anti-Humain ARAF anticorps pour Enzyme Immunoassay

Recommended ARAF Antibody (fourni par: Connectez-vous pour afficher )

Antigène
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Anticorps
  • zgc:92074
  • wu:fk45h07
  • ARAF
  • a-raf
  • araf
  • araf1
  • pks2
  • rafa1
  • A-RAF
  • ARAF1
  • PKS2
  • RAFA1
  • 1200013E08Rik
  • A-Raf
  • AW495444
  • Araf1
  • A-Raf proto-oncogene, serine/threonine kinase
  • A-Raf proto-oncogene, serine/threonine kinase L homeolog
  • A-Raf proto-oncogene, serine/threonine kinase S homeolog
  • Araf proto-oncogene, serine/threonine kinase
  • araf
  • ARAF
  • araf.L
  • araf.S
  • Araf
Épitope
Middle Region
25
24
16
15
11
11
10
10
9
7
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain
172
105
92
6
6
6
5
2
2
2
1
Hôte
Lapin
149
25
Clonalité
Polyclonal
Conjugué
Cet anticorp ARAF est non-conjugé
6
6
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
123
75
43
39
31
16
9
3
2
2
1
1
1
Fournisseur
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N° du produit (Fournisseur)
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N° du produit ABIN360127
$ 390.50
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General

Antigène V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) Anticorps
Épitope Middle Region
(25), (24), (16), (15), (11), (11), (10), (10), (9), (7), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain
(172), (105), (92), (6), (6), (6), (5), (2), (2), (2), (1)
Hôte Lapin
(149), (25)
Clonalité
Polyclonal
Conjugué Cet anticorp ARAF est non-conjugé
(6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(123), (75), (43), (39), (31), (16), (9), (3), (2), (2), (1), (1), (1)
Pubmed 4 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-ARAF anticorps

Détail du antigène ARAF Information d'application Stockage References for anti-ARAF antibody (ABIN360127) Images
Specificité This antibody reacts to A-RAF.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogène This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ARAF1.
Isotype Ig Fraction
Plasmids, Primers & others

Détail du antigène ARAF

Détail du produit anti-ARAF anticorps Information d'application Stockage References for anti-ARAF antibody (ABIN360127) Images Haut de la page
Antigène
Autre désignation ARAF (ARAF Antibody Extrait)
Sujet Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, ARAF1, PKS, PKS2, Proto-oncogene Pks
ID gène 369, 9606
UniProt P10398
Pathways Signalisation MAPK, Signalisation RTK, Hepatitis C

Information d'application

Détail du produit anti-ARAF anticorps Détail du antigène ARAF Stockage References for anti-ARAF antibody (ABIN360127) Images Haut de la page
Indications d'application ELISA: 1/1,000. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Stockage

Détail du produit anti-ARAF anticorps Détail du antigène ARAF Information d'application References for anti-ARAF antibody (ABIN360127) Images Haut de la page
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock 4 °C/-20 °C
Stockage commentaire Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-ARAF antibody (ABIN360127)

Détail du produit anti-ARAF anticorps Détail du antigène ARAF Information d'application Stockage Images Haut de la page
Background publications

Wu, Noh, Zhou, Dixon, Guan: "Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells." dans: The Journal of biological chemistry, Vol. 271, Issue 6, pp. 3265-71, 1996

Lee, Beck, Brennscheidt, DeGennaro, Rapp: "The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1)." dans: Genomics, Vol. 20, Issue 1, pp. 43-55, 1994

Popescu, Mark: "Localization of the pKs gene, a raf related sequence on human chromosomes X and 7." dans: Oncogene, Vol. 4, Issue 4, pp. 517-9, 1989

Beck, Huleihel, Gunnell, Bonner, Rapp: "The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus." dans: Nucleic acids research, Vol. 15, Issue 2, pp. 595-609, 1987

Images

Détail du produit anti-ARAF anticorps Détail du antigène ARAF Information d'application Stockage References for anti-ARAF antibody (ABIN360127) Haut de la page
Images (Fournisseur)
 image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Middle Region) antibody (ABIN360127) anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Middle Region) antibody