr1
Épitope
C-Term
23
18
15
14
11
8
7
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivité
Cobaye, Humain, Souris, Rat (Rattus)
150
71
64
10
10
6
5
5
4
4
2
2
1
1
1
1
Hôte
Lapin
136
16
9
Clonalité
Polyclonal
Conjugué
Cet anticorp TACR1 est non-conjugé
8
8
7
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
108
59
57
32
25
19
17
13
5
5
2
2
1
Fournisseur
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N° du produit (Fournisseur)
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Images

N° du produit ABIN152451
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
1 ABIN307532 IHC IHC (fro) Rabbit IgG C-Term Connectez-vous pour afficher Polyclonal 0
1 ABIN307533 ICC IHC IHC (fro) Rabbit IgG C-Term Connectez-vous pour afficher Polyclonal 0
1 ABIN326021 IHC IHC (fro) Rabbit IgG AA 385-407 Connectez-vous pour afficher Polyclonal 0
1 ABIN307534 IHC IHC (fro) Rabbit IgG Connectez-vous pour afficher Polyclonal 0
1 ABIN1737299 IHC (fro) IF WB Rabbit C-Term Connectez-vous pour afficher Polyclonal 0

General

Antigène Tachykinin Receptor 1 (TACR1) Anticorps
Épitope C-Term
(23), (18), (15), (14), (11), (8), (7), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Cobaye, Humain, Souris, Rat (Rattus)
(150), (71), (64), (10), (10), (6), (5), (5), (4), (4), (2), (2), (1), (1), (1), (1)
Hôte Lapin
(136), (16), (9)
Clonalité
Polyclonal
Conjugué Cet anticorp TACR1 est non-conjugé
(8), (8), (7), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(108), (59), (57), (32), (25), (19), (17), (13), (5), (5), (2), (2), (1)
Pubmed 31 références disponible
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Détail du produit anti-TACR1 anticorps

Détail du antigène TACR1 Information d'application Stockage References for anti-TACR1 antibody (ABIN152451) Images
Specificité This antibody is specific to the NK-1 receptor. Staining of cells transfected with NK-1 was abolished by preabsorption of the antisera with NK-1. Specificity was also verified by the localization of immunohistochemical staining with NB300-101 in neurons in
Purification Unpurified
Immunogène A synthetic peptide corresponding to the C-terminus of rat Neurokinin 1 Receptor, conjugated to bovine thyroglobulin. [UniProt# P14600]
Plasmids, Primers & others

Détail du antigène TACR1

Détail du produit anti-TACR1 anticorps Information d'application Stockage References for anti-TACR1 antibody (ABIN152451) Images Haut de la page
Antigène
Autre désignation NK1R (TACR1 Antibody Extrait)
Sujet Gene Symbol: TACR1
ID gène 6869
UniProt P14600
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration

Information d'application

Détail du produit anti-TACR1 anticorps Détail du antigène TACR1 Stockage References for anti-TACR1 antibody (ABIN152451) Images Haut de la page
Indications d'application Western Blot 1:100-1:2000, Immunohistochemistry 1:1000, Immunocytochemistry/Immunofluorescence 1:50, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:1000This Neurokinin 1 Receptor antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western Blot. For ICC/IF staining, please see customer the review. Use in Immunohistochemistry on paraffin embedded sections was reported in scientific literature (PMID: 23792204).
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Protocol specific for Neurokinin 1 Receptor Antibody Immunohistochemistry1) Perfuse rat through the ascending aorta with 500 mL. of 0.1 M phosphate buffered saline (PBS) (pH 7.4 at 4 degrees C), followed by 750 mL. of PBS containing 4 % formaldehyde and 12.5 % picric acid (pH 6.9, 4 degrees C). Tissue of interest is then dissected and post-fixed in PBS containing 4 % formaldehyde and 12.5 % picric acid (pH 6.9 at 4 degrees C) for at least 4 hours, followed by fixation in PBS containing 30 % sucrose (pH 7.4, 4 degrees C) for at least 24 hours. Until the tissue sinks.
2) Cut fixed sections at 60 um using a sliding microtome. 3) If using culture cells, remove media and perform 2-3 washed in PBS (pH 7.
) -use same protocol without agitation. 4) Place tissue section in microcentrifuge tubes containing 1 mL. Of PBS, pH 7.4 as they are being cut. Wash sections (in PBS) on an upright rotator for 10-15 minutes.
) Remove PBS and add 1 mL. of blocking solution (PBS + 1 % normal donkey serum (NDS) + 0.3 % triton X-100).
) Incubate tissue sections in blocking solution for 30 minutes at room temperature (RT) on rotator. 7) Remove blocking solution and add anti-neurokinin-1 receptor or anti-Neurokinin-3 receptor antibody +PBS + 1 % NDS + 0.3 % triton X-100 + NB 300-101/102 at 1:100~1:10008) Incubate overnight on rotator at RT.
) Remove primary antisera and perform 3 x 10 minute PBS washes (1ml volume) on rotator.
) Add 1 mL. of secondary antibody and incubate for 2 hours at RT on rotator. -PBS + 1 % NDS + 0.3 % triton X 100 + anti-rabbit Cy3 (Jackson Immunoresearch Labs) at 1:60011) Perform 3 X 10 minute PBS washes (1 mL volume) on rotator12) Mount tissue sections on gelatin coated slides and allow tissue to dry13) Run slides through alcohol gradients (70 %, 90 %, 100 %, xylene) leaving slide in each alcohol and xylene for 2 minutes14) Coverslip with DPX mountant (FLUKA) ** Note: 24 well plates may be substituted for microcentrifuge tubes. Use flat top bench rotator instead of upright rotator.
Restrictions For Research Use only

Stockage

Détail du produit anti-TACR1 anticorps Détail du antigène TACR1 Information d'application References for anti-TACR1 antibody (ABIN152451) Images Haut de la page
Format Liquid
Buffer Whole antisera
Buffer contains: No Preservative
Agent conservateur Without preservative
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock -80 °C,-20 °C
Stockage commentaire Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-TACR1 antibody (ABIN152451)

Détail du produit anti-TACR1 anticorps Détail du antigène TACR1 Information d'application Stockage Images Haut de la page
Produit citée dans:

Li, Guo, Sun, Li, Ma, Zhang, Guan, Li, Wang: "Botulinum toxin type A and gabapentin attenuate postoperative pain and NK1 receptor internalization in rats." dans: Neurochemistry international, Vol. 116, pp. 52-62, 2018

Morinaga, Nakamuta, Yamamoto: "Hypoxia-induced increases in serotonin-immunoreactive nerve fibers in the medulla oblongata of the rat." dans: Acta histochemica, Vol. 118, Issue 8, pp. 806-817, 2016 (Échantillon (espèces): Human).

Wu, Zhang, Qian, Guo, Yang, Huang, Luo, Xiang, Deng, Dai, Peng, Pan: "Antinociceptive effects of endomorphin-2: suppression of substance P release in the inflammatory pain model rat." dans: Neurochemistry international, Vol. 82, pp. 1-9, 2015 (Échantillon (espèces): Rat (Rattus)). Détails: Immunohistochemistry (Frozen Sections)

Li, Fotinos, Wu, Chen, Zhu, Baranov, Tu, Zhou, Sinha, Kristal, Wang: "N-acetyl-L-tryptophan delays disease onset and extends survival in an amyotrophic lateral sclerosis transgenic mouse model." dans: Neurobiology of disease, Vol. 80, pp. 93-103, 2015 (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Grip, Lonne-Rahm, Holst, Johansson, Nordlind, Theodorsson, El-Nour: "Substance P alterations in skin and brain of chronically stressed atopic-like mice." dans: Journal of the European Academy of Dermatology and Venereology : JEADV, Vol. 27, Issue 2, pp. 199-205, 2013 (Échantillon (espèces): Mouse (Murine)). Détails: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Ramalho, Almeida, Fernandes, Costa, Pirraco, Guardão, Delgado, Moreira, Soares: "Neurokinin-1 receptor, a new modulator of lymphangiogenesis in obese-asthma phenotype." dans: Life sciences, Vol. 93, Issue 4, pp. 169-77, 2013 (Échantillon (espèces): Mouse (Murine)). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Luo, Li, Zhang, Zheng, Wang, Nie, Zhang, Su: "Effects of four antitussives on airway neurogenic inflammation in a guinea pig model of chronic cough induced by cigarette smoke exposure." dans: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 62, Issue 12, pp. 1053-61, 2013 (Échantillon (espèces): Guinea Pig). Détails: Western Blotting

Yang, Yan, Zhang, Wang: "Substance P participates in immune-mediated hepatic injury induced by concanavalin A in mice and stimulates cytokine synthesis in Kupffer cells." dans: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 459-464, 2013 (Échantillon (espèces): Mouse (Murine)). Détails: Immunocytochemistry,Immunofluorescence

Lin, Moore, Jones, McGlashon, Talman: "Astrocytes in the rat nucleus tractus solitarii are critical for cardiovascular reflex control." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 47, pp. 18608-17, 2013 (Échantillon (espèces): Rat (Rattus)). Détails: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Talman, Dragon, Jones, Moore, Lin: "Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat." dans: Cellular and molecular neurobiology, Vol. 32, Issue 7, pp. 1119-26, 2012 (Échantillon (espèces): Rat (Rattus)). Détails: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Caioli, Curcio, Pieri, Antonini, Marolda, Severini, Zona: "Substance P receptor activation induces downregulation of the AMPA receptor functionality in cortical neurons from a genetic model of Amyotrophic Lateral Sclerosis." dans: Neurobiology of disease, Vol. 44, Issue 1, pp. 92-101, 2011 (Échantillon (espèces): Mouse (Murine)). Détails: Immunocytochemistry,Immunofluorescence

Commons: "Locally collateralizing glutamate neurons in the dorsal raphe nucleus responsive to substance P contain vesicular glutamate transporter 3 (VGLUT3)." dans: Journal of chemical neuroanatomy, Vol. 38, Issue 4, pp. 273-81, 2009 (Échantillon (espèces): Rat (Rattus)). Détails: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Amadoro, Pieri, Ciotti, Carunchio, Canu, Calissano, Zona, Severini: "Substance P provides neuroprotection in cerebellar granule cells through Akt and MAPK/Erk activation: evidence for the involvement of the delayed rectifier potassium current." dans: Neuropharmacology, Vol. 52, Issue 6, pp. 1366-77, 2007 (Échantillon (espèces): Rat (Rattus)).

Broccardo, Linari, Agostini, Amadoro, Carpino, Ciotti, Petrella, Petrozza, Severini, Improta: "Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 291, Issue 3, pp. G518-24, 2006 (Échantillon (espèces): Rat (Rattus)). Détails: Immunocytochemistry,Western Blotting,Immunofluorescence

Broccardo, Ciotti, Linari, Agostini, Petrella, Amadoro, Severini, Improta: "Immunocytochemical distribution of NK-1 and NK-3 tachykinin receptors in isolated pancreatic acini of guinea pigs and rats." dans: Peptides, Vol. 26, Issue 11, pp. 2351-4, 2005

Commons, Connolley, Valentino: "A neurochemically distinct dorsal raphe-limbic circuit with a potential role in affective disorders." dans: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 28, Issue 2, pp. 206-15, 2003 (Échantillon (espèces): Rat (Rattus)). Détails: Immunocytochemistry,Immunofluorescence

Mounir, Parent: "The expression of neurokinin-1 receptor at striatal and pallidal levels in normal human brain." dans: Neuroscience research, Vol. 44, Issue 1, pp. 71-81, 2002 (Échantillon (espèces): Human). Détails: Western Blotting,Immunohistochemistry

Ma, Huang, Chien, Wu, Chen: "Temporal decrease in renal sensory responses in rats after chronic ligation of the bile duct." dans: American journal of physiology. Renal physiology, Vol. 283, Issue 1, pp. F164-72, 2002 (Échantillon (espèces): Rat (Rattus)). Détails: Western Blotting

Kc, Haxhiu, Tolentino-Silva, Wu, Trouth, Mack: "Paraventricular vasopressin-containing neurons project to brain stem and spinal cord respiratory-related sites." dans: Respiratory physiology & neurobiology, Vol. 133, Issue 1-2, pp. 75-88, 2002

Yip, Chahl: "Localization of NK1 and NK3 receptors in guinea-pig brain." dans: Regulatory peptides, Vol. 98, Issue 1-2, pp. 55-62, 2001 (Échantillon (espèces): Guinea Pig). Détails: Immunohistochemistry

Images

Détail du produit anti-TACR1 anticorps Détail du antigène TACR1 Information d'application Stockage References for anti-TACR1 antibody (ABIN152451) Haut de la page
Images (Fournisseur)
Immunofluorescence (IF) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Confocal image of SPR reactivity using polyclonal anti-NK-1 Receptor (ABIN152451) in ...
Immunofluorescence (IF) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [ABIN268373] -...
Immunofluorescence (IF) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [ABIN268373] -...
Immunohistochemistry (IHC) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Immunohistochemistry: Neurokinin 1 Receptor Antibody [ABIN268373] - Immunohistochemic...