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General |
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Antigène | Smoothened Homolog (Drosophila) (SMO) Anticorps |
Épitope | N-Term Alternatives |
Reactivité | Roussette (Chauve-souris), Boeuf (Vache), Chien, Hamster, Cheval, Humain, Singe, Souris, Porc, Lapin, Rat (Rattus) Alternatives |
Hôte | Lapin Alternatives |
Clonalité | |
Conjugué | Cet anticorp SMO est non-conjugé Alternatives |
Application |
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternatives
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1 référence disponible |
Fournisseur | Connectez-vous pour afficher |
Détail du produit anti-SMO anticorpsDétail du antigène SMO Information d'application Stockage References for anti-SMO antibody (ABIN1049353) Images |
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Marque | IHC-plus™ |
Specificité | Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
Purification | Immunoaffinity purified |
Immunogène |
Synthetic 15 amino acid peptide from N-terminal extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%), Opossum (93%), Turkey, Chicken, Xenopus (87%), Zebrafish, Seq squirt (80%). Type of Immunogen: Synthetic peptide |
Plasmids, Primers & others | |
Détail du antigène SMODétail du produit anti-SMO anticorps Information d'application Stockage References for anti-SMO antibody (ABIN1049353) Images Haut de la page |
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Antigène | |
Autre désignation | SMO / Smoothened (SMO Antibody Extrait) |
Sujet |
Name/Gene ID: SMO Subfamily: Frizzled Family: GPCR Synonyms: SMO, Frizzled family member 11, FZD11, Gx, Protein Gx, SMOH, Smoothened, Smoothened homolog |
ID gène | 6608 |
Pathways | Signalisation Hedgehog |
Information d'applicationDétail du produit anti-SMO anticorps Détail du antigène SMO Stockage References for anti-SMO antibody (ABIN1049353) Images Haut de la page |
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Indications d'application |
Approved: ICC, IF, IHC, IHC-P (10 μg/mL), WB Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. |
Commentaires |
Target Species of Antibody: Human |
Procédure de l'essai |
The IHC-pro Immunohistochemistry Protocol Tissue Preparation Formalin fixation and embedding in paraffin wax Tissue Sectioning Make 4-µm sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C Deparaffinization Wash slides in 3 changes of xylene – 5 minutes each at room temperature. Rehydration Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature. Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature. Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature. Rinse slides in gentle running distilled water – 5 minutes at room temperature. Antigen retrieval Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes Remove from heat and let stand at room temperature in buffer - 20 minutes Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature. Immunostaining Do not allow tissues to dry at any time during the staining procedure. Apply a universal protein block – 20 minutes at room temperature. Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature. Rinse slides 1X TBST – 1 minute at room temperature. Apply alkaline phosphatase streptavidin – 30 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature. Wash slides in distilled water – 1 minute at room temperature. Dehydrate This method should only be used if the chromogen substrate is alcohol insoluble. Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature. Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of xylene – 1 minute each at room temperature. Apply coverslip |
Restrictions | For Research Use only |
StockageDétail du produit anti-SMO anticorps Détail du antigène SMO Information d'application References for anti-SMO antibody (ABIN1049353) Images Haut de la page |
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Format | Liquid |
Concentration | Lot specific |
Buffer | PBS, less than 0.1 % sodium azide. |
Agent conservateur | Sodium azide |
Précaution d'utilisation | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Stock | 4 °C,-20 °C |
Stockage commentaire | Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles. |
Date de péremption | 12 months |
References for anti-SMO antibody (ABIN1049353)Détail du produit anti-SMO anticorps Détail du antigène SMO Information d'application Stockage Images Haut de la page |
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Background publications |
Corbit, Aanstad, Singla, Norman, Stainier, Reiter: "Vertebrate Smoothened functions at the primary cilium." dans: Nature, Vol. 437, Issue 7061, pp. 1018-21, 2005 |
ImagesDétail du produit anti-SMO anticorps Détail du antigène SMO Information d'application Stockage References for anti-SMO antibody (ABIN1049353) Haut de la page |
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Images (Fournisseur) |