anti-Humain MAPK3 anticorps pour Immunofluorescence

Recommended MAPK3 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Mitogen-Activated Protein Kinase 3 (MAPK3) Anticorps
  • ERK-1
  • ERK1
  • ERT2
  • HS44KDAP
  • HUMKER1A
  • P44ERK1
  • P44MAPK
  • PRKM3
  • p44-ERK1
  • p44-MAPK
  • Erk-1
  • Erk1
  • Ert2
  • Esrk1
  • Mnk1
  • Mtap2k
  • Prkm3
  • p44
  • p44erk1
  • p44mapk
  • fi06b09
  • wu:fi06b09
  • zERK1
  • Tb08.10J17.940
  • MAPK1
  • MNK1
  • ATMAPK3
  • ATMPK3
  • T6D9.4
  • mitogen-activated protein kinase 3
  • mitogen-activated protein kinase 3
  • mitogen activated protein kinase 3
  • mitogen-activated serine/threonine-protein kinase
  • MAPK3
  • Mapk3
  • mapk3
  • Tc00.1047053509475.10
  • Tb927.8.3550
  • CEK1
  • MPK3
Reactivité
Poulet, Boeuf (Vache), Mouche des fruits (Drosophila melanogaster), Humain, Souris, Rat (Rattus), Mouton, Xenopus laevis
512
288
261
50
41
37
37
23
20
19
18
10
10
9
9
8
7
4
3
2
2
2
1
1
1
1
Hôte
Lapin
399
117
19
6
1
Clonalité
Polyclonal
Conjugué
Cet anticorp MAPK3 est conjugé à/à la Atto 633
29
26
25
15
14
14
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
443
206
200
89
75
64
61
39
37
35
15
13
8
4
3
2
1
1
1
Options
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N° du produit (Fournisseur)
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N° du produit ABIN2486608
$ 458.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
31.280954 ABIN2486605 ICC FACS IF IHC WB Atto 488 Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486607 ICC FACS IF IHC WB Atto 594 Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486924 ICC FACS IF IHC WB PerCP Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486604 ICC FACS IF IHC WB Atto 390 Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486606 ICC FACS IF IHC WB Atto 565 Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486609 ICC FACS IF IHC WB Atto 655 Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486610 ICC FACS IF IHC WB Atto 680 Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486611 ICC FACS IF IHC WB Atto 700 Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486919 ICC FACS IF IHC WB APC Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486923 ICC FACS IF IHC WB PE-Atto 594 Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486921 ICC FACS IF IHC WB FITC Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486920 ICC FACS IF IHC WB Biotin Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486926 ICC FACS IF IHC WB Streptavidin Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486922 ICC FACS IF IHC WB HRP Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486925 ICC FACS IF IHC WB PE Rabbit Connectez-vous pour afficher Polyclonal 3
31.280954 ABIN2486918 ICC FACS IF IHC WB Alkaline Phosphatase (AP) Rabbit Connectez-vous pour afficher Polyclonal 3
31.235067 ABIN3019890 IF IHC WB Rabbit pTyr204 Connectez-vous pour afficher Polyclonal 1
31.235067 ABIN3019887 IF IHC WB Rabbit pThr202 Connectez-vous pour afficher Polyclonal 1
23.735067 ABIN934066 IF ELISA WB Mouse IgG2b kappa AA 1-137 Connectez-vous pour afficher AT1A2 0
23.454113 ABIN5583086 ELISA FACS IF IHC (p) WB Mouse IgG1 Connectez-vous pour afficher 1E5 0

General

Antigène Mitogen-Activated Protein Kinase 3 (MAPK3) Anticorps
Reactivité Poulet, Boeuf (Vache), Mouche des fruits (Drosophila melanogaster), Humain, Souris, Rat (Rattus), Mouton, Xenopus laevis
(512), (288), (261), (50), (41), (37), (37), (23), (20), (19), (18), (10), (10), (9), (9), (8), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Hôte Lapin
(399), (117), (19), (6), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp MAPK3 est conjugé à/à la Atto 633
(29), (26), (25), (15), (14), (14), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(443), (206), (200), (89), (75), (64), (61), (39), (37), (35), (15), (13), (8), (4), (3), (2), (1), (1), (1)
Pubmed 3 références disponible
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Détail du produit anti-MAPK3 anticorps

Détail du antigène MAPK3 Information d'application Stockage References for anti-MAPK3 antibody (ABIN2486608) Images
Specificité Detects ~44kda (ERK1) and ~42 kDa (ERK2).
Purification Peptide Affinity Purified
Immunogène A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Plasmids, Primers & others

Détail du antigène MAPK3

Détail du produit anti-MAPK3 anticorps Information d'application Stockage References for anti-MAPK3 antibody (ABIN2486608) Images Haut de la page
Antigène
Autre désignation ERK1 (MAPK3 Antibody Extrait)
Sujet The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
ID gène 50689
NCBI Accession NP_059043
UniProt P21708
Pathways Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Information d'application

Détail du produit anti-MAPK3 anticorps Détail du antigène MAPK3 Stockage References for anti-MAPK3 antibody (ABIN2486608) Images Haut de la page
Indications d'application
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:100)
  • FCM (1:100)
  • optimal dilutions for assays should be determined by the user.
Commentaires

A 1:1000 dilution of SPC-120 was sufficient for detection of ERK1/2 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions For Research Use only

Stockage

Détail du produit anti-MAPK3 anticorps Détail du antigène MAPK3 Information d'application References for anti-MAPK3 antibody (ABIN2486608) Images Haut de la page
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C

References for anti-MAPK3 antibody (ABIN2486608)

Détail du produit anti-MAPK3 anticorps Détail du antigène MAPK3 Information d'application Stockage Images Haut de la page
Background publications

Yoon, Seger: "The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions." dans: Growth factors (Chur, Switzerland), Vol. 24, Issue 1, pp. 21-44, 2006

Wolf: "Role of reactive oxygen species in angiotensin II-mediated renal growth, differentiation, and apoptosis." dans: Antioxidants & redox signaling, Vol. 7, Issue 9-10, pp. 1337-45, 2005

Boulton, Gregory, Cobb: "Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase." dans: Biochemistry, Vol. 30, Issue 1, pp. 278-86, 1991

Images

Détail du produit anti-MAPK3 anticorps Détail du antigène MAPK3 Information d'application Stockage References for anti-MAPK3 antibody (ABIN2486608) Haut de la page
Images (Fournisseur)
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal A...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Western blot analysis of Human Cell line lysates showing detection of ERK1 protein us...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: In...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ERK1 Polyclonal Ant...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: ba...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal A...