anti-Singe WNT10A anticorps pour Immunohistochemistry

Recommended WNT10A Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A) Anticorps
  • WNT10A
  • wnt10a/12
  • wnt10a
  • OODD
  • SSPS
  • STHAG4
  • WNT-10A
  • Wnt family member 10A
  • protein Wnt-10a
  • Wnt10a protein
  • hypothetical protein
  • WNT10A protein
  • Wnt family member 10A L homeolog
  • wingless-type MMTV integration site family, member 10A
  • wingless-type MMTV integration site family, member 10a
  • WNT10A
  • LOC778792
  • CpipJ_CPIJ005839
  • Wnt10a
  • wnt10a
  • wnt10a.L
Épitope
Internal Region
11
6
6
4
2
2
2
1
1
Reactivité
Boeuf (Vache), Chien, Hamster, Humain, Singe, Souris, Lapin, Rat (Rattus)
52
22
10
6
6
5
4
4
2
2
1
1
1
Hôte
Lapin
58
1
Clonalité
Polyclonal
Conjugué
Cet anticorp WNT10A est non-conjugé
5
3
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
37
29
20
15
6
6
5
3
1
1
1
Fournisseur
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N° du produit (Fournisseur)
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N° du produit ABIN1049467
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
8.538242 ABIN1049468 IHC IHC (p) Rabbit Internal Region Connectez-vous pour afficher Polyclonal 0
8.538242 ABIN213598 IHC IHC (p) Rabbit Internal Region Connectez-vous pour afficher Polyclonal 0
8.538242 ABIN2469512 IHC Rabbit Internal Region Connectez-vous pour afficher Polyclonal 0

General

Antigène Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A) Anticorps
Épitope Internal Region
(11), (6), (6), (4), (2), (2), (2), (1), (1)
Reactivité Boeuf (Vache), Chien, Hamster, Humain, Singe, Souris, Lapin, Rat (Rattus)
(52), (22), (10), (6), (6), (5), (4), (4), (2), (2), (1), (1), (1)
Hôte Lapin
(58), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp WNT10A est non-conjugé
(5), (3), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(37), (29), (20), (15), (6), (6), (5), (3), (1), (1), (1)
Fournisseur Connectez-vous pour afficher

Détail du produit anti-WNT10A anticorps

Détail du antigène WNT10A Information d'application Stockage Images
Marque IHC-plus™
Specificité Human WNT10A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Rabbit (100%) Elephant, Bat (94%) Chicken (89%) Opossum, Turkey, Lizard, Xenopus, Stickleback, Pufferfish (83%).
Purification Immunoaffinity purified
Immunogène Synthetic 18 amino acid peptide from internal region of human WNT10A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Rabbit (100%), Elephant, Bat (94%), Chicken (89%), Opossum, Turkey, Lizard, Xenopus, Stickleback, Pufferfish (83%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Détail du antigène WNT10A

Détail du produit anti-WNT10A anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation WNT10A (WNT10A Antibody Extrait)
Sujet Name/Gene ID: WNT10A
Family: WNT

Synonyms: WNT10A, SSPS, STHAG4, OODD, Protein Wnt-10a
ID gène 80326
Pathways Signalisation WNT

Information d'application

Détail du produit anti-WNT10A anticorps Détail du antigène WNT10A Stockage Images Haut de la page
Indications d'application Approved: IHC, IHC-P (5 μg/mL)
Commentaires

Target Species of Antibody: Human

Procédure de l'essai The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Stockage

Détail du produit anti-WNT10A anticorps Détail du antigène WNT10A Information d'application Images Haut de la page
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C,-20 °C
Stockage commentaire Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Date de péremption 12 months

Images

Détail du produit anti-WNT10A anticorps Détail du antigène WNT10A Information d'application Stockage Haut de la page
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A) (Internal Region) antibody (ABIN1049467) Anti-WNT10A antibody ABIN1049467 IHC staining of human pancreatic islet. Immunohisto...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A) (Internal Region) antibody (ABIN1049467) Anti-WNT10A antibody IHC of human pancreatic islet. Immunohistochemistry of formalin-...