anti-Humain IGF1R anticorps pour Western Blotting

Recommended IGF1R Antibody

Antigène
Insulin-Like Growth Factor 1 Receptor (IGF1R) Anticorps
  • CD221
  • IGFIR
  • IGFR
  • JTK13
  • IGFIRC
  • IGFR1
  • IGF-1R
  • IGF1R
  • DKFZp469I0618
  • igf-1r
  • xIGF-1R
  • xIGFR
  • xigf1r
  • A330103N21Rik
  • D930020L01
  • hyft
  • igf-ir
  • IGF-IRa
  • sb:eu1090
  • wu:fi20b10
  • insulin like growth factor 1 receptor
  • insulin-like growth factor 1 receptor
  • insulin-like growth factor I receptor
  • insulin like growth factor 1 receptor S homeolog
  • insulin-like growth factor 1a receptor
  • IGF1R
  • Igf1r
  • igf1r
  • LOC780503
  • LOC100136427
  • igf1r.S
  • igf1ra
Épitope
AA 260-290
52
45
40
36
33
29
24
21
17
15
15
15
15
15
9
9
8
7
7
7
7
6
5
5
5
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
565
319
302
21
19
18
12
11
6
4
4
3
2
2
1
Hôte
Lapin
466
98
2
2
1
Clonalité
Polyclonal
Conjugué
Cet anticorp IGF1R est non-conjugé
23
19
16
15
14
14
14
14
14
14
8
8
8
8
8
7
6
2
Application
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
358
166
152
103
89
67
61
49
15
13
8
6
3
3
2
1
1
Options
Testé avec succès

Images

N° du produit ABIN726575
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
28.059069 ABIN969207 IHC ELISA WB Mouse IgG2a AA 1101-1367 3G5C1 2
23.559069 ABIN932245 IHC ELISA WB Mouse IgG2a 3G5C1 0
23.559069 ABIN3020253 IF IHC WB Rabbit pTyr1161 Polyclonal 0
22.059069 ABIN3020768 WB Rabbit IgG N-Term Polyclonal 2
20.559069 ABIN3182037 ELISA IHC IP WB Rabbit IgG pTyr1161 Polyclonal 0
20.559069 ABIN3182038 ELISA WB Rabbit IgG pTyr1165, pTyr1166 Polyclonal 0
20.559069 ABIN3185133 ELISA IHC WB Rabbit IgG Ser1026 Polyclonal 0
20.559069 ABIN926846 IHC WB Rabbit Middle Region Polyclonal 0
20.559069 ABIN932527 IHC ELISA WB Mouse IgG2b 3C8B1 0
20.559069 ABIN3019659 IF WB Rabbit pTyr1165, pTyr1166 Polyclonal 0
19.857681 ABIN6256637 ELISA ICC IF IHC WB Rabbit IgG pTyr1346 Polyclonal 0
19.857681 ABIN3031345 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 51-77 Polyclonal 0
19.151108 ABIN259940 ELISA IHC IHC (p) WB Mouse IgG2a 3G5C1 10
18.357681 ABIN6255780 ELISA ICC IF IHC WB Rabbit IgG pTyr1165, pTyr1166 Polyclonal 1
18.201385 ABIN966348 IHC ELISA WB IgG2b 2
17.559069 ABIN3185141 ELISA IHC WB Rabbit IgG Ser528 Polyclonal 0
17.559069 ABIN3182743 ELISA IF IHC WB Rabbit IgG pTyr1161 Polyclonal 0
17.559069 ABIN3185134 ELISA WB Rabbit IgG Ser52 Polyclonal 0
17.559069 ABIN3020256 WB Rabbit pTyr1280 Polyclonal 0
16.857681 ABIN3044138 IHC (p) WB Rabbit IgG AA 1353-1367, C-Term Polyclonal 8

General

Antigène Insulin-Like Growth Factor 1 Receptor (IGF1R) Anticorps
Épitope AA 260-290
(52), (45), (40), (36), (33), (29), (24), (21), (17), (15), (15), (15), (15), (15), (9), (9), (8), (7), (7), (7), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Souris, Rat (Rattus)
(565), (319), (302), (21), (19), (18), (12), (11), (6), (4), (4), (3), (2), (2), (1)
Hôte Lapin
(466), (98), (2), (2), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp IGF1R est non-conjugé
(23), (19), (16), (15), (14), (14), (14), (14), (14), (14), (8), (8), (8), (8), (8), (7), (6), (2)
Application Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(358), (166), (152), (103), (89), (67), (61), (49), (15), (13), (8), (6), (3), (3), (2), (1), (1)
Pubmed 2 références disponible
Fournisseur

Détail du produit anti-IGF1R anticorps

Détail du antigène IGF1R Information d'application Stockage References for anti-IGF1R antibody (ABIN726575) Images
Purification Purified by Protein A.
Immunogène KLH conjugated synthetic peptide derived from human IGF-1R alpha chain
Isotype IgG
Plasmids, Primers & others

Détail du antigène IGF1R

Détail du produit anti-IGF1R anticorps Information d'application Stockage References for anti-IGF1R antibody (ABIN726575) Images Haut de la page
Antigène
Autre désignation IGF1R (IGF1R Antibody Extrait)
Sujet

When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

Subcellular location: Extracellular

Synonyms: IGFR, CD221, IGFIR, JTK13, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, IGF1R

ID gène 3480
UniProt P08069
Pathways Signalisation RTK, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy

Information d'application

Détail du produit anti-IGF1R anticorps Détail du antigène IGF1R Stockage References for anti-IGF1R antibody (ABIN726575) Images Haut de la page
Indications d'application WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Stockage

Détail du produit anti-IGF1R anticorps Détail du antigène IGF1R Information d'application References for anti-IGF1R antibody (ABIN726575) Images Haut de la page
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20°C
Date de péremption 12 months

References for anti-IGF1R antibody (ABIN726575)

Détail du produit anti-IGF1R anticorps Détail du antigène IGF1R Information d'application Stockage Images Haut de la page
Produit citée dans:

Hu, Li, Hu, Wu, Li, Meng: "MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene." dans: Growth factors (Chur, Switzerland), Vol. 32, Issue 1, pp. 27-33, 2014 Méthode utilisée: Western Blotting (WB)

Yang, Liu, Li, Wen, Zhu, Xu: "Insulin-Like Growth Factor-1 Modulates Polycomb Cbx8 Expression and Inhibits Colon Cancer Cell Apoptosis." dans: Cell biochemistry and biophysics, 2014 Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Images

Détail du produit anti-IGF1R anticorps Détail du antigène IGF1R Information d'application Stockage References for anti-IGF1R antibody (ABIN726575) Haut de la page
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Formalin-fixed and paraffin-embedded human lung carcinoma tissue labeled with Rabbit ...
Immunohistochemistry (IHC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-IGF1R/CD...
Flow Cytometry (FACS) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody .
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-IGF1R/CD...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Formalin-fixed and paraffin-embedded human lung carcinoma tissue labeled with Rabbit ...
'Independent Validation' signe
Numéro du lot 130322
Application validée Western Blotting
Contrôle positif MCF-7 cells – high expression
Contrôle négative PC3 cells – low expression
Conclusion Two strong bands were observed in the positive control, one at the expected molecular weight, and one slightly higher (which may represent a glycosylated or precursor form). Weaker bands of the same molecular weight appear in the negative control, which is consistent with the expected lower expression in PC3 cells. No other major bands are present in either positive or negative controls.
Anticorps primaire
  • Antigen: Insulin-Like Growth Factor 1 Receptor (IGF1R) (1:200 dilution)
  • Catalog number: ABIN726575
  • Lot number: 130322
Anticorps secondaire
  • Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
  • Lot number: L170-6515
Contrôles
  • Positive control: MCF-7 cell extract
  • Negative control: PC-3 cell extract
Protocole
  • Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 46 μg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
  • The Spectra Multicolor Broad Range (Thermo Scientific, Cat # 26634) were used as molecular mass markers.
  • Proteins were transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
  • The membrane was rinsed with TBST once.
  • The membrane was immersed with the protein side up in the primary antibody solution (anti-IGF1R; 1:200) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 hours at 4°C.
  • The membrane was rinsed in TBST three times for 5 min each.
  • The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • The membrane was rinsed three times with TBST for 5 min each.
  • The membrane was rinsed in TBS twice for 30 s each.
  • Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • The membrane was rinsed three times with TBST, then incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • The membrane was washed in TBST 2 times for 10 min each.
  • Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Notes - No challenges noted.
Validation Images
Western Blotting validation image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Figure 1. Western blot of lysates from MCF7 cells (Lane 1) and PC-3 cells (Lane 2) pr...