We are preparing the requested document. Please wait, this may take a while...!
|Antigène||Heat Shock 27kDa Protein 1 (HSPB1) Anticorps|
|Reactivité||Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Souris, Rat (Rattus) Alternatives|
|Conjugué||Cet anticorp HSPB1 est non-conjugé Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|Fournisseur||Connectez-vous pour afficher|
Détail du produit anti-HSPB1 anticorpsDétail du antigène HSPB1 Information d'application Stockage Images
|Specificité||Detects a 25 kDa or 27 kDa protein corresponding to the Molecular Mass of Hsp25/27 on SDS PAGE immunoblots. Cross-reacts with Alpha B Crystallin.|
|Attributs du produit||Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein|
Détail du antigène HSPB1Détail du produit anti-HSPB1 anticorps Information d'application Stockage Images Haut de la page
|Autre désignation||HSPB1 / HSP27 (HSPB1 Antibody Extrait)|
|Sujet||Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27|
|Pathways||Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling|
Information d'applicationDétail du produit anti-HSPB1 anticorps Détail du antigène HSPB1 Stockage Images Haut de la page
Western blot (6): 1: 5000 (ECL). Immunoflourescence (6): 1: 200. Immunoprecipitation (9). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
StockageDétail du produit anti-HSPB1 anticorps Détail du antigène HSPB1 Information d'application Images Haut de la page
|Buffer||PBS, 0.09 % Sodium Azide, 50 % Glycerol|
|Agent conservateur||Sodium azide|
|Précaution d'utilisation||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Stock||4 °C/-20 °C|
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
|Date de péremption||12 months|