+49 (0)241 95 163 153
+49 (0)241 95 163 155

RFP anticorps

RFP Reactivité: Discosoma WB, ELISA, IF, IP, IHC, FACS, IHC (fro), IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN129578
  • Highlights
    • Fréquemment cité dans des publications scientifiques avec plus de 300 citations
    • Validé en interne pour des applications pertinentes
    • Rapport de validation détaillé de l'IF fourni par l'un de vos pairs
    • Conçu pour détecter le RFP et ses variantes
    Antigène Voir toutes RFP Anticorps
    RFP (Red Fluorescent Protein (RFP))
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    Cet anticorp RFP est non-conjugé
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    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Polyclonal RFP antibody is designed to detect RFP and its variants.
    Réactivité croisée (Details)
    Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma).
    No reaction was observed against Human, Mouse or Rat serum proteins.
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Sterile filtered
    The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
    Immunogen Type: Recombinant Protein
    Informations sur le produit

    À quoi peut servir l'anticorps RFP ABIN129578 ? Cet anticorps polyclonal RFP détecte RFP et ses variantes, telles que mCherry, tdTomato, mBanana, mOrange et autres. L'anticorps RFP a été validé pour diverses applications et peut être utilisé pour la détection de RFP et de ses dérivés par ELISA, immunofluorescence, FACS, immunohistochimie ou western blotting.

    Quelles sont les données de validation disponibles pour cet anticorps RFP ? L'anticorps est référencé dans plusieurs centaines de publications et se caractérise par une fiabilité éprouvée et très élevée. Il dispose actuellement de 12 images de produits qui montrent ses performances dans une variété d'applications. Le produit est actuellement disponible en quantités de 10µl et 100µl. Anticorps RFP pour la détection de la protéine fluorescente rouge et de ses dérivés. Il s'agit de l'anticorps RFP.

    Quelle est la fonction de RFP ? La RFP est un fluorophore qui émet une fluorescence rouge-orange lorsqu'il est excité. Cette protéine a de nombreuses applications, notamment comme marqueur dans le suivi des processus physiologiques in vivo. Le maximum d'excitation est d'environ 555nm, le maximum d'émission est de 584nm. Les anticorps anti-RFP constituent un moyen assez facile à utiliser pour visualiser la RFP dans ses diverses applications.

  • Indications d'application
    Suggested dilutions:
    ELISA: 1:28,700 - 1:48,700
    IF: 1:200 - 1:2,000
    FACS: 1:200 - 1:2,000
    WB: 1:1,000 - 1:5,000
    IHC: 1:200 - 1:2,000
    IP: User Optimized

    This RFP antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen.
    Optimal titers for applications should be determined by the researcher.

    For Research Use only
  • Validation #101103 (Immunofluorescence)
    'Independent Validation' signe
    Centre d'Immunologie de Marseille-Luminy
    Numéro du lot
    Application validée
    Contrôle positif
    CX3CL1mcherry+ murine thymus
    Contrôle négative
    CX3CL1 WT murine thymus

    Passed. The RFP antibody ABIN129578 specifically labels RFP+ cells in CX3CL1mcherry+ murine thymus in immunofluorescence, consistent with the expression pattern of CX3CL1mcherry.

    'Independent Validation' signe
    Validation Images
    Anticorps primaire
    Anticorps secondaire
    donkey Fab'2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806)
    Full Protocol
    • Harvest thymus from 7 weeks old mice in 1X Dulbecco's phosphate buffered saline (DPBS) (Gibco Life Technologies, 14200-067).
    • Fix mouse thymus in antigen fix (Diapath, P0014) for 2h at 4?C.
    • Wash tissue in 0.1M pH7.4 phosphate buffer at for 1h at 4?C.
    • Dehydrate tissue in 30% sucrose solution at 4?C ON.
    • Snap freeze tissue in Tissue Freezing Medium (ElectroMicroscopy Science, 72592-C) at -80?C.
    • Cut blocks into 25?m sections using a cryostat (Leica, CM3050 S).
    • Transfer sections to a slide.
    • Create a hydrophobic barrier on the slide around sections with Dako pen (Dako, S2002, lot 00081640).
    • Place slide in a humidified chamber and rehydrate sections in 0.1 M TrisHCl pH7.4 for 10min at RT.
    • Gently remove buffer by tapping slide.
    • Permeabilize tissue in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, lot 015K0039) and 0.5% BSA, for 20min at RT.
    • Incubate sections with primary RFP antibody (Red Fluorescent Protein) (AA 234) (antibodies-online, ABIN129578, lot 34945) diluted 1:1000 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 2h at RT.
    • Incubate a no primary antibody negative control in parallel in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA.
    • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
    • Incubate sections with secondary donkey Fab'2 anti-rabbit AF647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806) diluted 1:300 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 2h at RT.
    • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
    • Add approximately 10µL of Slowfade Gold antifade reagent (ThermoFisher Scientific, S36937, lot 1226836) for each section and mount cover slip.
    • Image acquisition on an LSM 880 (Zeiss), 20x magnification, 1000 resolution.
  • Format
    1.0 mg/mL
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid cycles of freezing and thawing.
    4 °C/-20 °C
    Stockage commentaire
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Simpson Ragdale, Clements, Tang, Deltcheva, Andreassi, Lai, Chang, Pandrea, Andrew, Game, Uddin, Ellis, Enver, Riccio, Marguerat, Parrinello: "Injury primes mutation-bearing astrocytes for dedifferentiation in later life." dans: Current biology : CB, (2023) (PubMed).

    Amodeo, Davies, Martinez-Segura, Clements, Ragdale, Bailey, Dos Santos, MacRae, Mokochinski, Kramer, Garcia-Diaz, Gould, Marguerat, Parrinello: "Diet suppresses glioblastoma initiation in mice by maintaining quiescence of mutation-bearing neural stem cells." dans: Developmental cell, (2023) (PubMed).

    Scholz, Dahse, Kemkemer, Bormann, Auger, Vieira Contreras, Ernst, Staake, Körner, Buhlan, Meyer-Mölck, Chung, Blanco-Redondo, Klose, Jarboui, Ljaschenko, Bigl, Langenhan: "Molecular sensing of mechano- and ligand-dependent adhesion GPCR dissociation." dans: Nature, Vol. 615, Issue 7954, pp. 945-953, (2023) (PubMed).

    Middelkamp, Ruck, Krisp, Sumisławski, Mohammadi, Dottermusch, Meister, Küster, Schlüter, Windhorst, Neumann: "Overexpression of Lin28A in neural progenitor cells in vivo does not lead to brain tumor formation but results in reduced spine density." dans: Acta neuropathologica communications, Vol. 9, Issue 1, pp. 185, (2022) (PubMed).

    Pouchelon, Vergara, McMahon, Gorissen, Lin, Vormstein-Schneider, Niehaus, Burbridge, Wester, Sherer, Fernandez-Otero, Allaway, Pelkey, Chittajallu, McBain, Fan, Nasse, Wildenberg, Fishell et al.: "A versatile viral toolkit for functional discovery in the nervous system. ..." dans: Cell reports methods, Vol. 2, Issue 6, pp. 100225, (2022) (PubMed).

    Dumoulin, Zuñiga, Stoeckli: "Axon guidance at the spinal cord midline-A live imaging perspective." dans: The Journal of comparative neurology, (2021) (PubMed).

    Park, Lofton, Li, Rasin: "Extrinsic Regulators of mRNA Translation in Developing Brain: Story of WNTs." dans: Cells, Vol. 10, Issue 2, (2021) (PubMed).

    Dottermusch, Sumisławski, Krevet, Middelkamp, Voß, Siebels, Bartsch, Sotlar, Meyer, Frank, Korshunov, Glatzel, Schüller, Neumann: "Co-activation of Sonic hedgehog and Wnt signaling in murine retinal precursor cells drives ocular lesions with features of intraocular medulloepithelioma." dans: Oncogenesis, Vol. 10, Issue 11, pp. 78, (2021) (PubMed).

    Brooks, Clements, Burden, Kocher, Richards, Devesa, Zakka, Woodberry, Ellis, Jaunmuktane, Brandner, Morrison, Pollard, Dirks, Marguerat, Parrinello: "The white matter is a pro-differentiative niche for glioblastoma." dans: Nature communications, Vol. 12, Issue 1, pp. 2184, (2021) (PubMed).

    Dannhäuser, Lux, Hu, Selcho, Chen, Ehmann, Sachidanandan, Stopp, Pauls, Pawlak, Langenhan, Soba, Rittner, Kittel: "Antinociceptive modulation by the adhesion GPCR CIRL promotes mechanosensory signal discrimination." dans: eLife, Vol. 9, (2021) (PubMed).

    Bellomo, Mondor, Spinelli, Lagueyrie, Stewart, Brouilly, Malissen, Clatworthy, Bajénoff: "Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages." dans: Immunity, Vol. 53, Issue 1, pp. 127-142.e7, (2020) (PubMed).

    Eun, Hong, Jeong, Park, Hwang, Jeong, Choi, Olsson, Hwang, Hyun, Kim: "Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer." dans: PLoS ONE, Vol. 15, Issue 6, pp. e0233784, (2020) (PubMed).

    Fu, Wang, Chouairi, Rose, Abetov, Miller, Yamulla, Schimenti, Flesken-Nikitin, Nikitin: "Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells." dans: Nature communications, Vol. 11, Issue 1, pp. 84, (2020) (PubMed).

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    Green, Cavey, Médina Caturegli, Aigouy, Gompel, Prudhomme: "Evolution of Ovipositor Length in Drosophila suzukii Is Driven by Enhanced Cell Size Expansion and Anisotropic Tissue Reorganization." dans: Current biology : CB, Vol. 29, Issue 12, pp. 2075-2082.e6, (2020) (PubMed).

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    Zhou, Zhong, Peng, Liu, Ding, Sun, Ma, Liu, Chen, Wu, Wang: "Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus." dans: Nature communications, Vol. 11, Issue 1, pp. 4063, (2020) (PubMed).

    Klouda, Condon, Hao, Tian, Lvova, Chakraborty, Nicolls, Zhou, Raby, Yuan: "From 2D to 3D: Promising Advances in Imaging Lung Structure." dans: Frontiers in medicine, Vol. 7, pp. 343, (2020) (PubMed).

    Ricci, Tycksen, Celik, Belle, Fontana, Civitelli, Faccio: "Osterix-Cre marks distinct subsets of CD45- and CD45+ stromal populations in extra-skeletal tumors with pro-tumorigenic characteristics." dans: eLife, Vol. 9, (2020) (PubMed).

    Kennedy, Rinker, Broadie: "Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model." dans: BMC biology, Vol. 18, Issue 1, pp. 94, (2020) (PubMed).

  • Antigène
    RFP (Red Fluorescent Protein (RFP))
    Autre désignation
    RFP (RFP Produits)
    Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
    Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure.
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