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Human OCT4 Protein expressed in HEK-293 Cells - ABIN2727872
Soufi, Garcia, Jaroszewicz, Osman, Pellegrini, Zaret: Pioneer transcription factors target partial DNA motifs on nucleosomes to initiate reprogramming. dans Cell 2015
a fundamental role of OCT4A protein in regulating FOS/AP-1 signaling-centered genes that mediate the adhesion, migration, and propagation of somatic cancer cells, is reported.
Results demonstrate that Oct3 governs beta-AR activity as a NE recycling transporter in white adipocytes.
Report differential expression of OCT4 in normal human spermatogenesis, testicular mixed germ cell-sex cord stromal tumor, spermatocytic tumor, and seminoma.
Targeting dCas9 to an Oct4 site in the Nanog promoter displaced Oct4 from this site, reduced Nanog expression, and slowed division. In contrast, disrupting the Oct4 binding site adjacent to Pax6 upregulated Pax6 transcription and disrupting Nanog binding its own promoter upregulated its transcription
Using genetic-lineage-tracing experiments and an in situ labeling approach, we show that DDR-induced epigenetic reactivation of OCT4 regulates the resistance to chemotherapy and contributes to tumor relapse both in mouse and primary human cancers. In turn, deletion of OCT4 reverses chemoresistance and delays the relapse.
Authors conclude that OCT4-PG1 pseudogene plays a key role in the regulation OCT-4 transcription factor, which alters MDR phenotype in the FEPS cell line.
These findings indicate the involvement of the transient patterned methylation of the Oct4A enhancers and alternative splicing in the adaptive regulation of cell fate choice.
PHF20 interacts with poly(ADP-ribose) polymerase 1 (PARP1) and directly binds to promoter regions of octamer-binding transcription factor 4 (OCT4) and sex determining region Y-box 2 (SOX2) to modulate a histone mark associated with active transcription, trimethylation of lysine 4 on histone H3 protein subunit (H3K4me3).
OCT4 is dependent on ERalpha to suppress the proliferation of breast cancer cells through DNMT1/ISL1/ERK axis.
This study assessed the correlation between SOX2 mRNA expression and OCT4 mRNA expression, as well as the association between the clinicopathological indices and both gene expression levels. The relative mRNA expression levels of OCT4 genes in primary pterygium were significantly reduced compared to the normal conjunctiva tissues.
These findings indicate that OCT4 expression is sufficient to sustain intrinsic signaling in a LIF-independent manner to promote ES cell pluripotency and self-renewal.
Genetic and Epigenetic Profiling Reveals EZH2-mediated Down Regulation of OCT-4 Involves NR2F2 during Cardiac Differentiation of Human Embryonic Stem Cells.
MYC and OCT4 were able to bind to the promoter region of miR-9 to trigger its transcription.
Inheritance of OCT4 predetermines fate choice in human embryonic stem cells.
POU5F1B was upregulated in cervical cancer and down-regulation inhibited cell proliferation and migration and induced apoptosis in cervical cancer cell lines by modulating OCT4.
OCT4B(19kDa) may play a crucial role in regulating cancer cell survival and adaption in a rigid environment.
NKX3-1 substitutes for exogenous OCT4 to reprogram both mouse and human fibroblasts at comparable efficiencies and generate fully pluripotent stem cells.
These results suggest that the Oct4 gene may regulate Neuroblastic tumours (NBT) pathogenic differentiation pathways, and should thus be considered as a target for knockdown when developing novel therapies for high-risk NBT patients.
The PAX5-OCT4-PRDM1 proteins form a core transcriptional network that activates germline and represses somatic programmes during human germ cell differentiation.
Nitric oxide (NO) promotes the cancer stem cells -regulatory activity of Oct4 through a mechanism that involves complex formation between Oct4 and the scaffolding protein caveolin-1 (Cav-1). In the absence of NO, Oct4 forms a molecular complex with Cav-1, which promotes the ubiquitin-mediated proteasomal degradation of Oct4.
Cytoplasmic CD44 and absence of nuclear Oct3/4 suggest that the cells of cardiac sarcomas may represent 'daughter' stem cells that no longer have the capacity for tumour initiation, but have subsequently developed new lines of partial differentiation.
Lack of restricted OCT4 protein, and inner cell mass localization of NANOG in primate blastocysts, suggests that NANOG may determine inner cell mass fate more specifically during primate development.
Pou5f3 remodels chromatin on high nucleosome affinity regions at zygotic genome activation.
Western blot analysis were conducted on developing hatchlings with Oct4 antibody and at 13.6 and 21.6mugL(-1)dose,it showed over expression elucidating stimulatory role of nanosilver. In silico analysis depicted a firm interaction of nanosilver with Oct4 revealing their key role in growth stimulation of developing embryos.
The authors show, at single-cell resolution in vivo, that Pou5f3 complexes with Nanog to pattern mesendoderm differentiation at the blastula stage.
Data suggest that, in developing gastrula, Znfl1 controls developmental gene expression of Hoxb1b in embryonic posterior neuroectoderm by acting upstream of Pou5f3 and Sall4; these proteins appear to be involved in neurogenesis. (Znfl1 = zinc finger-like gene 1; Hoxb1b = homeobox B1b protein; Pou5f3 = POU domain class 5 transcription factor 3; Sall4 = spalt-like transcription factor 4)
Regulation of mych by Pou5f1 appears to be direct transcriptional activation.
The posttranslational modification by phosphorylation opens the possibility that Pou5f1 may be subject to temporal or region specific modulation of its activity or stability by embryonic signaling mechanisms.
discuss mechanistic implications of simultaneous activation of transcriptional targets by ubiquitous, like Pou5f1, and region-specific inducers, emerging as a common regulatory motif in early development
maternal Nanog, Pou5f1 and SoxB1 are required to initiate the zygotic developmental program and induce clearance of the maternal program by activating miR-430 expression
thses data position Pou5f1 and SOX-POU sites at the center of the zygotic gene activation network of vertebrates and provide a link between zygotic gene activation and pluripotency control.
The defects due to HEP induction were rescued by introducing wild-type pou2 mRNA before the heat treatments.
Vox plays a key role downstream of BMP signals in regulating the capacity of Nodal to induce endoderm versus mesoderm by modulating the activity of the Casanova/Pou2 regulatory system.
Pou2 functions in multiple aspects of vertebrate development, especially in the binary decision of the mesendoderm to mesoderm and endoderm in different ways depending on the developmental stage.
show that Pou5f1 binds to phylogenetically conserved Oct/Pou5f1 sites in the vox promoter, both in vivo and in vitro
The temporospatial structure of the zebrafish Pou5f1 target networks may explain aspects of the evolution of the mammalian stem cell networks.
Pou2 is essential for endoderm formation. Embryos devoid of maternal and zygotic pou2 lack endodermal precursors.
Data show that embryos genetically depleted of both maternal and zygotic pou2 function exhibit extreme dorsoventral patterning defects and, independently, a blastoderm-specific arrest of epiboly.
Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defects of Pou5f1/Oct4 deficient MZspg zebrafish embryos.
These data provide a scheme wherein pou2/pou5f1 expression in zebrafish embryos is regulated by both an autoregulatory loop and repression by RA emanating from the posterior mesoderm.
ubiquitous expression of the protein in early (Day 4) in vivo-developed rabbit blastocysts and its restriction to the embryonic disk by Day 6
sequences of mRNA and translated protein of the newly identified genes and those of POU5F1 were aligned to their mammalian orthologs to determine the degree of evolutionary conservation
Higher values of OCT-4 expression were observed in embryos and endometrial tissue in females reproduced under heat conditions.
protein expression during rabbit embryonic development: investigation of temporal and spatial relationship of expression of Oct-4, Cdx-2 (caudal type homeobox transcription factor 2) and histone H4 (acetylated at lysine 5) in morula/blastocysts
The signal may have reflected the regulation of Oct-4 through enhancer switching and therefore may be related to cell lineage formation in rabbit embryos.
Cloning of POUF5F1 cDNA and its genomic organization, chromosome localization and expression in adult tissues and embryonic stem cells has been reported.
The POU5F1 gene is strictly regulated during early embryo development.
OCT4 directly activates expression of Ash2l-b.
Cdk1 is an upstream regulator of the Oct4 phosphorylation state during cell cycle progression, and it coordinates the chromatin associated state of Oct4 for pluripotency-related gene expression within the cell cycle.
Reprogramming factors (Oct4, Sox2, Klf4, c-myc) induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes.
Sod1 mRNA levels were reduced when Oct4, Sox2 and Nanog were down-regulated by a shRNA approach in mESCs.
These findings provide new insights into reconsidering Oct4 variants expression and its additional role in maintaining the pluripotency of stem cells.
To determine the reprogramming potential of germ cell- specific genes, a polycistronic gene cassette expressing Stella, Oct4 and Nanos2 in a lentiviral-based vector, was designed.
Knockout of Oct4 in perivascular cells significantly impairs perivascular cell migration, increases perivascular cell death, delays endothelial cell migration, and promotes vascular leakage following corneal angiogenic stimulus. Knockout of Oct4 in perivascular cells also impairs perfusion recovery and decreases angiogenesis following hindlimb ischemia.
The effect of p53 on the capacity of cardiogenic transdifferentiation is evaluated using p53 wild-type (p53(+/+)), p53 heterozygous mutant (p53(+/-)), and p53 homozygous mutant (p53(-/-)) embryonic fibroblasts (MEFs). The effect of an important reprogramming factor Oct4 on cardiac transdifferentiation was also evaluated in the allelic series of p53 MEFs.
Correlation analysis revealed that OCT4B expression was significantly associated with the SLUG level in lung tumors. These results provide novel insights into OCT4B-mediated oncogenesis in cancer dissemination.
we demonstrated that hypoxia significantly induced the GSC mesenchymal transition, increased the expression levels of the pluripotent transcription factor OCT4 and migration-associated proteins..he underlying mechanism involved significant IGF-1/IGF-1R activation of OCT4/CXCR4 expression through HIF-2alpha regulation.
Oct4 is involved in DNA methylation through the regulation of Dnmt1 transcription, especially during the early stages of mouse pre-implantation embryo development.
Zfp127 is a novel regulator of Oct4, and may become a potent target to improve cellular reprogramming.
Here we describe an optimized DamID method coupled with next-generation sequencing (DamID-seq) in mouse cells and demonstrate the identification of the binding sites of two TFs, POU5F1 (also known as OCT4) and SOX2, in as few as 1000 embryonic stem cells (ESCs) and neural stem cells (NSCs), respectively.
Data show that OCT4 protein impedes mouse embryonic stem cells (mESCs) differentiation despite MITF transcription factor (MITF) expression.
These results indicate a role for Oct4 in orchestrating multiple fates and enabling expansion, correct patterning and lineage choice in the postimplantation epiblast.
Acidic pH induced OCT-4 expression in fibroblasts and stromal cells in tumor microenvironment.
Activin A and overexpression of SMAD2/3 significantly promoted expressions of porcine NANOG and OCT4,maintaining induced pluripotent stem cell self-renewal through up-regulation of Nanog/OCT4 expression.
Our results indicate that continuous expression of OCT-4 in blastomeres is essential for trophectoderm formation of porcine embryos.
These findings suggest that the 12-bp indel polymorphism of the Oct4 gene might be a potential DNA marker for selecting preferred individuals in relation to reproductive traits in pig marker-assisted selection breeding.
Oct4-overexpression enhances porcine ovarian stem cell differentiation in to oocyte-like cells.
showed novel molecular regulation of CDX2 on Oct4, and provided important clues for clarifying the mechanism of interaction between CDX2 and Oct4 in embryo of mammals other than mouse
Overexpression of Sox2 or Oct4 in bone mesenchymal stem cells in culture media containing a basic fibroblast growth factor results in higher proliferation and differentiation compared to controls.
Oct4 positive stem/progenitor swine lung epithelial cells are targets for influenza virus replication.
study shows that localization of octamer-binding protein 4(OCT4) is associated with an embryonic stem cell (ESC)-like morphology from porcine inner cell mass
OCT4 expression, in contrast to earlier speculations, at least in hatched blastocysts, resembles the expression pattern in the mouse embryo.
cells isolated from umbilical cord express three transcription factors,Oct-4, Sox-2 & Nanog, found in pluripotent stem cell markers both at the mRNA and protein level.
in the forming endoderm at the primitive streak stage, OCT4 was detectable in potential primordial germ cells only; in the ectoderm and mesodermal cell lineages OCT4 was cleared at the neural groove and somite stage, respectively
CDX2 expression was aberrant in embryos lacking POU5F1
In OCT4 KO morulae (day 5), approximately 70% of the nuclei were OCT4 positive, indicating that maternal OCT4 mRNA partially maintains OCT4 protein expression during early development.
The use of a clonal cell populations with low senescence level, normal karyotype, and highest POU5F1 expression levels did not improve embryo development rates or quality following SCNT. As previously suggested, this study supports the notion that levels of POU5F1 expression in the donor nucleus do not impact the Somatic cell nuclear transfer results.
we concluded that OCT4 expression in somatic cells is not a good prognosis marker for selecting cell lines.
analysis of pluripotency gene expression of OCT4, SOX2 and NANOG and mRNA levels of some of their downstream targets in bovine oocytes and early embryos
Oct4 exhibited significant hypermethylation in sperm compared with that in oocytes
In contrast to protein distribution, regulation of Oct4 transcription is conserved between mammalian species.
analysis of CpG islands of bovine Leptin and POU5F1 genes in cloned bovine fetuses
The restoration of pluripotency can be directly observed in living cells or SCNT embryos from such Pou5f1-EGFP transgenic fetuses.
The expression of glycolysis-related genes about PGAM1, KPYM2 and HXK2 in CiPSC-like colonies formation groups was significantly higher than their parental fibroblasts, but not in the non-CiPSC-like colonies formation group.
Oct4 cDNA and a 2.8-kb regulatory region upstream of its start codon were isolated and characterized
evaluated pattern of POU5F1 expression during early embryo development;study shows POU5F1 protein is present in cytoplasm and nucleus of immature oocytes, decreases during embryo development to day 5, then increases again within embryonic nuclei [POU5F1]
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6\;22)(p21\;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
POU domain transcription factor OCT4
, POU domain, class 5, transcription factor 1
, POU-type homeodomain-containing DNA-binding protein
, octamer-binding protein 3
, octamer-binding protein 4
, octamer-binding transcription factor 3
, octamer-binding transcription factor-3
, POU class 5 homeobox 1
, POU domain class 5 transcription factor 1
, POU domain gene 2
, spiel ohne grenzen
, spiel ohne grenzen/pou2
, octamer binding transcription factor 4
, Octamer-binding transcription factor 3
, octamer-binding transcription factor 4