anti-Rat (Rattus) Cytoplasmic Protein NCK1 anticorps pour Immunofluorescence

Recommended Cytoplasmic Protein NCK1 Antibody (fourni par: Connectez-vous pour afficher )

Antigène
NCK Adaptor Protein 1 (NCK1) Anticorps
  • NCK
  • NCKalpha
  • nck-1
  • NCK1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • Nck1
  • nck
  • nck1-a
  • nck1-b
  • nckalpha
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK adaptor protein 1b
  • NCK adaptor protein 1 L homeolog
  • NCK1
  • Nck1
  • nck1b
  • nck1.L
Épitope
AA 279-377
24
23
11
10
7
5
3
3
2
2
1
1
1
1
1
1
1
Reactivité
Poulet, Boeuf (Vache), Chien, Grenouille, Humain, Souris, Rat (Rattus)
146
30
24
19
7
4
4
3
3
2
2
2
2
2
1
1
1
1
Hôte
Souris
76
69
1
Clonalité
Monoclonal
Conjugué
Cet anticorp Cytoplasmic Protein NCK1 est non-conjugé
6
6
6
6
6
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
137
91
65
26
14
14
7
5
4
2
2
1
1
Options
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N° du produit ABIN967744
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
11.93691 ABIN967743 IF IP IHC WB Mouse IgG2b AA 279-377 Connectez-vous pour afficher 108-NCK 5
11.93691 ABIN3197734 IC IF WB Rabbit Connectez-vous pour afficher Polyclonal 0
1 ABIN1873839 IF IHC WB Rabbit IgG Connectez-vous pour afficher Polyclonal 0
1 ABIN2888058 IF IHC WB Rabbit IgG Connectez-vous pour afficher Polyclonal 0
1 ABIN5697483 ELISA FACS IF IHC IP WB Rabbit IgG Connectez-vous pour afficher Polyclonal 0
1 ABIN1089241 IF IHC ELISA WB Rabbit IgG Connectez-vous pour afficher Polyclonal 0

General

Antigène NCK Adaptor Protein 1 (NCK1) Anticorps
Épitope AA 279-377
(24), (23), (11), (10), (7), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivité Poulet, Boeuf (Vache), Chien, Grenouille, Humain, Souris, Rat (Rattus)
(146), (30), (24), (19), (7), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Hôte Souris
(76), (69), (1)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp Cytoplasmic Protein NCK1 est non-conjugé
(6), (6), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(137), (91), (65), (26), (14), (14), (7), (5), (4), (2), (2), (1), (1)
Pubmed 5 références disponible
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Détail du produit anti-Cytoplasmic Protein NCK1 anticorps

Détail du antigène Cytoplasmic Protein NCK1 Information d'application Stockage References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) Images
 Réactivité croisée Boeuf (Vache), Poulet, Chien, Grenouille, Souris, Rat (Rattus)
Attributs du produit 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogène Human NCK aa. 279-377
Clone 108-NCK
Isotype IgG2b

Détail du antigène Cytoplasmic Protein NCK1

Détail du produit anti-Cytoplasmic Protein NCK1 anticorps Information d'application Stockage References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) Images Haut de la page
Antigène
Autre désignation NCK (NCK1 Antibody Extrait)
Sujet NCK is a 47 kDa protein that belongs to a class of signaling molecules called adaptors. These proteins exert their effects by coupling activated receptors to downstream effectors. NCK is comprised almost entirely of one SH2 and three SH3 domains and it possesses no intrinsic catalytic activity. Investigations of its interactions with cellular proteins have found constitutive association of NCK with various Ser/THr kinases, such as p21-associated kinase (PAK) and casein kinase 1. Other proteins that directly interact with NCK are: Sos, Cbl, Wiskott-Aldrich syndrome protein (WASP), IRS1, and multiple tyrosine kinase receptors. The presence of NCK is necessary for the mitogenic response elicited by PDGF in some cells. In addition, the Drosophila homologue of NCK is involved in regulating cytoskeletal organization. This antibody is routinely tested by western blot analysis.
Poids moléculaire 47 kDa
Pathways TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Information d'application

Détail du produit anti-Cytoplasmic Protein NCK1 anticorps Détail du antigène Cytoplasmic Protein NCK1 Stockage References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) Images Haut de la page
Commentaires

Related Products: ABIN967389

Restrictions For Research Use only

Stockage

Détail du produit anti-Cytoplasmic Protein NCK1 anticorps Détail du antigène Cytoplasmic Protein NCK1 Information d'application References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) Images Haut de la page
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Stockage commentaire Store undiluted at -20° C.

References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744)

Détail du produit anti-Cytoplasmic Protein NCK1 anticorps Détail du antigène Cytoplasmic Protein NCK1 Information d'application Stockage Images Haut de la page
Produit citée dans:

Dalva, Takasu, Lin, Shamah, Hu, Gale, Greenberg: "EphB receptors interact with NMDA receptors and regulate excitatory synapse formation." dans: Cell, Vol. 103, Issue 6, pp. 945-56, 2001

Miyoshi-Akiyama, Aleman, Smith, Adler, Mayer: "Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor." dans: Oncogene, Vol. 20, Issue 30, pp. 4058-69, 2001

Schmitz, Thömmes, Beier, Wagner, Sachinidis, Düsing, Vetter: "Angiotensin II-induced stimulation of p21-activated kinase and c-Jun NH2-terminal kinase is mediated by Rac1 and Nck." dans: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22003-10, 2001

McManus, Boerner, Danielsen, Wang, Matsumura, Maihle: "An oncogenic epidermal growth factor receptor signals via a p21-activated kinase-caldesmon-myosin phosphotyrosine complex." dans: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35328-34, 2000

Roche, McGlade, Jones, Gish, Pawson, Courtneidge et al.: "Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent ..." dans: The EMBO journal, Vol. 15, Issue 18, pp. 4940-8, 1996

Images

Détail du produit anti-Cytoplasmic Protein NCK1 anticorps Détail du antigène Cytoplasmic Protein NCK1 Information d'application Stockage References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) Western blot anlaysis of NCK on HeLa lysate. Lane 1: 1:1000, lane 2: 1: 2000, lane 3:...
Immunofluorescence (IF) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) Immunofluorescent staining on HISM cells.
 image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (Image 3)
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (Image 4)