anti-Souris KIT anticorps pour Blocking Reagent

Recommended KIT Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Mast/stem Cell Growth Factor Receptor (KIT) Anticorps
  • C-Kit
  • CD117
  • PBT
  • SCFR
  • Bs
  • Fdc
  • Gsfsco1
  • Gsfsco5
  • Gsfsow3
  • SCO1
  • SCO5
  • SOW3
  • Ssm
  • Tr-kit
  • W
  • c-KIT
  • c-kit
  • MGF
  • KIT
  • C-KIT
  • kit
  • receptor
  • sparse
  • Xkl-1
  • kit-a
  • kit-b
  • kl1-A
  • krk1
  • pbt
  • scfr
  • KIT proto-oncogene receptor tyrosine kinase
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a
  • KIT proto-oncogene receptor tyrosine kinase S homeolog
  • KIT
  • Kit
  • kita
  • kit
  • kit.S
Reactivité
Souris
1430
594
173
45
45
38
26
22
14
5
4
4
4
4
3
1
1
1
Hôte
Rat
1062
439
274
18
2
2
Clonalité
Monoclonal
Conjugué
Cet anticorp KIT est non-conjugé
156
142
135
118
90
76
22
18
15
13
10
10
10
10
9
8
8
8
8
8
8
7
7
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
2
2
2
Application
Blocking Reagent (BR), Flow Cytometry (FACS)
705
699
576
363
312
175
166
124
105
84
68
39
19
13
12
10
8
6
5
3
3
3
3
3
3
2
2
2
2
1
1
Options
Fournisseur
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N° du produit (Fournisseur)
Connectez-vous pour afficher
N° du produit ABIN2688893
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
14.5 ABIN1176965 BR FACS Rat IgG2b, kappa Connectez-vous pour afficher ACK45 20
4 ABIN2664146 BR FACS IP ELISA WB Rat IgG2b kappa Connectez-vous pour afficher ACK2 6
1 ABIN2664145 BR FACS IP ELISA WB Rat IgG2b kappa Connectez-vous pour afficher ACK2 6

General

Antigène Mast/stem Cell Growth Factor Receptor (KIT) Anticorps
Reactivité Souris
(1430), (594), (173), (45), (45), (38), (26), (22), (14), (5), (4), (4), (4), (4), (3), (1), (1), (1)
Hôte Rat
(1062), (439), (274), (18), (2), (2)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp KIT est non-conjugé
(156), (142), (135), (118), (90), (76), (22), (18), (15), (13), (10), (10), (10), (10), (9), (8), (8), (8), (8), (8), (8), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2)
Application Blocking Reagent (BR), Flow Cytometry (FACS)
(705), (699), (576), (363), (312), (175), (166), (124), (105), (84), (68), (39), (19), (13), (12), (10), (8), (6), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Pubmed 20 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit anti-KIT anticorps

Détail du antigène KIT Information d'application Stockage References for anti-KIT antibody (ABIN2688893) Images
Marque BD Pharmingen™
Attributs du produit The ACK45 antibody reacts with CD117 (c-Kit), a transmembrane tyrosine-kinase receptor which is encoded by the Kit gene (formerly dominant white spotting, W). The c-Kit ligand (also known as steel factor, stem cell factor, and mast-cell growth factor) encoded by the Kitl gene (formerly steel, Sl), is a co-mitogen for hematopoietic stem cells and the myeloerythroid progenitors and a mast-cell differentiation factor. The Kit[W] and Kitl[Sl] mutant alleles have similar pleiotropic effects on the development of melanocytes, germ cells, and the hematopoietic system. In the adult bone marrow, CD117 is expressed on hematopoietic progenitor cells, including the CD90 (Thy-1)[lo] TER-119[-] CD45R/B220[-] CD11b (Mac-1)[-] Ly-6G (Gr-1)[-] CD4[-] CD8[-] Sca-1 (Ly-6A/E)[+] multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and precursors of B and T lymphocytes. This widespread expression of CD117 in hematopoietic precursors is consistent with the participation of c-Kit and its ligand in the regulation of several hematopoietic lineages. Intrathymic expression of c-Kit and c-Kit ligand suggest that CD117 is also involved in the regulation of some events during the development of T lymphocytes. CD117 is also expressed by mast cells. The expression of CD117 has been included in protocols to isolate hematopoietic progenitors from fetal and adult peripheral blood. The specificity and functional activity of mAb ACK45 is very similar to that of the published antibody ACK22, it is reported to block the action of c-Kit in vitro and in vivo. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD117 on mouse bone marrow cells. A single-cell suspension of BALB/c bone marrow was simultaneously stained with FITC-conjugated RA3-6B2 (anti-mouse CD45R/B220, Cat. No. 553087/553088, both panels) and purified ACK45 (right panel) monoclonal antibodies, followed by biotinylated anti-rat IgG2b mAb RG7/11.1 (Cat. No. 553898, both panels) then Streptavidin-PE (Cat. No. 554061, both panels). Flow cytometry was performed on a FACScan™ . 553868 Rev. 6 Page 1 of 2

BD Pharmingen™ Purified Rat Anti-Mouse CD117 - Purified - Clone ACK45 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogène Mouse bone marrow mast cells
Clone ACK45
Isotype IgG2b, kappa
Plasmids, Primers & others

Détail du antigène KIT

Détail du produit anti-KIT anticorps Information d'application Stockage References for anti-KIT antibody (ABIN2688893) Images Haut de la page
Antigène
Autre désignation CD117 (KIT Antibody Extrait)
Sujet Synonyms: c-Kit
Pathways Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity

Information d'application

Détail du produit anti-KIT anticorps Détail du antigène KIT Stockage References for anti-KIT antibody (ABIN2688893) Images Haut de la page
Indications d'application Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Stockage

Détail du produit anti-KIT anticorps Détail du antigène KIT Information d'application References for anti-KIT antibody (ABIN2688893) Images Haut de la page
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Stockage commentaire Store undiluted at 4°C.

References for anti-KIT antibody (ABIN2688893)

Détail du produit anti-KIT anticorps Détail du antigène KIT Information d'application Stockage Images Haut de la page
Produit citée dans:

Austen, Boyce: "Mast cell lineage development and phenotypic regulation." dans: Leukemia research, Vol. 25, Issue 7, pp. 511-8, 2001

Domen, Weissman: "Hematopoietic stem cells need two signals to prevent apoptosis; BCL-2 can provide one of these, Kitl/c-Kit signaling the other." dans: The Journal of experimental medicine, Vol. 192, Issue 12, pp. 1707-18, 2001

Mirmonsef, Shelburne, Fitzhugh Yeatman, Chong, Ryan: "Inhibition of Kit expression by IL-4 and IL-10 in murine mast cells: role of STAT6 and phosphatidylinositol 3'-kinase." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2530-9, 1999

Rodewald, Dessing, Dvorak, Galli: "Identification of a committed precursor for the mast cell lineage." dans: Science (New York, N.Y.), Vol. 271, Issue 5250, pp. 818-22, 1996

Yamamoto, Yasumizu, Amou, Watanabe, Nishio, Toki, Fukuhara, Ikehara: "Characterization of peripheral blood stem cells in mice." dans: Blood, Vol. 88, Issue 2, pp. 445-54, 1996

Rodewald, Kretzschmar, Swat, Takeda: "Intrathymically expressed c-kit ligand (stem cell factor) is a major factor driving expansion of very immature thymocytes in vivo." dans: Immunity, Vol. 3, Issue 3, pp. 313-9, 1995

Godfrey, Kennedy, Mombaerts, Tonegawa, Zlotnik: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, 1994

Moore, Huang, Terstappen, Bennett, Kumar: "Expression of CD43 on murine and human pluripotent hematopoietic stem cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 4978-87, 1994

Rico-Vargas, Weiskopf, Nishikawa, Osmond: "c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2845-52, 1994

Rodewald, Kretzschmar, Takeda, Hohl, Dessing: "Identification of pro-thymocytes in murine fetal blood: T lineage commitment can precede thymus colonization." dans: The EMBO journal, Vol. 13, Issue 18, pp. 4229-40, 1994

Valent: "The riddle of the mast cell: kit(CD117)-ligand as the missing link?" dans: Immunology today, Vol. 15, Issue 3, pp. 111-4, 1994

Katayama, Shih, Nishikawa, Kina, Clark, Ogawa: "Stage-specific expression of c-kit protein by murine hematopoietic progenitors." dans: Blood, Vol. 82, Issue 8, pp. 2353-60, 1993

Matsuzaki, Gyotoku, Ogawa, Nishikawa, Katsura, Gachelin, Nakauchi: "Characterization of c-kit positive intrathymic stem cells that are restricted to lymphoid differentiation." dans: The Journal of experimental medicine, Vol. 178, Issue 4, pp. 1283-92, 1993

Orlic, Fischer, Nishikawa, Nienhuis, Bodine: "Purification and characterization of heterogeneous pluripotent hematopoietic stem cell populations expressing high levels of c-kit receptor." dans: Blood, Vol. 82, Issue 3, pp. 762-70, 1993

Godfrey, Zlotnik, Suda: "Phenotypic and functional characterization of c-kit expression during intrathymic T cell development." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2281-5, 1992

Nishikawa, Kusakabe, Yoshinaga, Ogawa, Hayashi, Kunisada, Era, Sakakura, Nishikawa: "In utero manipulation of coat color formation by a monoclonal anti-c-kit antibody: two distinct waves of c-kit-dependency during melanocyte development." dans: The EMBO journal, Vol. 10, Issue 8, pp. 2111-8, 1991

Ogawa, Matsuzaki, Nishikawa, Hayashi, Kunisada, Sudo, Kina, Nakauchi, Nishikawa: "Expression and function of c-kit in hemopoietic progenitor cells." dans: The Journal of experimental medicine, Vol. 174, Issue 1, pp. 63-71, 1991

Anderson, Lyman, Baird, Wignall, Eisenman, Rauch, March, Boswell, Gimpel, Cosman: "Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms." dans: Cell, Vol. 63, Issue 1, pp. 235-43, 1990

Huang, Nocka, Beier, Chu, Buck, Lahm, Wellner, Leder, Besmer: "The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus." dans: Cell, Vol. 63, Issue 1, pp. 225-33, 1990

Zsebo, Williams, Geissler, Broudy, Martin, Atkins, Hsu, Birkett, Okino, Murdock: "Stem cell factor is encoded at the Sl locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor." dans: Cell, Vol. 63, Issue 1, pp. 213-24, 1990