anti-Rat (Rattus) Complement Factor H anticorps pour Western Blotting

Recommended Complement Factor H Antibody

Antigène
Complement Factor H (CFH) Anticorps
  • AHUS1
  • AMBP1
  • ARMD4
  • ARMS1
  • CFHL3
  • FH
  • FHL1
  • HF
  • HF1
  • HF2
  • HUS
  • Mud-1
  • NOM
  • Sas-1
  • Sas1
  • Fh
  • CFH
  • im:7147412
  • si:ch211-207o17.2
  • fH
  • armd4
  • arms1
  • cfh
  • cfhl3
  • fhl1
  • hf1
  • hf2
  • hus
  • complement factor H
  • complement component factor h
  • complement factor H-related protein 5
  • complement factor H L homeolog
  • CFH
  • Cfh
  • cfh
  • LOC100399888
  • LOC100733902
  • cfh.L
Reactivité
Souris, Rat (Rattus), Humain
234
36
35
3
3
3
3
2
2
Hôte
Lapin
126
87
32
16
1
1
Clonalité
Polyclonal
Conjugué
Cet anticorp Complement Factor H est non-conjugé
22
14
5
4
4
4
4
4
4
4
4
3
2
2
2
2
2
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
172
120
76
42
33
26
24
22
15
10
10
9
9
5
4
1
1
1
1
Options
Testé avec succès

Images

N° du produit ABIN3023097
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
16.877264 ABIN1858382 ICC IHC WB Rabbit IgG AA 975-1217 Polyclonal 0
16.877264 ABIN2896779 IF/ICC IHC IP WB Rabbit AA 975-1217 Polyclonal 0
10.877264 ABIN6260777 ELISA IHC WB Rabbit IgG Polyclonal 0
7.8772635 ABIN3201472 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG 0
7.8772635 ABIN6002151 IF/ICC IHC IP WB Mouse AA 975-1217 0
7.8772635 ABIN6002155 IF/ICC IHC IP WB Mouse AA 975-1217 0
7.8772635 ABIN6002153 IF/ICC IHC IP WB Mouse AA 975-1217 0
7.8772635 ABIN5662214 WB Mouse AA 975-1217 0
7.8772635 ABIN5662215 WB Mouse AA 975-1217 0
4.8772635 ABIN6673510 IF IHC WB Rabbit IgG Polyclonal 0
1 ABIN113379 ID IHC (fro) WB Sheep IgG Polyclonal 0
1 ABIN1421104 IHC (p) WB HRP Rabbit IgG Polyclonal 0
1 ABIN2619174 WB FITC Rabbit IgG AA 975-1217 Polyclonal 0
1 ABIN2619171 WB Biotin Rabbit IgG AA 975-1217 Polyclonal 0
1 ABIN1403233 IHC (p) WB Biotin Rabbit IgG Polyclonal 0
1 ABIN1387098 IF (p) IHC (p) WB Rabbit IgG Polyclonal 0
1 ABIN2619168 WB Rabbit IgG AA 975-1217 Polyclonal 0
1 ABIN2878086 IHC WB Rabbit Polyclonal 0
1 ABIN5540274 EIA IHC (fro) WB Mouse IgG1 1A2 0
1 ABIN5540273 EIA IHC (fro) WB FITC Mouse IgG1 1A2 0

General

Antigène Complement Factor H (CFH) Anticorps
Reactivité Souris, Rat (Rattus), Humain
(234), (36), (35), (3), (3), (3), (3), (2), (2)
Hôte Lapin
(126), (87), (32), (16), (1), (1)
Clonalité
Polyclonal
Conjugué Cet anticorp Complement Factor H est non-conjugé
(22), (14), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(172), (120), (76), (42), (33), (26), (24), (22), (15), (10), (10), (9), (9), (5), (4), (1), (1), (1), (1)
Fournisseur

Détail du produit anti-Complement Factor H anticorps

Détail du antigène Complement Factor H Information d'application Stockage Images
Purification Affinity purification
Immunogène Recombinant protein of human CFH
Isotype IgG
Plasmids, Primers & others

Détail du antigène Complement Factor H

Détail du produit anti-Complement Factor H anticorps Information d'application Stockage Images Haut de la page
Antigène
Autre désignation CFH (CFH Antibody Extrait)
Sujet CFH (Complement factor H) is a member of the Regulator of Complement Activation (RCA) gene cluster and a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this CFH have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. 1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1, 33:D54-8.
Synonyms: ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS, MGC88246
ID gène 3075
UniProt P08603
Pathways Système du Complément, Cellular Response to Molecule of Bacterial Origin

Information d'application

Détail du produit anti-Complement Factor H anticorps Détail du antigène Complement Factor H Stockage Images Haut de la page
Indications d'application Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Stockage

Détail du produit anti-Complement Factor H anticorps Détail du antigène Complement Factor H Information d'application Images Haut de la page
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze / thaw cycles
Stock -20 °C/-80 °C

Images

Détail du produit anti-Complement Factor H anticorps Détail du antigène Complement Factor H Information d'application Stockage Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-Complement Factor H (CFH) antibody (ABIN3023097) Western blot analysis of extracts of HepG2 cell line, using CFH antibody.
'Independent Validation' signe
Antigène Commplement Factor H
Numéro du lot 4390101
Application validée Immunofluorescence
Contrôle positif MRL/lpr lupus mice (14 weeks of age)
Contrôle négative FH knockout MRL/lpr lupus mice
Conclusion Passed. The complement factor H antibody ABIN3023097 works very well on murine kidney samples and shows the expected CFH localization and strong staining.
Anticorps primaire ABIN3023097
Anticorps secondaire goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
Protocole
  • Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
  • Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
  • Sections were cut at 8µm using the cryostat.
  • Dry sections for 30min at RT.
  • Hydrate sections in PBS 2x for 15min at RT.
  • Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
  • Wash sections 2x with PBS for 15min at RT.
  • Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN3023097, 0004390101) diluted 1:100 in PBS ON at 4°C.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
Validation Images
Immunofluorescence validation image for anti-Complement Factor H (CFH) antibody (ABIN3023097) IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice suffi...