anti-Souris TEK anticorps pour Intracellular Staining

Recommended TEK Antibody

Antigène
TEK Tyrosine Kinase, Endothelial (TEK) Anticorps
  • TEK
  • DKFZp469E064
  • AA517024
  • Cd202b
  • Hyk
  • Tie2
  • tie-2
  • CD202B
  • TIE-2
  • TIE2
  • VMCM
  • VMCM1
  • Tie-2
  • tie2
  • TEK receptor tyrosine kinase
  • angiopoietin-1 receptor
  • TEK tyrosine kinase, endothelial
  • TEK
  • LOC100408334
  • tek
  • Tek
Épitope
pTyr992
29
25
22
20
16
15
11
10
10
8
8
8
6
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris
289
164
101
9
8
Hôte
Souris
237
64
10
3
2
1
Clonalité
Monoclonal
Conjugué
Cet anticorp TEK est conjugé à/à la Alexa Fluor 647
21
14
10
8
8
8
8
8
8
7
6
4
4
4
4
4
4
1
Application
Intracellular Staining (ICS)
174
128
69
53
48
22
21
19
16
12
8
6
6
3
Options
N° du produit ABIN1177217
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
10 ABIN1177218 ICS PE Mouse IgG2a, kappa pTyr1102 K93-754 6
10 ABIN1177219 ICS Alexa Fluor 647 Mouse IgG2a, kappa pTyr1102 K93-754 6
10 ABIN1177220 ICS Alexa Fluor 488 Mouse IgG2a, kappa pTyr1102 K93-754 6

General

Antigène TEK Tyrosine Kinase, Endothelial (TEK) Anticorps
Épitope pTyr992
(29), (25), (22), (20), (16), (15), (11), (10), (10), (8), (8), (8), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Souris
(289), (164), (101), (9), (8)
Hôte Souris
(237), (64), (10), (3), (2), (1)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp TEK est conjugé à/à la Alexa Fluor 647
(21), (14), (10), (8), (8), (8), (8), (8), (8), (7), (6), (4), (4), (4), (4), (4), (4), (1)
Application Intracellular Staining (ICS)
(174), (128), (69), (53), (48), (22), (21), (19), (16), (12), (8), (6), (6), (3)
Pubmed 6 références disponible
Fournisseur

Détail du produit anti-TEK anticorps

Détail du antigène TEK Information d'application Stockage References for anti-TEK antibody (ABIN1177217) Images
Marque BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogène Phosphorylated Human TIE2 Peptide
Clone K91-860
Isotype IgG1, kappa
Plasmids, Primers & others

Détail du antigène TEK

Détail du produit anti-TEK anticorps Information d'application Stockage References for anti-TEK antibody (ABIN1177217) Images Haut de la page
Antigène
Autre désignation TIE2 (TEK Antibody Extrait)
Sujet TIE2 (Tyrosine kinase with Immunoglobulin-like and EGF-like domains 2), also known as TEK (Tunical Endothelial Kinase), is an endothelial and hematopoietic cell-specific receptor tyrosine kinase (RTK) that is critical to the development and maintenance of the vasculature and highly conserved among vertebrate species. The angiopoietins are ligands of TIE2, and the abnormal vascular growth that often occurs in solid tumors is a result of disruptions in the coordinated actions of the angiopoietins, TIE2, and the closely related TIE1 RTK. Upon activation by angiopoietins, TIE2 autophosphorylates at least 2 tyrosines in its protein kinase domain and at least 3 tyrosines in its C-terminal tail. The K91-860 monoclonal antibody recognizes the phosphorylated tyrosine 992 (Y992) in the protein kinase domain of activated TIE2. The orthologous phosphorylation site in mouse TIE2 is Y990.
Synonyms: TEK, VMCM, CD202b
Pathways Signalisation RTK, Growth Factor Binding

Information d'application

Détail du produit anti-TEK anticorps Détail du antigène TEK Stockage References for anti-TEK antibody (ABIN1177217) Images Haut de la page
Indications d'application Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.
Volume d'échantillon 20 μL
Restrictions For Research Use only

Stockage

Détail du produit anti-TEK anticorps Détail du antigène TEK Information d'application References for anti-TEK antibody (ABIN1177217) Images Haut de la page
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Stockage commentaire The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TEK antibody (ABIN1177217)

Détail du produit anti-TEK anticorps Détail du antigène TEK Information d'application Stockage Images Haut de la page
Produit citée dans:

Audero, Cascone, Maniero, Napione, Arese, Lanfrancone, Bussolino: "Adaptor ShcA protein binds tyrosine kinase Tie2 receptor and regulates migration and sprouting but not survival of endothelial cells." dans: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 13224-33, 2004

Peters, Kontos, Lin, Wong, Rao, Huang, Dewhirst, Sankar: "Functional significance of Tie2 signaling in the adult vasculature." dans: Recent progress in hormone research, Vol. 59, pp. 51-71, 2004

DeBusk, Hallahan, Lin: "Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway." dans: Experimental cell research, Vol. 298, Issue 1, pp. 167-77, 2004

Jones, Iljin, Dumont, Alitalo: "Tie receptors: new modulators of angiogenic and lymphangiogenic responses." dans: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 257-67, 2001

Murray, Padrique, Pinko, McTigue: "Mechanistic effects of autophosphorylation on receptor tyrosine kinase catalysis: enzymatic characterization of Tie2 and phospho-Tie2." dans: Biochemistry, Vol. 40, Issue 34, pp. 10243-53, 2001

Edgell, McDonald, Graham: "Permanent cell line expressing human factor VIII-related antigen established by hybridization." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 12, pp. 3734-7, 1983