anti-Requin HSPB1 anticorps pour Immunocytochemistry

Recommended HSPB1 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Heat Shock 27kDa Protein 1 (HSPB1) Anticorps
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Reactivité
Chien, Humain, Souris, Requin
981
365
340
156
127
103
89
68
67
54
49
44
35
30
13
11
6
4
3
2
2
2
1
1
1
1
Hôte
Lapin
561
440
3
Clonalité
Polyclonal
Conjugué
Cet anticorp HSPB1 est non-conjugé
42
40
29
28
24
18
16
13
13
12
10
10
10
10
10
9
9
9
8
8
8
8
8
8
8
8
8
6
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
860
446
304
285
272
234
216
209
75
58
42
42
40
18
14
6
5
4
2
2
1
1
1
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Request

Recevoir ce produit gratuitement

Envoyez-nous votre proposition de validation. J'aimerais valider ce produit

Savoir plus

Images

N° du produit ABIN1027725
$ 183.70
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
1 ABIN2484360 FACS ICC IF WB PE Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484345 FACS ICC IF WB Atto 390 Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484347 FACS ICC IF WB Atto 565 Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484348 FACS ICC IF WB Atto 594 Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484349 FACS ICC IF WB Atto 633 Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484350 FACS ICC IF WB Atto 655 Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484352 FACS ICC IF WB Atto 700 Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484354 FACS ICC IF WB APC Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484355 FACS ICC IF WB Biotin Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484356 FACS ICC IF WB FITC Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484359 FACS ICC IF WB PerCP Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484358 FACS ICC IF WB PE-Atto 594 Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484361 FACS ICC IF WB Streptavidin Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484346 FACS ICC IF WB Atto 488 Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484351 FACS ICC IF WB Atto 680 Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484353 FACS ICC IF WB Alkaline Phosphatase (AP) Rabbit Connectez-vous pour afficher Polyclonal 6
1 ABIN2484357 FACS ICC IF WB HRP Rabbit Connectez-vous pour afficher Polyclonal 6

General

Antigène Heat Shock 27kDa Protein 1 (HSPB1) Anticorps
Reactivité Chien, Humain, Souris, Requin
(981), (365), (340), (156), (127), (103), (89), (68), (67), (54), (49), (44), (35), (30), (13), (11), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Hôte Lapin
(561), (440), (3)
Clonalité
Polyclonal
Conjugué Cet anticorp HSPB1 est non-conjugé
(42), (40), (29), (28), (24), (18), (16), (13), (13), (12), (10), (10), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(860), (446), (304), (285), (272), (234), (216), (209), (75), (58), (42), (42), (40), (18), (14), (6), (5), (4), (2), (2), (1), (1), (1)
Pubmed 7 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-HSPB1 anticorps

Détail du antigène HSPB1 Information d'application Stockage References for anti-HSPB1 antibody (ABIN1027725) Images
Specificité Detects ~27 kDa.
Purification Protein A Purified
Immunogène Human HSP27, His tagged
Plasmids, Primers & others

Détail du antigène HSPB1

Détail du produit anti-HSPB1 anticorps Information d'application Stockage References for anti-HSPB1 antibody (ABIN1027725) Images Haut de la page
Antigène
Autre désignation HSP27 (HSPB1 Antibody Extrait)
Sujet HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermo tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6). Looking for more information on HSP27? Visit our new HSP27 Scientific Resource Guide at http://www.HSP27.com.
ID gène 3315
NCBI Accession NP_001531
UniProt P04792
Pathways Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Information d'application

Détail du produit anti-HSPB1 anticorps Détail du antigène HSPB1 Stockage References for anti-HSPB1 antibody (ABIN1027725) Images Haut de la page
Indications d'application
  • WB (1:2000)
  • ICC/IF (1:250)
  • optimal dilutions for assays should be determined by the user.
Commentaires

A 1:2000 dilution of SPC-106 was sufficient for detection of HSP27 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions For Research Use only

Stockage

Détail du produit anti-HSPB1 anticorps Détail du antigène HSPB1 Information d'application References for anti-HSPB1 antibody (ABIN1027725) Images Haut de la page
Format Liquid
Concentration 1.38 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C

References for anti-HSPB1 antibody (ABIN1027725)

Détail du produit anti-HSPB1 anticorps Détail du antigène HSPB1 Information d'application Stockage Images Haut de la page
Produit citée dans:

Asai, Kawashima, Katagiri, Takeuchi, Tohnai, Ohtsuka: "Protective effect of a molecular chaperone inducer, paeoniflorin, on the HCl- and ethanol-triggered gastric mucosal injury." dans: Life sciences, Vol. 88, Issue 7-8, pp. 350-7, 2011

Background publications

Arrigo: "In search of the molecular mechanism by which small stress proteins counteract apoptosis during cellular differentiation." dans: Journal of cellular biochemistry, Vol. 94, Issue 2, pp. 241-6, 2005

Van Montfort, Slingsby, Vierling: "Structure and function of the small heat shock protein/alpha-crystallin family of molecular chaperones." dans: Advances in protein chemistry, Vol. 59, pp. 105-56, 2002

Sarto, Binz, Mocarelli: "Heat shock proteins in human cancer." dans: Electrophoresis, Vol. 21, Issue 6, pp. 1218-26, 2000

Kim, Kim, Kim: "Crystal structure of a small heat-shock protein." dans: Nature, Vol. 394, Issue 6693, pp. 595-9, 1998

Ehrnsperger, Gräber, Gaestel, Buchner: "Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation." dans: The EMBO journal, Vol. 16, Issue 2, pp. 221-9, 1997

Ciocca, Oesterreich, Chamness, McGuire, Fuqua: "Biological and clinical implications of heat shock protein 27,000 (Hsp27): a review." dans: Journal of the National Cancer Institute, Vol. 85, Issue 19, pp. 1558-70, 1993

Images

Détail du produit anti-HSPB1 anticorps Détail du antigène HSPB1 Information d'application Stockage References for anti-HSPB1 antibody (ABIN1027725) Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN1027725) Hsp27 Hela Western Blotting 1 in 2000.