anti-Souris TEK anticorps pour Immunocytochemistry

Recommended TEK Antibody

Antigène
TEK Tyrosine Kinase, Endothelial (TEK) Anticorps
  • TEK
  • DKFZp469E064
  • AA517024
  • Cd202b
  • Hyk
  • Tie2
  • tie-2
  • CD202B
  • TIE-2
  • TIE2
  • VMCM
  • VMCM1
  • Tie-2
  • tie2
  • TEK receptor tyrosine kinase
  • angiopoietin-1 receptor
  • TEK tyrosine kinase, endothelial
  • TEK
  • LOC100408334
  • tek
  • Tek
Épitope
pSer1119
29
25
22
20
16
15
11
10
9
8
8
8
6
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris
288
163
101
9
8
Hôte
Lapin
236
64
10
3
2
1
Clonalité
Polyclonal
Conjugué
Cet anticorp TEK est non-conjugé
21
14
10
8
8
8
8
8
8
7
6
4
4
4
4
4
4
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
173
127
69
53
48
22
21
18
16
12
8
6
5
3
Options

Images

N° du produit ABIN6271552
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN1863206 ICC IHC WB Rabbit IgG AA 44-341 Polyclonal 0

General

Antigène TEK Tyrosine Kinase, Endothelial (TEK) Anticorps
Épitope pSer1119
(29), (25), (22), (20), (16), (15), (11), (10), (9), (8), (8), (8), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Souris
(288), (163), (101), (9), (8)
Hôte Lapin
(236), (64), (10), (3), (2), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp TEK est non-conjugé
(21), (14), (10), (8), (8), (8), (8), (8), (8), (7), (6), (4), (4), (4), (4), (4), (4), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(173), (127), (69), (53), (48), (22), (21), (18), (16), (12), (8), (6), (5), (3)
Fournisseur

Détail du produit anti-TEK anticorps

Détail du antigène TEK Information d'application Stockage Images
Specificité TIE2 (Phospho-Ser1119) Antibody detects endogenous levels of TIE2 only when phosphorylated at Ser1119
 Réactivité croisée Humain, Souris
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogène A synthesized peptide derived from human TIE2 (Phospho-Ser1119)
Isotype IgG
Plasmids, Primers & others

Détail du antigène TEK

Détail du produit anti-TEK anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation TIE2 (TEK Antibody Extrait)
Sujet

Description: Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1 and TIE1.

Gene: TEK

Poids moléculaire 126kDa
ID gène 7010
UniProt Q02763
Pathways Signalisation RTK, Growth Factor Binding

Information d'application

Détail du produit anti-TEK anticorps Détail du antigène TEK Stockage Images Haut de la page
Indications d'application WB 1:1000-3000, IF/ICC 1:100-1:500
Restrictions For Research Use only

Stockage

Détail du produit anti-TEK anticorps Détail du antigène TEK Information d'application Images Haut de la page
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store at -20 °C.Stable for 12 months from date of receipt
Date de péremption 12 months

Images

Détail du produit anti-TEK anticorps Détail du antigène TEK Information d'application Stockage Haut de la page
Images (Fournisseur)
Immunofluorescence (fixed cells) (IF/ICC) image for anti-TEK Tyrosine Kinase, Endothelial (TEK) (pSer1119) antibody (ABIN6271552) ABIN6271553 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabili...
Western Blotting (WB) image for anti-TEK Tyrosine Kinase, Endothelial (TEK) (pSer1119) antibody (ABIN6271552) Western blot analysis of TIE2 (Phospho-Ser1119) using EGF treated 293 whole cell lysates