anti-Souris CAMK2A anticorps pour Flow Cytometry

Recommended CAMK2A Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Anticorps
  • camk2
  • camk2b
  • zgc:112538
  • zgc:123320
  • CAMKA
  • CaMKII
  • R74975
  • mKIAA0968
  • PK2CDD
  • PKCCD
  • calcium/calmodulin dependent protein kinase II alpha
  • calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog
  • calcium/calmodulin-dependent protein kinase II alpha
  • CAMK2A
  • camk2a.S
  • camk2a
  • Camk2a
Épitope
Internal Region
27
25
13
11
10
9
6
6
5
5
4
3
2
2
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
144
91
90
26
8
7
5
4
3
3
2
1
1
1
1
1
1
1
1
Hôte
Chèvre
117
58
19
Clonalité
Polyclonal
Conjugué
Cet anticorp CAMK2A est non-conjugé
7
7
7
6
6
6
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
191
99
72
56
46
36
27
10
7
6
4
2
2
1
Options
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N° du produit ABIN570984
$ 429.84
Plus shipping costs $45.00

General

Antigène Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Anticorps
Épitope Internal Region
(27), (25), (13), (11), (10), (9), (6), (6), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Souris, Rat (Rattus)
(144), (91), (90), (26), (8), (7), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Hôte Chèvre
(117), (58), (19)
Clonalité
Polyclonal
Conjugué Cet anticorp CAMK2A est non-conjugé
(7), (7), (7), (6), (6), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(191), (99), (72), (56), (46), (36), (27), (10), (7), (6), (4), (2), (2), (1)
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Détail du produit anti-CAMK2A anticorps

Détail du antigène CAMK2A Information d'application Stockage Images
Fonction CAMK2A
Blocking Peptide Blocking peptide for this product available: ABIN2815316
Séquence PRTAQSEETR VWHR
Specificité This antibody is expected to recognize both reported isoforms (NP_057065.2, NP_741960.1).
Homologie Human, Mouse, Rat
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Classe de qualité Verified
Immunogène Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2, NP_741960.1.
Plasmids, Primers & others

Détail du antigène CAMK2A

Détail du produit anti-CAMK2A anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation CAMK2A (CAMK2A Antibody Extrait)
Sujet CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase IIA, calcium/calmodulin-dependent protein kinase type II alpha chain
ID gène 815
NCBI Accession NP_057065, NP_741960
Pathways Signalisation WNT, Interferon-gamma Pathway, Myometrial Relaxation and Contraction

Information d'application

Détail du produit anti-CAMK2A anticorps Détail du antigène CAMK2A Stockage Images Haut de la page
Indications d'application

DS WB Results: Approx 58-60 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 55.3 kDa according to Human NP_057065.2, and 54.1 kDa according to to Mouse NP_803126.1 and Rat NP_037052.1). An additional band of 50 kDa was observed in some Rat Brain lysates and wss successfully blocked by incubation with the immunizing peptide. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03--0.3 μg/mL. Primary incubation 1 hour at room temperature.

DS IHC Results: In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration: 3-6 μg/mL. Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires

Immunofluorescence: Strong expression of the protein seen in the membranes of U2OS and U251 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only

Stockage

Détail du produit anti-CAMK2A anticorps Détail du antigène CAMK2A Information d'application Images Haut de la page
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Minimize freezing and thawing.
Stock -20 °C
Stockage commentaire Aliquot and store at -20 °C.

Images

Détail du produit anti-CAMK2A anticorps Détail du antigène CAMK2A Information d'application Stockage Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (Internal Region) antibody (ABIN570984) Antibody (0.3µg/ml) staining of Human Cerebellum (A), Mouse (B) and Rat Brain (C) lys...