anti-Humain ATM anticorps pour Immunofluorescence

Recommended ATM Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Ataxia Telangiectasia Mutated (ATM) Anticorps
  • ATM
  • Atm
  • CG6535
  • Dmel\\CG6535
  • Tefu
  • atm
  • atm/tefu
  • dATM
  • tef
  • Xatm
  • at1
  • atdc
  • tel1
  • telo1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • ATE
  • TEL1
  • TELO1
  • AI256621
  • C030026E19Rik
  • telomere fusion
  • ATM serine/threonine kinase L homeolog
  • ATM serine/threonine kinase
  • ataxia telangiectasia mutated
  • ataxia telangiectasia mutated (atm)
  • serine/threonine-protein kinase ATM
  • tefu
  • atm.L
  • atm
  • ATM
  • EDI_100660
  • CpipJ_CPIJ001772
  • BDBG_08252
  • PAAG_02532
  • MCYG_05088
  • VDBG_06833
  • ACLA_015700
  • LOC5565620
  • MGYG_07634
  • PGTG_14279
  • Atm
Épitope
C-Term
89
39
17
16
16
15
8
8
6
6
6
6
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris
357
157
78
27
8
1
1
1
1
Hôte
Lapin
230
100
17
9
3
Clonalité
Polyclonal
Conjugué
Cet anticorp ATM est non-conjugé
12
11
10
9
8
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
221
125
110
86
85
69
58
43
27
22
13
6
4
4
2
2
2
1
1
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N° du produit ABIN151030
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
11.745464 ABIN151622 ICC IF IHC IP WB Mouse IgG1 Connectez-vous pour afficher 5C2 26
11.745464 ABIN2668604 ICC IF Mouse IgG1 kappa pSer1981 Connectez-vous pour afficher 10H11-E-12 3
11.745464 ABIN1869997 IF IHC WB Rabbit IgG pSer1981 Connectez-vous pour afficher Polyclonal 0
11.745464 ABIN389888 DB IF Rabbit Ig Fraction pSer1981 Connectez-vous pour afficher Polyclonal 2
11.745464 ABIN513576 IF ELISA Mouse IgG2a kappa AA 1-138, full length Connectez-vous pour afficher 2C10 0
11.745464 ABIN5075177 ICC IF Rabbit IgG Connectez-vous pour afficher Polyclonal 0
11.745464 ABIN3030033 DB IF ELISA Rabbit Ig Fraction pSer1981 Connectez-vous pour afficher Polyclonal 0
1 ABIN151775 ChIP ELISA FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 full length Connectez-vous pour afficher 2C1 114
1 ABIN151772 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa pSer1981 Connectez-vous pour afficher 10H11-E12 20
1 ABIN319313 IF WB Rabbit Ser1981 Connectez-vous pour afficher Polyclonal 4
1 ABIN152102 ICC IF IHC (p) IP IHC PLA WB Rabbit AA 2550-2600 Connectez-vous pour afficher Polyclonal 2
1 ABIN363380 ICC FACS IF IP IHC ELISA WB Rabbit C-Term Connectez-vous pour afficher Polyclonal 0
1 ABIN362751 IF WB Rabbit IgG AA 1979-1983 Connectez-vous pour afficher Polyclonal 5
1 ABIN1882297 IF WB Rabbit IgG Connectez-vous pour afficher Polyclonal 0
1 ABIN1721771 ICC IF IHC WB Mouse IgG1 kappa pSer1981 Connectez-vous pour afficher 10H11-E12 0
1 ABIN5689237 IF WB Rabbit AA 1979-1983 Connectez-vous pour afficher Polyclonal 0
1 ABIN4282187 ELISA FACS ICC IF APC Mouse IgG1 full length Connectez-vous pour afficher 2C1 0
1 ABIN5499237 IF IHC (p) IP WB Mouse IgG1 AA 2577-3056 Connectez-vous pour afficher 2C1 0
1 ABIN2882455 IF IHC WB Rabbit IgG pSer1981 Connectez-vous pour afficher Polyclonal 0
1 ABIN4282171 ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 kappa pSer1981 Connectez-vous pour afficher 10H11-E12 0

General

Antigène Ataxia Telangiectasia Mutated (ATM) Anticorps
Épitope C-Term
(89), (39), (17), (16), (16), (15), (8), (8), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Souris
(357), (157), (78), (27), (8), (1), (1), (1), (1)
Hôte Lapin
(230), (100), (17), (9), (3)
Clonalité
Polyclonal
Conjugué Cet anticorp ATM est non-conjugé
(12), (11), (10), (9), (8), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(221), (125), (110), (86), (85), (69), (58), (43), (27), (22), (13), (6), (4), (4), (2), (2), (2), (1), (1)
Pubmed 77 références disponible
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Détail du produit anti-ATM anticorps

Détail du antigène ATM Information d'application Stockage References for anti-ATM antibody (ABIN151030) Images
Purification Immunogen affinity purified
Immunogène A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [UniProt# Q13315]

Détail du antigène ATM

Détail du produit anti-ATM anticorps Information d'application Stockage References for anti-ATM antibody (ABIN151030) Images Haut de la page
Antigène
Autre désignation ATM (ATM Antibody Extrait)
Sujet Gene Symbol: ATM
Poids moléculaire Theoretical MW: 350 kDa
ID gène 472
UniProt Q13315
Pathways Signalisation p53, Apoptose, Réparation de l'ADN, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus

Information d'application

Détail du produit anti-ATM anticorps Détail du antigène ATM Stockage References for anti-ATM antibody (ABIN151030) Images Haut de la page
Indications d'application Western Blot 1:500-1:1000, Flow Cytometry, ELISA, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100This ATM antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, it detects a band at ~350 kDa, representing ATM. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Immunohistochemistry-FFPE sections protocol specific for ATM Antibody Immunohistochemistry- FFPE sectionsDeparaffinization
1. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.
. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.Quench Endogenous Peroxidase
. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation.
. Place slides in distilled water: 2 changes for 2 minutes each.Retrieve Epitopes
. Preheat Citrate
Restrictions For Research Use only

Stockage

Détail du produit anti-ATM anticorps Détail du antigène ATM Information d'application References for anti-ATM antibody (ABIN151030) Images Haut de la page
Format Liquid
Concentration 2.5 mg/mL
Buffer PBS and 50 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock -80 °C,-20 °C
Stockage commentaire Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-ATM antibody (ABIN151030)

Détail du produit anti-ATM anticorps Détail du antigène ATM Information d'application Stockage Images Haut de la page
Produit citée dans:

Murata, Hashimoto, Urushihara, Shiga, Takeda, Jingu, Hosoi: "Knockdown of AMPKα decreases ATM expression and increases radiosensitivity under hypoxia and nutrient starvation in an SV40-transformed human fibroblast cell line, LM217." dans: Biochemical and biophysical research communications, Vol. 495, Issue 4, pp. 2566-2572, 2018 Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Li, Li, Kong, Yan, Ahmad, Liu: "Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response." dans: Cancer research, Vol. 77, Issue 12, pp. 3169-3180, 2017 Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Wu, Wang, Chen, Chao, Chang, Chen, Hung: "Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity." dans: Stem cells translational medicine, Vol. 6, Issue 6, pp. 1504-1514, 2017 Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Qi, Qiu, Gu, Tian, Zhang: "ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts." dans: Scientific reports, Vol. 6, pp. 24700, 2016 (Échantillon (espèces): Human). Détails: Immunocytochemistry,Immunofluorescence

Nagy, Kalousi, Furst, Koch, Fischer, Soutoglou: "Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs." dans: PLoS genetics, Vol. 12, Issue 2, pp. e1005791, 2016 Méthode utilisée: Western Blotting (WB)

Parplys, Zhao, Sharma, Groesser, Liang, Maranon, Leung, Grundt, Dray, Idate, Østvold, Schild, Sung, Wiese: "NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability." dans: Nucleic acids research, Vol. 43, Issue 20, pp. 9817-34, 2015 (Échantillon (espèces): Human). Détails: Western Blotting

Murata, Uehara, Hosoi: "Activation of mTORC1 under nutrient starvation conditions increases cellular radiosensitivity in human liver cancer cell lines, HepG2 and HuH6." dans: Biochemical and biophysical research communications, Vol. 468, Issue 4, pp. 684-90, 2015 (Échantillon (espèces): Human). Détails: Western Blotting

Yoon, Ishdorj, Graham, Johnston, Gibson: "Valproic acid enhances fludarabine-induced apoptosis mediated by ROS and involving decreased AKT and ATM activation in B-cell-lymphoid neoplastic cells." dans: Apoptosis : an international journal on programmed cell death, Vol. 19, Issue 1, pp. 191-200, 2014 (Échantillon (espèces): Human). Détails: Western Blotting

Morita, Tanimoto, Murakami, Morinaga, Hosoi: "Mitochondria are required for ATM activation by extranuclear oxidative stress in cultured human hepatoblastoma cell line Hep G2 cells." dans: Biochemical and biophysical research communications, Vol. 443, Issue 4, pp. 1286-90, 2014 (Échantillon (espèces): Human). Détails: Western Blotting

Silva, Stambaugh, Yokomori, Shah, Berns: "DNA damage to a single chromosome end delays anaphase onset." dans: The Journal of biological chemistry, Vol. 289, Issue 33, pp. 22771-84, 2014

Brinley, Theriot, Nelman-Gonzalez, Crucian, Stowe, Barrett, Pierson: "Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system." dans: Journal of cellular biochemistry, Vol. 114, Issue 3, pp. 616-24, 2013 (Échantillon (espèces): Human). Détails: Flow Cytometry

Gautam, Bridge: "The kinase activity of ataxia-telangiectasia mutated interferes with adenovirus E4 mutant DNA replication." dans: Journal of virology, Vol. 87, Issue 15, pp. 8687-96, 2013 (Échantillon (espèces): Human). Détails: Western Blotting

Rankin, Sarkar, Faller: "Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells." dans: Journal of cellular physiology, Vol. 227, Issue 6, pp. 2586-94, 2012 (Échantillon (espèces): Human). Détails: Western Blotting

Jacquemin, Rieunier, Jacob, Bellanger, dEnghien, Laugé, Stoppa-Lyonnet, Stern: "Underexpression and abnormal localization of ATM products in ataxia telangiectasia patients bearing ATM missense mutations." dans: European journal of human genetics : EJHG, Vol. 20, Issue 3, pp. 305-12, 2012 (Échantillon (espèces): Human). Détails: Immunocytochemistry,Immunofluorescence

Nguyen, Possemato, Bauerlein, Xie, Scully, Hahn: "Nek4 regulates entry into replicative senescence and the response to DNA damage in human fibroblasts." dans: Molecular and cellular biology, Vol. 32, Issue 19, pp. 3963-77, 2012 (Échantillon (espèces): Human). Détails: Western Blotting

Xie, Li, Xing, Tian, Yin, He, Zhang: "ATM-mediated NuSAP phosphorylation induces mitotic arrest." dans: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 413-8, 2011 (Échantillon (espèces): Human). Détails: Western Blotting

Wang, Wu, Wei, Hong, Chiang: "Inactivation of ataxia telangiectasia mutated gene can increase intracellular reactive oxygen species levels and alter radiation-induced cell death pathways in human glioma cells." dans: International journal of radiation biology, Vol. 87, Issue 4, pp. 432-42, 2011 (Échantillon (espèces): Human). Détails: Flow Cytometry

Dixon, Henry, Siroky, Chu, Groen, Bissler: "Cell cycle control and DNA damage response of conditionally immortalized urothelial cells." dans: PLoS ONE, Vol. 6, Issue 1, pp. e16595, 2011 (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Du, Kayali, Bertoni, Fike, Hu, Iversen, Gatti: "Arginine-rich cell-penetrating peptide dramatically enhances AMO-mediated ATM aberrant splicing correction and enables delivery to brain and cerebellum." dans: Human molecular genetics, Vol. 20, Issue 16, pp. 3151-60, 2011 (Échantillon (espèces): Human). Détails: Western Blotting

Yang, Chou, Pan, Chen, Tsou, Yeh, Cheung, Chen, Chang: "Enhancement of non-homologous end joining DNA repair capacity confers cancer cells resistance to the novel selenophene compound, D-501036." dans: Cancer letters, Vol. 309, Issue 1, pp. 110-8, 2011 (Échantillon (espèces): Human). Détails: Western Blotting

Images

Détail du produit anti-ATM anticorps Détail du antigène ATM Information d'application Stockage References for anti-ATM antibody (ABIN151030) Haut de la page
Supplier Images
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in HeLa whole cell lysate ...
Immunofluorescence (IF) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunohistochemical staining using ABIN151030.
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Detection of ATM in HeLa whole cell lysate using ABIN151030. (30 second exposure)
Western Blotting (WB) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in Raji whole cell lysate.
Flow Cytometry (FACS) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Flow Cytometry: ATM Antibody [ABIN151030] - Analysis of PE conjugate of ABIN151030. A...
Immunohistochemistry (IHC) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunohistochemistry: ATM Antibody [ABIN151030] - Staining of Testis, Seminiferous Tu...
Immunofluorescence (IF) image for anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (ABIN151030) Immunocytochemistry/Immunofluorescence: ATM Antibody [ABIN151030] - HeLa cells were f...