anti-Boeuf (Vache) PAG1 anticorps pour Western Blotting

Recommended PAG1 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Anticorps
  • Cbp
  • F730007C19Rik
  • Pag
  • CBP
  • PAG
  • MGC80192
  • phosphoprotein associated with glycosphingolipid microdomains 1
  • phosphoprotein membrane anchor with glycosphingolipid microdomains 1
  • phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog
  • Pag1
  • PAG1
  • pag1.L
  • pag1
Épitope
C-Term
32
25
16
4
3
2
2
2
1
1
Reactivité
Humain, Souris, Rat (Rattus), Boeuf (Vache)
100
62
46
28
2
2
1
1
1
Hôte
Souris
54
54
Clonalité
Monoclonal
Conjugué
Cet anticorp PAG1 est non-conjugé
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
69
55
47
31
23
13
11
6
3
1
1
Options
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N° du produit ABIN94270
$ 436.43
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
7 ABIN351516 IHC IHC (p) IP WB Mouse IgG2b C-Term Connectez-vous pour afficher PAG-C1 0
4.802582 ABIN2789626 WB Rabbit C-Term Connectez-vous pour afficher Polyclonal 0
1 ABIN3200740 IHC (p) IP WB Mouse IgG2b C-Term Connectez-vous pour afficher PAG-C1 0
1 ABIN6106285 ELISA WB Rabbit IgG AA 54-380 Connectez-vous pour afficher Polyclonal 0

General

Antigène phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Anticorps
Épitope C-Term
(32), (25), (16), (4), (3), (2), (2), (2), (1), (1)
Reactivité Humain, Souris, Rat (Rattus), Boeuf (Vache)
(100), (62), (46), (28), (2), (2), (1), (1), (1)
Hôte Souris
(54), (54)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp PAG1 est non-conjugé
(4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(69), (55), (47), (31), (23), (13), (11), (6), (3), (1), (1)
Pubmed 5 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-PAG1 anticorps

Détail du antigène PAG1 Information d'application Stockage References for anti-PAG1 antibody (ABIN94270) Images
Specificité The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Purification Purified from ascites by protein-A affinity chromatography.
Pureté > 95 % (by SDS-PAGE)
Immunogène C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Clone PAG-C1
Isotype IgG2b
Plasmids, Primers & others

Détail du antigène PAG1

Détail du produit anti-PAG1 anticorps Information d'application Stockage References for anti-PAG1 antibody (ABIN94270) Images Haut de la page
Antigène
Autre désignation PAG1 (PAG1 Antibody Extrait)
Sujet PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Pathways Signalisation p53, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events

Information d'application

Détail du produit anti-PAG1 anticorps Détail du antigène PAG1 Stockage References for anti-PAG1 antibody (ABIN94270) Images Haut de la page
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Stockage

Détail du produit anti-PAG1 anticorps Détail du antigène PAG1 Information d'application References for anti-PAG1 antibody (ABIN94270) Images Haut de la page
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Stock 4 °C
Stockage commentaire Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-PAG1 antibody (ABIN94270)

Détail du produit anti-PAG1 anticorps Détail du antigène PAG1 Information d'application Stockage Images Haut de la page
Background publications

Simeoni, Smida, Posevitz, Schraven, Lindquist: "Right time, right place: the organization of membrane proximal signaling." dans: Seminars in immunology, Vol. 17, Issue 1, pp. 35-49, 2004

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." dans: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004

Baumgartner, Angelisová, Setterblad, Mooney, Werling, Horejsí, Langsley: "Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes." dans: Blood, Vol. 101, Issue 5, pp. 1874-81, 2003

Produit citée dans:

Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." dans: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003

Vang, Abrahamsen, Myklebust, Horejsí, Taskén: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." dans: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003

Images

Détail du produit anti-PAG1 anticorps Détail du antigène PAG1 Information d'application Stockage References for anti-PAG1 antibody (ABIN94270) Haut de la page
Images (Fournisseur)
Immunoprecipitation (IP) image for anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody (ABIN94270) Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma c...
Immunohistochemistry (IHC) image for anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody (ABIN94270) Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of ly...