anti-Hamster BAI1 anticorps pour Immunohistochemistry

Recommended BAI1 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Brain-Specific Angiogenesis Inhibitor 1 (BAI1) Anticorps
  • BAI1
  • GDAIF
  • B830018M07Rik
  • R75078
  • mKIAA4089
  • brain-specific angiogenesis inhibitor 1
  • adhesion G protein-coupled receptor B1
  • BAI1
  • ADGRB1
  • LOC100228084
  • Adgrb1
Épitope
C-Term
35
7
6
4
3
2
2
1
1
1
1
1
Reactivité
Poulet, Boeuf (Vache), Hamster, Humain, Singe, Souris, Rat (Rattus)
91
51
24
6
2
1
1
Hôte
Lapin
92
1
Clonalité
Polyclonal
Conjugué
Cet anticorp BAI1 est non-conjugé
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
43
39
32
29
13
11
5
2
2
1
1
1
Options
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N° du produit (Fournisseur)
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N° du produit ABIN1048337
$ 484.00
Plus shipping costs $45.00

General

Antigène Brain-Specific Angiogenesis Inhibitor 1 (BAI1) Anticorps
Épitope C-Term
(35), (7), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivité Poulet, Boeuf (Vache), Hamster, Humain, Singe, Souris, Rat (Rattus)
(91), (51), (24), (6), (2), (1), (1)
Hôte Lapin
(92), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp BAI1 est non-conjugé
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(43), (39), (32), (29), (13), (11), (5), (2), (2), (1), (1), (1)
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Détail du produit anti-BAI1 anticorps

Détail du antigène BAI1 Information d'application Stockage Images
Marque IHC-plus™
Specificité Human BAI1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Bovine, Opossum, Chicken, Platypus (100%) Xenopus, Zebrafish (94%) Stickleback, Pufferfish (88%).
Purification Immunoaffinity purified
Immunogène Synthetic 16 amino acid peptide from C-terminus of human BAI1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Bovine, Opossum, Chicken, Platypus (100%), Xenopus, Zebrafish (94%), Stickleback, Pufferfish (88%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Détail du antigène BAI1

Détail du produit anti-BAI1 anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation ADGRB1 / BAI1 (BAI1 Antibody Extrait)
Sujet Name/Gene ID: ADGRB1
Subfamily: Orphan-B
Family: GPCR

Synonyms: ADGRB1, BAI1, GDAIF
ID gène 575
Pathways Signalisation p53

Information d'application

Détail du produit anti-BAI1 anticorps Détail du antigène BAI1 Stockage Images Haut de la page
Indications d'application Approved: IHC, IHC-P (14 μg/mL)
Commentaires

Target Species of Antibody: Human

Procédure de l'essai The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Stockage

Détail du produit anti-BAI1 anticorps Détail du antigène BAI1 Information d'application Images Haut de la page
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C,-20 °C
Stockage commentaire Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Date de péremption 12 months

Images

Détail du produit anti-BAI1 anticorps Détail du antigène BAI1 Information d'application Stockage Haut de la page
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Brain-Specific Angiogenesis Inhibitor 1 (BAI1) (C-Term) antibody (ABIN1048337) Anti-BAI1 antibody ABIN1048337 IHC staining of human brain, neurons. Immunohistochem...
Immunohistochemistry (IHC) image for anti-Brain-Specific Angiogenesis Inhibitor 1 (BAI1) (C-Term) antibody (ABIN1048337) Anti-BAI1 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formali...