anti-Boeuf (Vache) IL21 anticorps pour Enzyme Immunoassay

Recommended IL21 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Interleukin 21 (IL21) Anticorps
  • IL-21
  • Za11
  • interleukin 21
  • IL21
  • Il21
Épitope
AA 24-152
23
18
15
13
8
7
6
6
6
5
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivité
Boeuf (Vache)
203
103
24
9
5
5
4
1
1
Hôte
Lapin
164
70
41
11
5
Clonalité
Polyclonal
Conjugué
Cet anticorp IL21 est non-conjugé
20
15
13
11
10
9
6
6
5
5
5
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
185
132
49
39
34
25
15
13
7
6
5
4
4
3
3
2
2
1
1
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N° du produit ABIN401062
$ 533.50
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General

Antigène Interleukin 21 (IL21) Anticorps
Épitope AA 24-152
(23), (18), (15), (13), (8), (7), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivité Boeuf (Vache)
(203), (103), (24), (9), (5), (5), (4), (1), (1)
Hôte Lapin
(164), (70), (41), (11), (5)
Clonalité
Polyclonal
Conjugué Cet anticorp IL21 est non-conjugé
(20), (15), (13), (11), (10), (9), (6), (6), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(185), (132), (49), (39), (34), (25), (15), (13), (7), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1)
Pubmed 1 référence disponible
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Détail du produit anti-IL21 anticorps

Détail du antigène IL21 Information d'application Stockage References for anti-IL21 antibody (ABIN401062) Images
Specificité This antibody reacts to Interleukin-21.
Réactivité croisée (Details) Species reactivity (expected):Porcine, Horse, Human, Canine, Macaque, Platypus and Syrian hamster, Mouse, Rat, Chicken.
Species reactivity (tested):Bovine.
Purification Affinity chromatography on Protein A
Immunogène Recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein
Isotype IgG

Détail du antigène IL21

Détail du produit anti-IL21 anticorps Information d'application Stockage References for anti-IL21 antibody (ABIN401062) Images Haut de la page
Antigène
Autre désignation Interleukin-21 / IL21 (IL21 Antibody Extrait)
Sujet Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common ?-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Ra chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells' (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells, with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81 % and 58-67 % homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MATSynonyms: IL-21, Za11
ID gène 378475
NCBI Accession NP_942129
UniProt Q76LU5
Pathways Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Information d'application

Détail du produit anti-IL21 anticorps Détail du antigène IL21 Stockage References for anti-IL21 antibody (ABIN401062) Images Haut de la page
Indications d'application ELISA: 1/6,000. Western Blot: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Stockage

Détail du produit anti-IL21 anticorps Détail du antigène IL21 Information d'application References for anti-IL21 antibody (ABIN401062) Images Haut de la page
Format Liquid
Concentration 1.0 mg/mL by UV absorbance at 280 nm
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock 4 °C/-20 °C
Stockage commentaire Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-IL21 antibody (ABIN401062)

Détail du produit anti-IL21 anticorps Détail du antigène IL21 Information d'application Stockage Images Haut de la page
Background publications

Muneta, Kikuma, Yoshihara, Mori: "Cloning, expression, and tissue distribution of bovine interleukin-21." dans: Veterinary immunology and immunopathology, Vol. 95, Issue 1-2, pp. 73-80, 2003

Images

Détail du produit anti-IL21 anticorps Détail du antigène IL21 Information d'application Stockage References for anti-IL21 antibody (ABIN401062) Haut de la page
Supplier Images
 image for anti-Interleukin 21 (IL21) (AA 24-152) antibody (ABIN401062) anti-Interleukin 21 (IL21) (AA 24-152) antibody