anti-Rat (Rattus) HSPB1 anticorps pour Western Blotting

Recommended HSPB1 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Heat Shock 27kDa Protein 1 (HSPB1) Anticorps
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Reactivité
Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Souris, Rat (Rattus), Mouton
825
276
271
95
85
71
67
65
36
29
29
25
18
11
11
9
6
3
2
2
2
2
1
1
1
Hôte
Souris
503
340
2
Clonalité
Monoclonal
Conjugué
Cet anticorp HSPB1 est non-conjugé
34
33
23
22
19
13
13
13
11
11
10
10
10
10
10
9
9
9
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
714
326
270
266
227
211
168
156
59
40
17
14
11
8
6
4
2
2
1
1
1
Options
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N° du produit ABIN361756
$ 334.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
3.3322737 ABIN4319980 ICC IF IHC IHC (p) WB Rabbit AA 80-150, Internal Region Connectez-vous pour afficher Polyclonal 2
3.3322737 ABIN1531868 ELISA WB Rabbit IgG AA 48-97, pSer82 Connectez-vous pour afficher Polyclonal
3.3322737 ABIN1532899 IHC ELISA WB Rabbit IgG AA 48-97 Connectez-vous pour afficher Polyclonal
3.3322737 ABIN969199 ICC FACS IHC ELISA WB Mouse IgG1 Connectez-vous pour afficher 5D7 1
3.3322737 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Connectez-vous pour afficher Polyclonal
3.3322737 ABIN3182035 ELISA IHC WB Rabbit IgG pSer82 Connectez-vous pour afficher Polyclonal
3.3322737 ABIN3185106 ELISA IHC WB Rabbit IgG Ser505 Connectez-vous pour afficher Polyclonal
3.3322737 ABIN126812 IHC (fro) WB Mouse IgG2a pSer82 Connectez-vous pour afficher 5B9
3.3322737 ABIN3181506 IHC WB Rabbit IgG Connectez-vous pour afficher Polyclonal
3.3322737 ABIN1873103 IF WB Rabbit IgG Connectez-vous pour afficher Polyclonal
3.3322737 ABIN4320008 CyTOF FACS ICC IF WB Mouse IgG1 Connectez-vous pour afficher 6H11
3.3322737 ABIN3044184 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 88-105, Middle Region Connectez-vous pour afficher Polyclonal
3.3322737 ABIN4903971 IHC WB Mouse IgG Connectez-vous pour afficher
3.3322737 ABIN222928 WB Rabbit IgG Connectez-vous pour afficher Polyclonal
3.3322737 ABIN267333 ICC IF IHC WB Rabbit IgG Connectez-vous pour afficher Polyclonal
3.3322737 ABIN4319976 ELISA FACS ICC IF IHC WB Mouse IgG1 Connectez-vous pour afficher 5D7
3.3322737 ABIN1107614 EIA IF IHC (p) WB Mouse IgG1 Connectez-vous pour afficher 5D7 2
3.3322737 ABIN5611604 EIA IHC (p) WB Rabbit IgG Connectez-vous pour afficher Polyclonal
3.3322737 ABIN498746 IHC (p) WB Rabbit pSer82 Connectez-vous pour afficher Polyclonal
3.3322737 ABIN4320010 ICC IF WB Chicken Connectez-vous pour afficher Polyclonal

General

Antigène Heat Shock 27kDa Protein 1 (HSPB1) Anticorps
Reactivité Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Souris, Rat (Rattus), Mouton
(825), (276), (271), (95), (85), (71), (67), (65), (36), (29), (29), (25), (18), (11), (11), (9), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Hôte Souris
(503), (340), (2)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp HSPB1 est non-conjugé
(34), (33), (23), (22), (19), (13), (13), (13), (11), (11), (10), (10), (10), (10), (10), (9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(714), (326), (270), (266), (227), (211), (168), (156), (59), (40), (17), (14), (11), (8), (6), (4), (2), (2), (1), (1), (1)
Pubmed 9 références disponible
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Détail du produit anti-HSPB1 anticorps

Détail du antigène HSPB1 Information d'application Stockage References for anti-HSPB1 antibody (ABIN361756) Images
Specificité Detects ~25 kDa or ~27 kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Purification Protein G Purified
Immunogène HSP27 peptide
Clone 8A7
Isotype IgG1 kappa

Détail du antigène HSPB1

Détail du produit anti-HSPB1 anticorps Information d'application Stockage References for anti-HSPB1 antibody (ABIN361756) Images Haut de la page
Antigène
Autre désignation HSP27 (HSPB1 Antibody Extrait)
Sujet HSP25 is the mouse homologue of the human HSP27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, HSP27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (8). For more information visit our HSP27 Scientific Resource Guide at http://www.HSP27.com.
ID gène 3315
NCBI Accession NP_001532
UniProt P04792
Domaine de recherche Heat Shock Proteins
Pathways Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Information d'application

Détail du produit anti-HSPB1 anticorps Détail du antigène HSPB1 Stockage References for anti-HSPB1 antibody (ABIN361756) Images Haut de la page
Indications d'application
  • WB (1:5000)
  • ICC/IF (1:200)
  • IHC (1:100)
  • optimal dilutions for assays should be determined by the user.
Commentaires

A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions For Research Use only

Stockage

Détail du produit anti-HSPB1 anticorps Détail du antigène HSPB1 Information d'application References for anti-HSPB1 antibody (ABIN361756) Images Haut de la page
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C

References for anti-HSPB1 antibody (ABIN361756)

Détail du produit anti-HSPB1 anticorps Détail du antigène HSPB1 Information d'application Stockage Images Haut de la page
Background publications

Arrigo: "In search of the molecular mechanism by which small stress proteins counteract apoptosis during cellular differentiation." dans: Journal of cellular biochemistry, Vol. 94, Issue 2, pp. 241-6, 2005

Van Montfort, Slingsby, Vierling: "Structure and function of the small heat shock protein/alpha-crystallin family of molecular chaperones." dans: Advances in protein chemistry, Vol. 59, pp. 105-56, 2002

Jia, Ransom, Shibanuma, Liu, Welsh, Smoyer: "Identification and characterization of hic-5/ARA55 as an hsp27 binding protein." dans: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 39911-8, 2001

Sarto, Binz, Mocarelli: "Heat shock proteins in human cancer." dans: Electrophoresis, Vol. 21, Issue 6, pp. 1218-26, 2000

Kim, Kim, Kim: "Crystal structure of a small heat-shock protein." dans: Nature, Vol. 394, Issue 6693, pp. 595-9, 1998

Ehrnsperger, Gräber, Gaestel, Buchner: "Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation." dans: The EMBO journal, Vol. 16, Issue 2, pp. 221-9, 1997

Welsh, Wu, Parvinen, Gilmont: "Variation in expression of hsp27 messenger ribonucleic acid during the cycle of the seminiferous epithelium and co-localization of hsp27 and microfilaments in Sertoli cells of the rat." dans: Biology of reproduction, Vol. 55, Issue 1, pp. 141-51, 1997

Ciocca, Oesterreich, Chamness, McGuire, Fuqua: "Biological and clinical implications of heat shock protein 27,000 (Hsp27): a review." dans: Journal of the National Cancer Institute, Vol. 85, Issue 19, pp. 1558-70, 1993

Welch: "Phorbol ester, calcium ionophore, or serum added to quiescent rat embryo fibroblast cells all result in the elevated phosphorylation of two 28,000-dalton mammalian stress proteins." dans: The Journal of biological chemistry, Vol. 260, Issue 5, pp. 3058-62, 1985

Images

Détail du produit anti-HSPB1 anticorps Détail du antigène HSPB1 Information d'application Stockage References for anti-HSPB1 antibody (ABIN361756) Haut de la page
Supplier Images
 image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN361756) Hsp25 Hsp27 (8A7), somites of a rat embryo Courtesy of Mike Welsh, UMich .
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN361756) SMC 114 Rat LungTissue 10ug Hsp25 WB 1 in 1000.