Pyruvate Dehydrogenase E1 alpha (AA 39-295) anticorps

N° du produit ABIN1886427

Cet anticorps anti-Pyruvate Dehydrogenase E1 alpha Polyclonal Lapin (ABIN1886427) détecte spécifiquement Pyruvate Dehydrogenase E1 alpha dans WB et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Pyruvate Dehydrogenase E1 alpha (AA 39-295) anticorps (ABIN1886427)

Antigène: Pyruvate Dehydrogenase E1 alpha

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit

Épitope: AA 39-295

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 39 and 295 of Pyruvate Dehydrogenase E1 alpha

Information d'application

Indications d'application: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: Biohazard Informations: This product contains thimerosal which is hazardous.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détail du antigène

Antigène: Pyruvate Dehydrogenase E1 alpha

Sujet: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA.The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4).The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits.The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

Poids moléculaire: 43 kDa

ID gène: 5160

NCBI Accession: NM_000284, NP_000275