anti-Humain Complement Factor H anticorps pour Western Blotting

Recommended Complement Factor H Antibody

Antigène
Complement Factor H (CFH) Anticorps
  • AHUS1
  • AMBP1
  • ARMD4
  • ARMS1
  • CFHL3
  • FH
  • FHL1
  • HF
  • HF1
  • HF2
  • HUS
  • Mud-1
  • NOM
  • Sas-1
  • Sas1
  • Fh
  • CFH
  • im:7147412
  • si:ch211-207o17.2
  • fH
  • armd4
  • arms1
  • cfh
  • cfhl3
  • fhl1
  • hf1
  • hf2
  • hus
  • complement factor H
  • complement component factor h
  • complement factor H-related protein 5
  • complement factor H L homeolog
  • CFH
  • Cfh
  • cfh
  • LOC100399888
  • LOC100733902
  • cfh.L
Reactivité
Souris, Rat (Rattus), Humain
234
36
35
3
3
3
3
2
2
Hôte
Lapin
126
87
32
16
1
1
Clonalité
Polyclonal
Conjugué
Cet anticorp Complement Factor H est non-conjugé
22
14
5
4
4
4
4
4
4
4
4
3
2
2
2
2
2
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
172
120
76
42
33
26
24
22
15
10
10
9
9
5
4
1
1
1
1
Options
Testé avec succès

Images

N° du produit ABIN3023097
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
23.534718 ABIN2854706 IHC (p) WB Rabbit IgG Internal Region Polyclonal 0
19.877361 ABIN1173336 ICC IHC WB Rabbit IgG AA 860-1231 Polyclonal 0
19.877361 ABIN2896777 IF/ICC IHC IP WB Rabbit AA 860-1231 Polyclonal 0
17.534718 ABIN185437 ELISA IHC WB Goat Internal Region Polyclonal 1
13.657358 ABIN5621920 WB Rabbit IgG Polyclonal 0
13.657358 ABIN2688490 ELISA WB Mouse IgG1 kappa M81626 0
13.657358 ABIN2688580 ELISA WB Mouse IgG1 kappa M81633 0
13.657358 ABIN2688489 ELISA WB Mouse IgG1 kappa M81609 0
13.657358 ABIN2138054 ELISA WB Mouse IgG1 kappa 34F12 0
13.657358 ABIN285281 ID ELISA WB Goat Polyclonal 0
13.657358 ABIN2137491 ELISA WB Rabbit IgG Polyclonal 0
13.657358 ABIN285280 ID ODD WB Sheep Polyclonal 0
13.657358 ABIN6138452 WB Rabbit Polyclonal 0
13.657358 ABIN6133652 IF IHC WB Rabbit Polyclonal 0
10.87736 ABIN951646 EIA IF IHC (p) WB Rabbit Ig Fraction AA 751-780, Middle Region Polyclonal 3
10.87736 ABIN6260777 ELISA IHC WB Rabbit IgG Polyclonal 0
10.87736 ABIN1871804 IHC WB Rabbit IgG Polyclonal 0
10.87736 ABIN2995930 IF/ICC IHC WB Rabbit IgG Polyclonal 0
10 ABIN462334 ELISA IHC IHC (p) IP WB Mouse IgG1 OX-24 0
8.5 ABIN532661 ELISA WB Mouse IgG1 28A3 3

General

Antigène Complement Factor H (CFH) Anticorps
Reactivité Souris, Rat (Rattus), Humain
(234), (36), (35), (3), (3), (3), (3), (2), (2)
Hôte Lapin
(126), (87), (32), (16), (1), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp Complement Factor H est non-conjugé
(22), (14), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(172), (120), (76), (42), (33), (26), (24), (22), (15), (10), (10), (9), (9), (5), (4), (1), (1), (1), (1)
Fournisseur

Détail du produit anti-Complement Factor H anticorps

Détail du antigène Complement Factor H Information d'application Stockage Images
Purification Affinity purification
Immunogène Recombinant protein of human CFH
Isotype IgG
Plasmids, Primers & others

Détail du antigène Complement Factor H

Détail du produit anti-Complement Factor H anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation CFH (CFH Antibody Extrait)
Sujet CFH (Complement factor H) is a member of the Regulator of Complement Activation (RCA) gene cluster and a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this CFH have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. 1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1, 33:D54-8.
Synonyms: ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS, MGC88246
ID gène 3075
UniProt P08603
Pathways Système du Complément, Cellular Response to Molecule of Bacterial Origin

Information d'application

Détail du produit anti-Complement Factor H anticorps Détail du antigène Complement Factor H Stockage Images Haut de la page
Indications d'application Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Stockage

Détail du produit anti-Complement Factor H anticorps Détail du antigène Complement Factor H Information d'application Images Haut de la page
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze / thaw cycles
Stock -20 °C/-80 °C

Images

Détail du produit anti-Complement Factor H anticorps Détail du antigène Complement Factor H Information d'application Stockage Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-Complement Factor H (CFH) antibody (ABIN3023097) Western blot analysis of extracts of HepG2 cell line, using CFH antibody.
'Independent Validation' signe
Antigène Commplement Factor H
Numéro du lot 4390101
Application validée Immunofluorescence
Contrôle positif MRL/lpr lupus mice (14 weeks of age)
Contrôle négative FH knockout MRL/lpr lupus mice
Conclusion Passed. The complement factor H antibody ABIN3023097 works very well on murine kidney samples and shows the expected CFH localization and strong staining.
Anticorps primaire ABIN3023097
Anticorps secondaire goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
Protocole
  • Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
  • Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
  • Sections were cut at 8µm using the cryostat.
  • Dry sections for 30min at RT.
  • Hydrate sections in PBS 2x for 15min at RT.
  • Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
  • Wash sections 2x with PBS for 15min at RT.
  • Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN3023097, 0004390101) diluted 1:100 in PBS ON at 4°C.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
Validation Images
Immunofluorescence validation image for anti-Complement Factor H (CFH) antibody (ABIN3023097) IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice suffi...