anti-Rat (Rattus) p65 anticorps pour Enzyme Immunoassay

Recommended p65 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Nuclear Factor-KB P65 (NFkBP65) Anticorps
  • C-Rel
  • NFKB3
  • p65
  • NFkB
  • c-rel
  • zgc:100833
  • REL proto-oncogene, NF-kB subunit
  • RELA proto-oncogene, NF-kB subunit
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel avian reticuloendotheliosis viral oncogene homolog
  • REL
  • RELA
  • Rela
  • Rel
  • rel
Épitope
Ser536
130
84
67
63
49
44
40
39
38
37
26
26
24
18
17
16
15
15
13
13
13
12
11
10
9
8
7
6
6
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
1138
655
508
72
56
55
30
25
25
23
13
8
8
6
4
2
1
1
Hôte
Lapin
993
133
17
11
1
Clonalité
Polyclonal
Conjugué
Cet anticorp p65 est non-conjugé
30
29
23
20
18
18
17
13
12
12
12
8
8
8
8
8
8
8
8
4
4
3
3
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
835
430
325
257
199
163
105
90
88
28
18
18
15
12
12
6
6
6
5
5
4
2
2
2
1
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N° du produit ABIN5551725
$ 636.30
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
1 ABIN318869 EIA IHC (p) WB Rabbit IgG Ser536 Connectez-vous pour afficher Polyclonal 1
1 ABIN5556626 EMSA EIA IP WB Rabbit C-Term Connectez-vous pour afficher Polyclonal 0
1 ABIN303222 EIA IHC (p) WB Rabbit Ser536 Connectez-vous pour afficher Polyclonal 0
1 ABIN303336 EIA IHC (p) IP WB Rabbit pSer529 Connectez-vous pour afficher Polyclonal 0

General

Antigène Nuclear Factor-KB P65 (NFkBP65) Anticorps
Épitope Ser536
(130), (84), (67), (63), (49), (44), (40), (39), (38), (37), (26), (26), (24), (18), (17), (16), (15), (15), (13), (13), (13), (12), (11), (10), (9), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Souris, Rat (Rattus)
(1138), (655), (508), (72), (56), (55), (30), (25), (25), (23), (13), (8), (8), (6), (4), (2), (1), (1)
Hôte Lapin
(993), (133), (17), (11), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp p65 est non-conjugé
(30), (29), (23), (20), (18), (18), (17), (13), (12), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(835), (430), (325), (257), (199), (163), (105), (90), (88), (28), (18), (18), (15), (12), (12), (6), (6), (6), (5), (5), (4), (2), (2), (2), (1)
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Détail du produit anti-p65 anticorps

Détail du antigène p65 Information d'application Stockage Images
Specificité This phosphospecific polyclonal antibody reacts with p65 (RelA) pS536 and shows minimal reactivity by western blot with nonphosphorylated p65 (RelA) and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Réactivité croisée (Details) Species reactivity (expected):Mouse, Rat (100 %)Species reactivity (tested):Human.
Attributs du produit Concentration: 0.9 mg/mL (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide
Immunogène Synthetic peptide corresponding to residues surrounding S536 of human p65 (RelA) protein
Isotype IgG

Détail du antigène p65

Détail du produit anti-p65 anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation RELA / NF-kB p65 (NFkBP65 Antibody Extrait)
Sujet NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), cRel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. Lowlevels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaB-alpha. IkappaB-alpha binds to the p65 subunit, preventing nuclear localization, and DNA binding. Activators mediate a rapid phosphorylation of IkappaB by IkappaB kinase (IKK), which results in subsequent ubiquitination and proteolytic degradation. NFkappaB is then transported to the nucleus, where it activates transcription of target genes through binding to NFkappaB target sequences within the promoter. The HTLV-I protein Tax can induce constitutive NFkappaB activation through phosphorylation of both IkappaB-alpha and IkappaB-beta. The transforming protein Tax inhibits p53 transcriptional activity through the NFkappaB signaling pathway, specifically via the p65 (RelA) subunit. The inhibition of p53 activity is dependent upon phosphorylation of p65 (RelA) at S536 by the upstream kinase IKKbeta.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
ID gène 5970
NCBI Accession NP_001138610
UniProt Q04206
Pathways Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Information d'application

Détail du produit anti-p65 anticorps Détail du antigène p65 Stockage Images Haut de la page
Indications d'application ELISA: 1: 1,000 - 1: 6,000. Western blot: 1: 200 - 1: 2,000 (a band approximately 65 kDa in size corresponding tophosphorylated p65 (RelA) protein is expected in the appropriate cell lysate or extract). Immunohistochemistry on paraffin sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Stockage

Détail du produit anti-p65 anticorps Détail du antigène p65 Information d'application Images Haut de la page
Concentration 0.9 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Stock 4 °C/-20 °C
Stockage commentaire Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.