anti-Humain E-cadherin anticorps pour BioImaging

Recommended E-cadherin Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) Anticorps
  • Arc-1
  • CD324
  • CDHE
  • ECAD
  • LCAM
  • UVO
  • E-cadherin
  • uvo
  • lcam
  • E-Cad
  • XBcad
  • l-cam
  • xcdh1
  • cdhc-A
  • xb-cad
  • XTCAD-1
  • c-cadherin
  • cadherin-1
  • uvomorulin
  • XB-cadherin
  • arc-1
  • cd324
  • cdhe
  • ecad
  • AA960649
  • E-cad
  • Ecad
  • L-CAM
  • Um
  • cadherin 1
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin 1, type 1 S homeolog
  • CDH1
  • cdh1
  • Cdh1
  • cdh1.S
Reactivité
Humain
797
239
144
40
26
16
9
8
8
7
6
4
3
1
1
1
1
1
1
1
1
Hôte
Souris
542
265
37
22
2
Clonalité
Monoclonal
Conjugué
Cet anticorp E-cadherin est non-conjugé
43
39
34
31
24
21
21
17
17
15
13
13
13
13
12
12
11
10
8
7
7
6
4
3
3
3
3
3
3
2
2
Application
BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
645
370
275
213
193
176
157
87
81
38
19
14
13
12
10
7
6
6
4
2
2
2
2
2
1
1
Options
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N° du produit ABIN2689191
Produit non disponible pour cette région.

General

Antigène Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) Anticorps
Reactivité Humain
(797), (239), (144), (40), (26), (16), (9), (8), (8), (7), (6), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Hôte Souris
(542), (265), (37), (22), (2)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp E-cadherin est non-conjugé
(43), (39), (34), (31), (24), (21), (21), (17), (17), (15), (13), (13), (13), (13), (12), (12), (11), (10), (8), (7), (7), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2)
Application BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(645), (370), (275), (213), (193), (176), (157), (87), (81), (38), (19), (14), (13), (12), (10), (7), (6), (6), (4), (2), (2), (2), (2), (2), (1), (1)
Pubmed 3 références disponible
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Détail du produit anti-E-cadherin anticorps

Détail du antigène E-cadherin Information d'application Stockage References for anti-E-cadherin antibody (ABIN2689191) Images
Marque BD Pharmingen™
Attributs du produit The 67A4 monoclonal antibody recognizes the extracellular domain of human E-Cadherin (CD324). E-Cadherin is a 120- kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-to-cell adhesion. These E-Cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces their invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. Pluripotent stem cells express E-Cadherin. Upon differentiation, an epithelial to mesenchymal transition results in the loss of E-cadherin expression and a gain in the expression of N-cadherin. Western blot analysis of CD324 (E-cadherin) expression in human breast adenocarcinoma and human embryonic stem (ES) cells. Cell Lysates from a human breast adenocarcinoma cell line MCF-7 (ATCC HTB-22, left blot) and H9 human ES Cells (WiCell, Madison, WI, right blot) were probed with Purified Mouse Anti-Human CD324 (E-Cadherin) monoclonal antibody at titrations of 0.125 (lanes 1), 0.063 (lanes 2), 0.032 (lanes 3) μg/mL. Proteins were detected using HRP Goat Anti-Mouse Ig (Cat. No. 554002). CD324 (E-cadherin) is identified as a band of ~120 kDa in MCF7 and Human ES cells. Immunoflourescent staining of CD324 (E-cadherin) on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 45 grown in mTESR™1 media (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). Cells were stained with Purified Mouse Anti-Human CD324 (E-Cadherin) monoclonal antibody (pseudo-colored green) at 2.5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and cell nuclei were stained with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software.

BD Pharmingen™ Purified Mouse Anti-Human CD324 (E-Cadherin) - Purified - Clone 67A4 - Isotype Mouse IgG1, κ - Reactivity Hu - 50 μg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogène Human Breast Tumor Cell Line
Clone 67A4
Isotype IgG1, kappa

Détail du antigène E-cadherin

Détail du produit anti-E-cadherin anticorps Information d'application Stockage References for anti-E-cadherin antibody (ABIN2689191) Images Haut de la page
Antigène
Autre désignation CD324 E-Cadherin (CDH1 Antibody Extrait)
Sujet Synonyms: E-cadherin
Poids moléculaire ~120 kDa
Pathways Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation

Information d'application

Détail du produit anti-E-cadherin anticorps Détail du antigène E-cadherin Stockage References for anti-E-cadherin antibody (ABIN2689191) Images Haut de la page
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Stockage

Détail du produit anti-E-cadherin anticorps Détail du antigène E-cadherin Information d'application References for anti-E-cadherin antibody (ABIN2689191) Images Haut de la page
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Stockage commentaire Store undiluted at 4°C.

References for anti-E-cadherin antibody (ABIN2689191)

Détail du produit anti-E-cadherin anticorps Détail du antigène E-cadherin Information d'application Stockage Images Haut de la page
Produit citée dans:

DAmour, Agulnick, Eliazer, Kelly, Kroon, Baetge: "Efficient differentiation of human embryonic stem cells to definitive endoderm." dans: Nature biotechnology, Vol. 23, Issue 12, pp. 1534-41, 2005

Cepek, Shaw, Parker, Russell, Morrow, Rimm, Brenner: "Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta 7 integrin." dans: Nature, Vol. 372, Issue 6502, pp. 190-3, 1994

Takeichi: "The cadherins: cell-cell adhesion molecules controlling animal morphogenesis." dans: Development (Cambridge, England), Vol. 102, Issue 4, pp. 639-55, 1988