anti-Souris Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa anticorps pour Immunofluorescence

Recommended Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa Antibody

Antigène
Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) Anticorps
  • Pol2rb
  • Rpb140
  • Rpb2
  • POL2RB
  • RPB2
  • hRPB140
  • hsRPB2
  • RNA polymerase II subunit B
  • polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
  • DNA-directed RNA polymerase II subunit RPB2
  • polymerase (RNA) II (DNA directed) polypeptide B
  • polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog
  • POLR2B
  • polr2b
  • LOC100640211
  • Polr2b
  • polr2b.L
  • rpb-2
Épitope
N-Term
9
8
7
7
3
3
1
1
1
1
Reactivité
Humain, Souris
46
19
18
8
7
4
4
4
3
3
3
2
2
Hôte
Lapin
46
Clonalité
Polyclonal
Conjugué
Inconjugué
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
46
21
13
4
3
2
2
1
Options

Images

N° du produit ABIN2855420
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
-13.157621 ABIN4963453 ICC IF IHC IHC (p) WB Rabbit N-Term Polyclonal 0

General

Antigène Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) Anticorps
Épitope N-Term
(9), (8), (7), (7), (3), (3), (1), (1), (1), (1)
Reactivité Humain, Souris
(46), (19), (18), (8), (7), (4), (4), (4), (3), (3), (3), (2), (2)
Hôte Lapin
(46)
Clonalité
Polyclonal
Conjugué Inconjugué
(2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(46), (21), (13), (4), (3), (2), (2), (1)
Fournisseur

Détail du produit

Détail du antigène Information d'application Stockage Images
 Réactivité croisée Rat (Rattus), Macaque rhésus, Boeuf (Vache)
Réactivité croisée (Details) Rat (100 %), Rhesus Monkey (100 %), Bovine (100 %)
Attributs du produit Rabbit Polyclonal antibody to RNA polymerase IIB (DNA-directed RNA polymerase II subunit RPB2)
RPB2 antibody [N1], N-term
Purification Purified by antigen-affinity chromatography.
Immunogène Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
Isotype IgG

Détail du antigène

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Antigène
Autre désignation RPB2 (POLR2B Antibody Extrait)
Sujet This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Cellular Localization: Nucleus
Poids moléculaire 134 kDa
ID gène 5431
Pathways Regulatory RNA Pathways, Réparation de l'ADN

Information d'application

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Indications d'application Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Commentaires

Positive Control: H1299 , BCL-1

Restrictions For Research Use only

Stockage

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Format Liquid
Concentration 0.65 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Agent conservateur Thimerosal (Merthiolate)
Précaution d'utilisation This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

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Images (Fournisseur)
Immunofluorescence (IF) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibody (ABIN2855420) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPB2, ...
Western Blotting (WB) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibody (ABIN2855420) WB Image Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE antibody diluted at...
Immunohistochemistry (IHC) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibody (ABIN2855420) IHC-P Image Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using...
Western Blotting (WB) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibody (ABIN2855420) WB Image Sample (30 ug of whole cell lysate) A: BCL-1 5% SDS PAGE antibody diluted at...