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Independently validated

Here you can find our antibodies which have been validated through our Independent Validation Initiative(IVI).

The IVI is centered around core values of transparency, reproducibility, scientific integrity. We are committed to improving the antibody and antibody market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data. Our support can answer further questions via phone, live chat or email.

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250 Résultats :

RFP anticorps

RFP Reactivité: Discosoma WB, ELISA, IF, IHC, IP, FACS, IHC (fro), IHC (p) Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1043867)

Western Blot of Rabbit anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunohistochemistry of Anti-RFP Antibody.

Western Blot of Rabbit Anti-RFP MW hu, ms, rt Antibody.

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody.

Western Blot of Rabbit anti-RFP Antibody.

ELISA Results of Polyclonal Rabbit Anti-RFP Antibody tested against purified RFP protein.

Western Blot of RFP Antibody Pre-Absorbed.

12 images

356 références

Independent Validation

1 validation

N° du produit ABIN129578

Fiche technique

Fiche de données

Independently Validated

COL1A2/COL1A1 anticorps

Rockland 600-401-103-0.1 Reactivité: Humain, Boeuf (Vache), Souris, Porc, Rat ELISA, WB, DB, FLISA, FACS, FM, IHC, IP Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane).

Immunofluorescence staining of extracellular and intracellular type I collagen.

Reduced secretion of type I collagen by Akt inhibition and S451A overexpression.

Anti-fibrotic effect of apigenin in human HSC line (TWNT-4 cells).

The testing of multi-differentiation of rabbit TSCs in vivo.

ASH1 positively influences the expression of TIMP-1, TGF-β-1, and Collagen I.

LPA increases α-SMA and ECM production in HTM cells.

AICAR treatment reduces post-MI adverse remodeling.

SMT022357 improves the pathology in the diaphragm.

Histological analysis of muscle and tendon monoculture tissue model differentiation.

CYD0682 suppresses endogenous ECM protein expression.

Collagen type I expression in the IVD tissue.

Both serum-free and serum-containing fibrocytes express both haematopoietic and stromal cell markers.

The augmented participation from infiltrating monocytes into myeloid-dependent fibrosis in the aging B6J heart.

Immunoperoxidase stain for collagen type I (a–c), collagen type III (d–f) and collagen type VI (g–i).

At least two grid per tissue biopsies were prepared.

Collagen Far Western blot of B.

Immunohistochemistry of Collagen I antibody.

Immunohistochemistry of Collagen I antibody Tissue: human liung Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Strong staining was observed in the extracellular matrix of the lung.

Effect of ex vivo treatment with Pirfenidone and Nintedanib on the fibrotic phenotype of 3D-LTCs.

SIM mutant as dominant negative protein for type I collagen expression.

Transgenic VEGFA overexpression reduced bleomycin-induced lung fibrosis.

Zoledronic acid (ZA) at the maximum concentration in serum (Cmax) inhibits the transforming growth factor-β (TGF-β)-induced myofibroblast (MF) differentiation of human gingival fibroblasts (hGFs).

HJC0123 inhibits hepatic stellate cells activation and decreases endogenous ECM proteins expression.

Recombinant ACLP inhibits adipogenesis and enhances myofibroblast differentiation in human adipose stromal cells.

Age-related alterations in collagen expression and structure.

CCR2 mediates the specific accumulation of type I collagen positive exudate macrophages and Ly-6Chigh monocytes in mice with targeted type II AEC injury.

Oridonin suppresses α-SMA and ECM protein expression.

Immunohistochemistry of Rabbit Anti-collagen type I antibody.

CYD0692 suppresses endogenous and TGF-β induced ECM protein expression.

Immunofluorescence and SHG detection of collagens in NAFLD stage 0 fibrosis.

Inhibition of mTORC1 reduces secretion of type I collagen.

p21 knockout alleviates liver fibrosis.

Decreased gene and protein expression of Tnmd in Scx-deficient mice.

Representative histological analysis for collagen quantification of (a) native and (b) decellularized equine lung tissue.

Western blot of Human Collagen Type I.

Expression of collagen by 5 day cultured spleen cells.

Pulmonary fibroblasts deposit elastin and collagen within Gelfoam matrices.

Inhibition of collagen prolyl hydroxylase by PDC significantly reduced collagen production in vitro and attenuated BLM-induced lung fibrosis in vivo.

Immunofluorescence staining of extracellular and intracellular type I collagen.

Immunofluorescence staining of extracellular and intracellular type I collagen.

46 images

62 références

Independent Validation

2 validations

N° du produit ABIN5596819

Fiche technique

Fiche de données

Independently Validated

Dendra 2 anticorps

Reactivité: Corail WB, FACS, ICC Hôte: Lapin Polyclonal unconjugated

Immunocytochemistry validation image for anti-Dendra 2 antibody (ABIN361314)

Localization of Notch::Dendracrispr (anti-Dendra (ABIN361314), green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells (D, n=9) and in strat SOP daughter cells expressing pnrGAL4>AP-47dsRNA (E, n=20).

Western blotting analysis of Dendra2/GPI fusion protein using rabbit polyclonal antibody PAb (836) on lysates of HEK293T/17 cells transfected with Dendra2/GPI construct, reducing and non-reducing conditions.

WB: line1 – Plum protein 27 kDa, line2 – E.

Localization of NiDendra (anti-Dendra, green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells and in strat SOP daughter cells expressing pnr-GAL4>AP-47dsRNA.

Single optical section images showing Dendra2-NTR (green), Tomato (red), Bhmt (blue), and DAPI (gray) expression in regenerating livers of the WT and lvl at R48h with control (top panel) and Mtz treatment (bottom panel).

Flow cytometry surface staining pattern of HEK293T/17 cells transfected with Dendra2/GPI construct stained using anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC).

Separation of HEK293T/17 cells transfected with Dendra2/GPI construct stained anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC, red-filled) from HEK293T/17 cells transfected with Dendra2/GPI construct unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/Dendra2/GPI cell suspension.

8 images

16 références

Independent Validation

1 validation

N° du produit ABIN361314

Fiche technique

Fiche de données

Independently Validated

E-cadherin anticorps (AA 401-500)

CDH1 Reactivité: Humain, Souris, Rat WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847)

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847)

Human MCF-7 lysates probed with Anti-E cadherin Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Mouse splenocytes probed with Rabbit Anti-E cadherin Polyclonal Antibody, PE-Cy5.

MCF-7 cells were stained with E cadherin Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody.

Image provided by One World Lab validation program.

Image provided by Independent Validation badge 29768.

Image provided by One World Lab validation program.

E-cadherin, N-cadherin, and vimentin expression in PGCCs with budding and control MCF-7 and MDA-MB-231 cells.

Expression of E-cadherin, N-cadherin, and vimentin in human breast tumor tissues.

Expression of E-cadherin, N-cadherin, and vimentin in single stroma PGCCs of human breast cancer.

14 images

14 références

Independent Validation

1 validation

N° du produit ABIN1387847

Fiche technique

Fiche de données

Independently Validated

Caspase 8 anticorps (AA 411-482)

CASP8 Reactivité: Humain, Souris, Rat, Singe, Porc, Mouton WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Caspase 8 (CASP8) (AA 411-482) antibody (ABIN724205)

Image provided by the Independent Validation Program (badge number 29759).

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase-8 Polyclonal Antibody, Unconjugated (ABIN724205) 1:200 followed by conjugation to the secondary antibody and DAB staining

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (ABIN724205) at 1:200 overnight at 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

6 images

15 références

Independent Validation

1 validation

N° du produit ABIN724205

Fiche technique

Fiche de données

Independently Validated

TNF alpha anticorps (AA 181-235)

TNF alpha Reactivité: Humain, Souris, Rat, Lapin, Cheval WB, ELISA, IHC (p), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Histology and immunofluorescence in rat corneal sections.

Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Protein expression of PPARγ, IL-6, and TNF-α in WAT after vibration for 8 weeks.

11 images

21 références

Independent Validation

1 validation

N° du produit ABIN677318

Fiche technique

Fiche de données

Independently Validated

CD63 anticorps (AA 120-175)

CD63 Reactivité: Humain, Souris, Rat, Singe, Chien FACS, WB, IHC, IF Hôte: Chèvre Polyclonal unconjugated

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Anti-CD63 Ab at 1/1,000 dilution; Iysate at 50 µg per lane; Rabbit polyclonal to goat lgG (HRP) at 1/10,000 dilution

Immunofluorescence — anti-CD63 Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA;

Immuno-localization of CD63 in the hen reproductive trac

Representative immunogold labeling (using TEM) images of CSF-derived EVs from control and prenatally cocaine-exposed male and female monkeys are shown.

Flow cytometry analysis of membrane CD63 level in PMN of control and SCK cows.

Expression of exosomes in bronchoalveolar lavage in Rat BAL fluid Source: PMID36035371

Expression of exosomes in Rat lung tissue Source: PMID36035371

Expression of exosomes in Rat Brain Tissue Source: PMID36035371

Immunofluorescence (IF) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting (WB) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting (WB) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

18 images

10 références

Independent Validation

3 validations

N° du produit ABIN1440014

Fiche technique

Fiche de données

Independently Validated

SOX2 anticorps

KO Validated SOX2 Reactivité: Humain WB, IF, FACS, ICC, IHC (p), IP, IHC (fro), CUT&RUN Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (ABIN2855074)

Cleavage Under Targets and Release Using Nuclease validation image for anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (ABIN2855074)

WB Image SOX2 antibody detects SOX2 protein by western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2, antibody at 1:100 dilution.

IP Image Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] , or SOX2 antibody [GT1876], Western blot analysis was performed using SOX2 antibody [N1C3], EasyBlot anti-Rabbit IgG was used as a secondary reagent.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2, antibody at 1:160 dilution.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2, antibody at 1:80 dilution.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis.

FACS Image Flow cytometry on human embryonic stem cells, staining with SOX2 ,antibody at 1:100 dilution(purple) or rabbit IgG (black).

WB Image SOX2 antibody detects SOX2 protein by western blot analysis.

Western blot analysis of CD44, octamer-binding transcription factor 4 (OCT4), sex determining region Y-box 2 (SOX2), SOX9, transketolase (TKT), hypoxia-inducible factor 1 alpha (HIF-α) and phosphoglycerate kinase 1 (PGK-1) in CSC-like and non-CSC UM1 cells (A).

KD of Cops2, but not any other CSN subunits, reduces the expression of Nanog and Oct4.

In vitro characterizations of hc-iPSCs.

S1PR1 activation enhanced NSCs proliferation and neuronal differentiation in hippocampal dentate gyrus (DG) after TBI.

S1PR1-induced NSCs proliferation and neuronal differentiation in TBI rats were affected by MEK/Erk activity.

17 images

11 références

Independent Validation

1 validation

N° du produit ABIN2855074

Fiche technique

Fiche de données

Independently Validated

Iba1 anticorps (C-Term)

IBA1 Reactivité: Humain WB, IHC, IF, ICC, IHC (p), FACS, IHC (fro), IHC (ff) Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody (ABIN2857032)

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.

WB Image Iba1 antibody detects Iba1 protein by western blot analysis.

IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody antibody diluted at 1:1000

IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1, antibody at 1:500 dilution.

WB Image Iba1 antibody detects Iba1 protein by Western blot analysis.

ASI administration deactivated microglial activation and reduced inflammatory responses in spinal cords of EAE mice.

Neuropathology in the central nervous system of Fuca1-deficient mice.

W20 attenuated neuropathology and gliosis in the brains of A53T α-synuclein mice.

W20 reduced neuropathology and neuroinflammation in BACHD mice.

Absence of neuroinflammation in RNase H2ΔGFAP mice.

Effects of NADPH oxidase subunit deletion on plasma TNFα levels and microglial activation after LPS injection.

DPI post-treatment attenuated microglia activation in brain after LPS injection.

Expression of microglial marker protein characteristic for the homeostatic or disease-associated stateAImmunoblot analysis of ApoE, CLEC7A, TREM2, and CD68 in lysates of acutely isolated microglia from Grn-/- and Grn+/+ mice (9 months of age, n = 3 per genotype, female).

Development of myeloma with associated spinal glial cell activation.

18 images

13 références

Independent Validation

1 validation

N° du produit ABIN2857032

Fiche technique

Fiche de données

Independently Validated

beta Amyloid anticorps (C-Term)

Abeta Reactivité: Humain, Souris, Rat WB, ELISA, IF Hôte: Souris Monoclonal 9E8-1A3 unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (Abeta) (C-Term) antibody (ABIN5508784)

Immunohistochemistry validation image for anti-Amyloid beta (Abeta) (C-Term) antibody (ABIN5508784)

Western blots of the monoclonal antibody binding to different amyloid beta (Abeta) regions of human and mouse protein, using 12% A/B (acrylamide/bisacrylamide) or 10% Tricine matrix.

Fluorescent microscopy image (appotome) of 6 months-old APP/PS1 mouse hypocampus and somatosensory cortex.

Immunofluorescence slices with the anti-amyloid beta antibody from different mouse genotypes.

Western blots of monoclonal antibody reactivity with brain extracts from 6 to 15 month-old transgenic (tg) APP/PS1 and wild-type (wt) mice.

6 images

4 références

Independent Validation

2 validations

N° du produit ABIN5508784

Fiche technique

Fiche de données

Independently Validated

CD200 anticorps (Internal Region)

CD200 Reactivité: Humain WB, ELISA, IHC (p) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD200 (CD200) (Internal Region) antibody (ABIN3187966)

Western Blotting (WB) image for anti-CD200 (CD200) (Internal Region) antibody (ABIN3187966)

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:100.

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:100.

6 images

1 reference

Independent Validation

1 validation

N° du produit ABIN3187966

Fiche technique

Fiche de données

Independently Validated

CD4 anticorps (AA 385-457)

CD4 Reactivité: Humain, Souris, Rat, Poulet, Porc FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD4 (CD4) (AA 385-457) antibody (ABIN671376)

Immunohistochemistry validation image for anti-CD4 (CD4) (AA 385-457) antibody (ABIN671376)

Immunohistochemistry validation image for anti-CD4 (CD4) (AA 385-457) antibody (ABIN671376)

Immunohistochemistry validation image for anti-CD4 (CD4) (AA 385-457) antibody (ABIN671376)

Mouse spleen cells probed with Rabbit Anti-CD4 Polyclonal Antibody, PE Conjugated for 30 minutes at 1:50.

Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (ABIN671376) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated (ABIN671376) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Image was kindly submitted by Dr.

Independently Validated Antibody, image provided by Science Direct, badge number 029634: Mouse spleen stained with anti-CD4 (brown) and counterstained with hematoxylin at 1:400 followed by conjugation to the secondary antibody and DAB staining

Lane 1: CD4 recombinant protein Lane 2: Raji cell lysate probed with at 1:5000 90min in 37˚C.

Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.

11 images

4 références

Independent Validation

1 validation

N° du produit ABIN671376

Fiche technique

Fiche de données

Independently Validated

WNT2B anticorps (AA 301-391)

WNT2B Reactivité: Souris, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 301-391) antibody (ABIN675728)

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 301-391) antibody (ABIN675728)

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 301-391) antibody (ABIN675728)

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 301-391) antibody (ABIN675728)

Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-WNT2B Polyclonal Antibody, Unconjugated (ABIN675728) at 1:250 followed by conjugation to the secondary antibody and DAB staining

Lane 1: rat brain lysates Lane 2: mouse intestine lysates probed with Anti WNT2B AVPR2 Polyclonal Antibody, Unconjugated (ABIN675728) at 1:200 in 4 °C.

6 images

1 reference

Independent Validation

1 validation

N° du produit ABIN675728

Fiche technique

Fiche de données

Independently Validated

Occludin anticorps (AA 431-522)

OCLN Reactivité: Humain, Souris, Porc WB, ELISA, FACS, IF (cc), IF (p), IHC (p) Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Occludin (OCLN) (AA 431-522) antibody (ABIN687337)

Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

HQT regulates epithelial proliferation in the colonic mucosa of mice with DSS-induced acute and chronic colitis.

Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated (ABIN687337) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated (ABIN687337) at 1:100 followed by conjugation to the secondary antibody and DAB staining

Independently Validated Antibody, image provided by Science Direct, badge number 029632:Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-OCLN/Occludin Polyclonal Antibody, Unconjugated (ABIN687337) at 1:200 followed by conjugation to the secondary antibody and DAPI staining

Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated at 1:100 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Tight junction proteins distribution of IPEC-J2 cells after 24 h incubation of β-conglycinin.

Western blot of tight junction proteins of IPEC-J2 cells after 24 h incubation of β-conglycinin.

10 images

5 références

Independent Validation

1 validation

N° du produit ABIN687337

Fiche technique

Fiche de données

Independently Validated

Iba1 anticorps (AA 51-147)

IBA1 Reactivité: Humain, Rat, Souris WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody (ABIN685477)

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody (ABIN685477)

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody (ABIN685477)

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody (ABIN685477)

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-AIF1/IBa-1 Polyclonal Antibody, Unconjugated (ABIN685477) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclonal Antibody, Unconjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclonal Antibody, unonjugated 1:200, 40 minutes at 37°C

Formalin-fixed and paraffin embedded rat brain labeled with Anti-AIF1/IBa-1 Polyclonal Antibody, Unconjugated (ABIN685477) followed by conjugation to the secondary antibody and DAB staining

BV-microglia stained with AIF1 Polyclonal Antibody at 1:100 dilution.

Independently Validated Antibody, image provided by Science Direct, badge number 029613:Frozen,OCT embedded mouse spleen labeled with Anti-AIF1 Polyclonal Antibody, Unconjugated (ABIN685477) at 1:100 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody at 1:100 dilution.

11 images

7 références

Independent Validation

1 validation

N° du produit ABIN685477

Fiche technique

Fiche de données

Independently Validated

MAPK14 anticorps (pThr180, pTyr182)

MAPK14 Reactivité: Humain, Souris, Rat, Poulet, Chévre WB, ELISA, IHC (p), FACS, IF (cc) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody (ABIN678668)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody (ABIN678668)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody (ABIN678668)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody (ABIN678668)

Human MCF7 lysates probed with Rabbit Anti-p38 MAPK (Thr180+Tyr182) Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Images provided by the Independent Validation Program (badge 29798).

Images provided the Independent Validation Program (badge number 029662)Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-phospho-p38 MAPK (Thr180 + Tyr182) Polyclonal Antibody at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

7 images

3 références

Independent Validation

1 validation

N° du produit ABIN678668

Fiche technique

Fiche de données

Independently Validated

TJP1 anticorps (AA 1551-1702)

TJP1 Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache) WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Immunohistochemistry validation image for anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody (ABIN675024)

Image kindly submitted by Kamlesh Gupta as part of the free sample program.

HQT regulates epithelial proliferation in the colonic mucosa of mice with DSS-induced acute and chronic colitis.

Formalin-fixed and paraffin embedded rat brain labeled with Anti- ZO-1 Polyclonal Antibody, Unconjugated (ABIN675024) followed by conjugation to the secondary antibody and DAB staining

Image kindly submitted by Kamlesh Gupta as part of the free sample program.

Image was kindly submitted by Sriram Srivatsa from Medical University of Vienna.

Tight junction proteins distribution of IPEC-J2 cells after 24 h incubation of β-conglycinin.

Western blot of tight junction proteins of IPEC-J2 cells after 24 h incubation of β-conglycinin.

13 images

7 références

Independent Validation

1 validation

N° du produit ABIN675024

Fiche technique

Fiche de données

Independently Validated

Elastin anticorps (AA 681-786)

ELN Reactivité: Humain, Souris, Rat WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p), FACS Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Elastin (ELN) (AA 681-786) antibody (ABIN734003)

Mouse lung lysates probed with Elastin Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Formalin-fixed and paraffin embedded human glioma labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (ABIN734003) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

L1 mouse intestine lysates L2 rat lung lysates probed with Anti Alpha Elastin Polyclonal Antibody, Unconjugated (ABIN734003) at 1:200 overnight at 4 °C.

Formalin-fixed and paraffin embedded human artery labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (ABIN734003) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat intervertebral disk labeled with Anti-ELASTIN, Unconjugated (ABIN734003) followed by conjugation to the secondary antibody and DAB staining

Images provided the Independent Validation Program (badge number 28751): Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody (ABIN734003) at 1:1000 4 °C temperature overnight 4 °C followed by conjugation to secondary antibody.

Human A549 cells probed with Rabbit Anti-Elastin Polyclonal Antibody

8 images

4 références

Independent Validation

1 validation

N° du produit ABIN734003

Fiche technique

Fiche de données

Independently Validated

GDNF anticorps (AA 121-211)

GDNF Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 121-211) antibody (ABIN736536)

Lane 1:U251 lysates and Lane 2: Mouse brain lysates probed with Rabbit Anti-GDNF Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Antibody, Unconjugated (ABIN736536) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-GDNF Polyclonal Antibody, unconjugated (ABIN736536) at 1: 200 followed by incubation with conjugated secondary antibody and DAB staining

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti GDNF Polyclonal Antibody, Unconjugated (ABIN736536) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

6 images

1 reference

Independent Validation

1 validation

N° du produit ABIN736536

Fiche technique

Fiche de données

Independently Validated

WNT2 anticorps (AA 221-320)

WNT2 Reactivité: Humain, Souris WB, ELISA Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 221-320) antibody (ABIN762896)

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 221-320) antibody (ABIN762896)

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 221-320) antibody (ABIN762896)

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 221-320) antibody (ABIN762896)

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (AA 221-320) antibody (ABIN762896)

Image kindly provided by Dr.

Image kindly submitted by Dr.

Mouse brain lysates probed with Anti-WNT2 Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C.

Formalin-fixed and paraffin embedded human placenta labeled with Anti-WNT2 Polyclonal Antibody, Unconjugated (ABIN762896) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by the Independent Validation Program (badge number 29808).

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (ABIN762896) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (ABIN762896) at 1:250 overnight at 4 °C followed by conjugation to secondary antibody.

12 images

1 reference

Independent Validation

1 validation

N° du produit ABIN762896

Fiche technique

Fiche de données

Independently Validated

DVL1 anticorps (AA 21-100)

DVL1 Reactivité: Humain, Souris, Rat WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Images provided the Independent Validation Program (badge number 029657)Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-DVL1 Polyclonal Antibody (ABIN670671) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

Lane 1: rat brain lysates Lane 2: rat lung lysates probed with Anti DVL1/Dishevelled Polyclonal Antibody, Unconjugated (ABIN670671) at 1:200 in 4 °C.

6 images

1 reference

Independent Validation

1 validation

N° du produit ABIN670671

Fiche technique

Fiche de données

Independently Validated

MAP2K5 anticorps (AA 251-350)

MAP2K5 Reactivité: Humain, Souris, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Mouse heart lysates probed with Rabbit Anti-MEK5 Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Independently Validated Antibody, image provided by Science Direct, badge number 029644:Formalin-fixed and paraffin embedded human breast myoepithelial and glandular tissue labeled with Anti-MEK5 Polyclonal Antibody, Unconjugated (ABIN754183) at 1:250 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-MEK5/MAP2K5 Polyclonal Antibody, Unconjugated (ABIN754183) at 1:200 followed by conjugation to the secondary antibody and DAB staining

7 images

2 références

Independent Validation

1 validation

N° du produit ABIN754183

Fiche technique

Fiche de données

Independently Validated

AQP2 anticorps (AA 171-271)

AQP2 Reactivité: Humain, Rat, Souris WB, ELISA, IF (p), IF (cc), IHC (p), ICC, FACS, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Mouse kidney lysates probed with Anti-AQP2 Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C.

Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ABIN707576) at 1:250 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ABIN707576) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ABIN707576) at 1:250 followed by conjugation to the secondary antibody and DAB staining

Mouse kidney cells probed with Rabbit Anti-AQP2 Polyclonal Antibody FITC Conjugated Secondary Antibody.

Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-AQP2 Polyclonal Antibody, unconjugated was used to stain the cell nuclei.

12 images

2 références

Independent Validation

1 validation

N° du produit ABIN707576

Fiche technique

Fiche de données

Independently Validated

Tissue factor anticorps (AA 32-100)

F3 Reactivité: Humain, Souris, Rat, Porc WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 32-100) antibody (ABIN708086)

Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 32-100) antibody (ABIN708086)

Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 32-100) antibody (ABIN708086)

Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 32-100) antibody (ABIN708086)

Lane 1: rat brain lysates Lane 2: rat liver lysates probed with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4˚C.

Independently Validated Antibody, image provided by Science Direct, badge number 029589:Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD142 Polyclonal Antibody, Unconjugated (ABIN708086) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat lung labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated (ABIN708086) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat lung labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated (ABIN708086) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

10 images

7 références

Independent Validation

1 validation

N° du produit ABIN708086

Fiche technique

Fiche de données

Independently Validated

RAF1 anticorps (AA 31-130)

RAF1 Reactivité: Humain ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody (ABIN733208)

Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody (ABIN733208)

Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody (ABIN733208)

Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody (ABIN733208)

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-c-RafRaf1 Polyclonal Antibody, Unconjugated (ABIN733208) followed by conjugation to the secondary antibody and DAB staining

Images provided the Independent Validation Program (badge number 29651)Formalin-fixed and paraffin embedded human colon labeled with Rabbit Anti-c-Raf/Raf1 Polyclonal Antibody (ABIN733208) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

6 images

1 reference

Independent Validation

1 validation

N° du produit ABIN733208

Fiche technique

Fiche de données

Independently Validated

ATP1A2 anticorps (AA 451-479)

ATP1A2 Reactivité: Humain, Souris WB, IF, FACS, IHC (p) Hôte: Lapin Polyclonal RB19033 unconjugated

Immunofluorescence validation image for anti-ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2) (AA 451-479) antibody (ABIN652011)

Anti-ATP1A2 Antibody (Center) at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 μg per lane.

ATP1A2 Antibody (Center) (ABIN652011 and ABIN2840494) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of ATP1A2 Antibody (Center) (ABIN652011 and ABIN2840494) with MCF-7 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green).

ATP1A2 Antibody (Center) (ABIN652011 and ABIN2840494) western blot analysis in MCF-7 cell line lysates (15 μg/lane).

ATP1A2 Antibody (Center) (ABIN652011 and ABIN2840494) western blot analysis in mouse heart tissue lysates (15 μg/lane).

ATP1A2 Antibody (Center) (ABIN652011 and ABIN2840494) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).

7 images

2 références

Independent Validation

1 validation

N° du produit ABIN652011

Fiche technique

Fiche de données

Independently Validated

Recombinant SARS-CoV-2 Spike S1 anticorps (RBD)

Reactivité: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma, SARS CoV-2 Eta, SARS CoV-2 Kappa, SARS CoV-2 Delta, SARS CoV-2 Omicron ELISA, IF, Crys, SPR Hôte: Human Monoclonal CR3022 unconjugated Recombinant Antibody

Multiplex Immunohistochemistry validation image for anti-SARS-CoV-2 Spike S1 (RBD) antibody (ABIN6952546)

Multiplex Immunohistochemistry validation image for anti-SARS-CoV-2 Spike S1 (RBD) antibody (ABIN6952546)

Binding curve of four different formats of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)).

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)).

Biolayer interferometry was used to determine dissociation constants of SARS-BLOCK™ peptides associated with IgG neutralizing antibody (ABIN6952546).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (Trimer) protein (ABIN6952670).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (P.

Surface expression and recognition of minispike by COVID-19 patient serum.

VeroE6 cells infected over night with VSVDeltaG-minispike-eGFP (green) are recognized by IgG of S ELISA-positive patients in contrast to a control replicon expressing blue fluorescent protein (VSVDeltaG-BFP).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

Mutations (indicated in orange) in the SARS-CoV-2 S Protein Receptor Binding Domain (RBD) in variants of concern (grey) and variants of note (mint).

Microscale thermophoresis measurement of binding of anti-SARS-CoV-2 Spike S1 antibody (RBD) CR3022 (ABIN6952546) to SARS-CoV-2 Spike (B.

17 images

8 références

Independent Validation

1 validation

N° du produit ABIN6952546

Fiche technique

Fiche de données

Independently Validated

CTNNB1 anticorps (N-Term)

KO Validated CTNNB1 Reactivité: Humain WB, IHC, IF, IHC (p), FACS, IP, ICC, IHC (fro), ChIP, CUT&RUN, IHC (wm) Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

WB Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: HeLa 7.

IHC-P Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin, antibody (Green) at 1:200 dilution.

WB Image beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by Western blot analysis.

IP Image Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody, Western blot analysis was performed using beta Catenin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Catenin, antibody at 1:500 dilution.

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Expression analysis of Wnt pathway genes in human OS cellsWestern blot analysis of active β-catenin, total β-catenin and β-actin proteins in human OS cell lines (143B, Saos-2, SJSA-1 , U-2 OS, MG-63) with human breast cancer cell high-level-expression controls (MCF7, MDA-MB-231) and additional human mesenchymal stem cell low-level-expression controls (hMSC).

SGSM2 silencing affected T47D cell adhesion and cell migration.

Interaction between SGSM2 and E-cadherin was verified by co-immunoprecipitation and immunofluorescence staining assays.

16 images

8 références

Independent Validation

1 validation

N° du produit ABIN2855042

Fiche technique

Fiche de données

Independently Validated

HDAC1 anticorps

KO Validated HDAC1 Reactivité: Humain WB, IF, IP, IHC (p), ICC, ChIP, IHC (fro), IHC (wm) Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone Deacetylase 1 (HDAC1) antibody (ABIN2854776)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone Deacetylase 1 (HDAC1) antibody (ABIN2854776)

WB Image HDAC1 antibody detects HDAC1 protein by western blot analysis.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

IHC-P Image Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1, antibody(10 μg/ml).

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.

ICC/IF Image HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.

IHC-P Image HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.

ChIP Image HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments.

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1, antibody at 1:200 dilution.

IP Image HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments.

HDAC1/2 depletion in adult Schwann cells leads to motor and sensory dysfunction in peripheral nerves.

HDAC1/2 depletion in adult Schwann cells leads to motor and sensory dysfunction in peripheral nerves.

SAHA affects the expression of suvivin and XIAP at the transcriptional level.

HDAC1/2 are robustly upregulated in SCs after lesion.

HDAC1/2 are robustly upregulated in SCs after lesion.

Overexpression of peptidase inhibitor 16 (PI16) attenuates angiotensin II (Ang II)-induced fibrotic gene expression and histone 3 modification in NRCFs.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1.

Alignment tracks from CUT&RUN targeting HDAC1 in Mouse fore limb (11.

22 images

7 références

Independent Validation

1 validation

N° du produit ABIN2854776

Fiche technique

Fiche de données

Independently Validated

STAT3 anticorps (C-Term)

KO Validated STAT3 Reactivité: Humain WB, IF, IHC (p), ICC Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865)

WB Image Sample (20 ug of whole cell lysate) A: human ESC 7.

WB Image Sample(30 ug whole cell lysate) A: 293T B: A431 , C: H1299 D: HeLa S3 , E: Hep G2 , F: MOLT4 , 7.

WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis.

WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT3, antibody at 1:200 dilution.

Effects of treatment with antrodan only, cisplatin only, and a combination of both on protein expression of MMP-2 and MMP-9, and on the phosphorylation and protein expression of signal transducer and activator of transcription 3 (STAT3), extracellular signal-regulated kinase 1 (ERK-1), c-Jun N-terminal kinase 1 (JNK1), JNK2, and p38 in lung tissues of C57BL/6 mice.

Effects of treatment with antrodan only, cisplatin only, and a combination of both on protein expression of MMP-2 and MMP-9, and on the phosphorylation and protein expression of STAT3, ERK1, JNK1, JNK2, and p38 in liver tissues of C57BL/6 mice.

MLN8237 treatment resulted in abnormal activation of Akt/Stat3 pathway.

10 images

1 reference

Independent Validation

1 validation

N° du produit ABIN2855865

Fiche technique

Fiche de données

Independently Validated

CBP anticorps (C-Term)

KO Validated CREBBP Reactivité: Humain WB, IF, IP, IHC (p), ICC, ChIP Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-CREB Binding Protein (CREBBP) (C-Term) antibody (ABIN2854987)

Cleavage Under Targets and Release Using Nuclease validation image for anti-CREB Binding Protein (CREBBP) (C-Term) antibody (ABIN2854987)

WB Image Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE antibody diluted at 1:1000

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis.

IP Image CBP antibody immunoprecipitates CBP protein in IP experiments.

ChIP Image CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBP , antibody at 1:200 dilution.

11 images

1 reference

Independent Validation

1 validation

N° du produit ABIN2854987

Fiche technique

Fiche de données

Independently Validated

Calretinin anticorps

CALB2 Reactivité: Humain WB, IHC (p), IF, ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Calbindin 2 (CALB2) antibody (ABIN2855596)

IHC-P Image Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin, antibody at 1:250 dilution.

WB Image Calretinin antibody detects CALB2 protein by Western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A431 , 12% SDS PAGE Calretinin antibody antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calretinin , antibody at 1:200 dilution.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE antibody diluted at 1:1000

IHC-P Image Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.

7 images

1 reference

Independent Validation

1 validation

N° du produit ABIN2855596

Fiche technique

Fiche de données

Independently Validated

TCF7L1 anticorps (N-Term)

TCF7L1 Reactivité: Humain WB, ChIP-seq, ChIP, CUT&RUN Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1) (N-Term) antibody (ABIN6972849)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1) (N-Term) antibody (ABIN6972849)

Western blot of TCF7L1 / TCF3 antibody.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

TCF7L1 / TCF3 antibody (pAb) tested by ChIP-Seq.

Alignment tracks from CUT&RUN targeting TCF7L1 in HEK293T cells using anti-TCF7L1 antibody ABIN6972849 (1).

6 images

1 reference

Independent Validation

1 validation

N° du produit ABIN6972849

Fiche technique

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Independently Validated

NFKB1 anticorps (AA 740-964)

NFKB1 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 740-964) antibody (ABIN6144571)

Western blot analysis of extracts of various cell lines, using NFKB1 antibody.

Immunofluorescence analysis of U2OS cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using NFKB1 antibody.

Immunofluorescence analysis of A549 cells using NFKB1 antibody.

Immunofluorescence analysis of A-549 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunofluorescence analysis of NIH/3T3 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

8 images

2 références

Independent Validation

1 validation

N° du produit ABIN6144571

Fiche technique

Fiche de données

Independently Validated

Complement Factor H anticorps (AA 20-270)

CFH Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Complement Factor H (CFH) (AA 20-270) antibody (ABIN3023097)

Western blot analysis of extracts of HepG2 cell line, using CFH antibody.

Immunohistochemistry of paraffin-embedded human placenta using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of Rat liver, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of Human plasma, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of various cell lines, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human liver cancer using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HepG2 cells using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

8 images

1 reference

Independent Validation

1 validation

N° du produit ABIN3023097

Fiche technique

Fiche de données

Independently Validated

LEF1 anticorps (AA 100-399)

LEF1 Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 100-399) antibody (ABIN1680678)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 100-399) antibody (ABIN1680678)

Western blot analysis of extracts of various cell lines, using LEF1 antibody (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at 1:1000 dilution.

Immunofluorescence analysis of rat thymus cells using LEF1 Rabbit pAb (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at dilution of 1:25 (40x lens).

Alignment tracks from CUT&RUN targeting LEF1 in HEK293T cells using anti-LEF1 antibody ABIN1680678 (1).

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg LEF1 antibody (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021).

Immunofluorescence analysis of mouse thymus cells using LEF1 Rabbit pAb (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at dilution of 1:25 (40x lens).

8 images

1 reference

Independent Validation

1 validation

N° du produit ABIN1680678

Fiche technique

Fiche de données

Independently Validated

Histone 3 anticorps (H3K4me)

H3 Reactivité: Humain WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded human kidney cancer tissue using H3K4me2 antibody at dilution of 1:200 (x400 lens).

Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat brain using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Rat kidney using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K4 antibody.

Dot Blot (DB) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Mouse brain using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat liver using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K4 antibody.

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry (IHC) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K4 antibody.

Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry (IHC) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded human kidney cancer using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Mouse lung using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Mouse brain using DiMethyl-Histone H3-K4 antibody.

23 images

4 références

Independent Validation

1 validation

N° du produit ABIN3023251

Fiche technique

Fiche de données

Independently Validated

GLI2 anticorps (Middle Region)

GLI2 Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody (ABIN2777474)

Immunohistochemistry validation image for anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody (ABIN2777474)

In Fig.

Host: Rabbit Target Name: GLI2 Sample Type: Jurkat Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.

WB Suggested Anti-GLI2 Antibody Titration: 1.

Sample Type : Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop Primary Antibody Dilution : 1:50 Secondary Antibody : Goat anti-rabbit-cy3 Secondary Antibody Dilution : 1:1500 Color/Signal Descriptions : GLI2: Green Neural crest cells:: Red Gene Name : GLI2 Submitted by : Anonymous

Host: Rabbit Target Name: GLI2 Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1.

Rabbit Anti-GLI2 Antibody Paraffin Embedded Tissue: Human Heart Cellular Data: Myocardial cells Antibody Concentration: 4.

Rabbit Anti-GLI2 Antibody Paraffin Embedded Tissue: Human Intestine Cellular Data: Epithelial cells of intestinal gland Antibody Concentration: 4.

9 images

23 références

Independent Validation

2 validations

N° du produit ABIN2777474

Fiche technique

Fiche de données

Independently Validated

DYKDDDDK Tag anticorps

Rockland 600-401-383 WB, ELISA, IP Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

Immunofluorescence validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

Affinity Purified Antibody to detect conjugated proteins detects both C terminal linked and N terminal linked tagged recombinant proteins by western blot.

Western Blot of Rabbit anti-FLAG antibody.

antibody to detect conjugated proteins is shown to detect as little as 3 ng of amino-terminal tagged recombinant protein by western blot.

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

8 images

20 références

Independent Validation

2 validations

N° du produit ABIN99294

Fiche technique

Fiche de données

Independently Validated

Histone 3 anticorps (H3K27ac)

H3 Reactivité: Humain, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K27ac) antibody (ABIN2668475)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K27ac) antibody (ABIN2668475)

Histone H3 acetyl Lys27 antibody tested by Western blot.

Histone H3 acetyl Lys27 antibody (pAb) tested by ChIP-chip.

Histone H3 acetyl Lys27 antibody tested by dot blot analysis.

Histone H3 acetyl Lys27 antibody tested by ChIP qPCR analysis.

Histone H3 acetyl Lys27 antibody tested by immunofluorescence.

7 images

23 références

Independent Validation

1 validation

N° du produit ABIN2668475

Fiche technique

Fiche de données

Independently Validated

Reelin anticorps (AA 3143-3461)

RELN Reactivité: Souris WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Reelin (RELN) (AA 3143-3461) antibody (ABIN7435136)

Detection of RELN in Mouse Fetal rat lysate using Polyclonal Antibody to Reelin (RELN)

Detection of Recombinant RL, Mouse using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Mouse Liver Tissue using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Mouse Cerebrum Tissue using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Mouse Serum using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Human HepG2 cell lysate using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Mouse Cerebrum lysate using Polyclonal Antibody to Reelin (RELN)

8 images

Independent Validation

1 validation

N° du produit ABIN7435136

Fiche technique

Fiche de données

Independently Validated

PI3K p85 alpha/gamma anticorps (pTyr199, pTyr467)

PI3K p85a/g Reactivité: Humain, Souris, Rat, Lapin WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g) (pTyr199), (pTyr467) antibody (ABIN744743)

Image provided by Independent Validation Program, badge 29801.

Formalin-fixed and paraffin embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin-embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining

5 images

3 références

Independent Validation

1 validation

N° du produit ABIN744743

Fiche technique

Fiche de données

Independently Validated

PD-1 anticorps (AA 201-288)

PDCD1 Reactivité: Humain, Souris, Rat FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Programmed Cell Death 1 (PDCD1) (AA 201-288) antibody (ABIN735608)

Immunohistochemistry validation image for anti-Programmed Cell Death 1 (PDCD1) (AA 201-288) antibody (ABIN735608)

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-D-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-PD-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

L1 human kidney lysates L2 rat brain lysates probed with Anti PD-1/CD279 Polyclonal Antibody, Unconjugated (ABIN735608) at 1:200 overnight at 4 °C.

5 images

2 références

Independent Validation

1 validation

N° du produit ABIN735608

Fiche technique

Fiche de données

Independently Validated

AKT 1/2/3 anticorps (AA 401-479)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc WB, FACS, IF (cc), ELISA, IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Flow Cytometry validation image for anti-AKT 1/2/3 (AA 401-479) antibody (ABIN725195)

Flow Cytometry validation image for anti-AKT 1/2/3 (AA 401-479) antibody (ABIN725195)

Mouse placenta lysate with Anti-AKT1/3 Polyclonal Antibody Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: Mouse Placenta lysates；Lane 2: Mouse Lung lysates probed with AKT1/3 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

5 images

9 références

Independent Validation

1 validation

N° du produit ABIN725195

Fiche technique

Fiche de données

Independently Validated

EIF4E anticorps (AA 121-217)

EIF4E Reactivité: Humain, Rat WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody (ABIN1386772)

Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody (ABIN1386772)

Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody (ABIN1386772)

Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody (ABIN1386772)

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-eIF4E Polyclonal Antibody, Unconjugated (ABIN1386772) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program.

Independently Validated Antibody, image provided by Science Direct, badge number 029640:Formalin-fixed and paraffin embedded human pancreas labeled with Anti-eIF4E Polyclonal Antibody, Unconjugated (ABIN1386772) at 1:250 followed by conjugation to the secondary antibody and DAB staining

7 images

Independent Validation

1 validation

N° du produit ABIN1386772

Fiche technique

Fiche de données

Independently Validated

CD200R1L anticorps (AA 151-250)

CD200R1L Reactivité: Humain WB, FACS, IF (cc), IF (p), ELISA, IHC (fro), IHC (p), ICC Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD200 Receptor 1-Like (CD200R1L) (AA 151-250) antibody (ABIN1715098)

Immunohistochemistry validation image for anti-CD200 Receptor 1-Like (CD200R1L) (AA 151-250) antibody (ABIN1715098)

U937 cell lysates probed with Rabbit Anti-CD200R2 Polyclonal Antibody, Unconjugated at 1:500 for 90 min at 37˚C.

Paraformaldehyde-fixed, paraffin embedded human kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Staining of Placental villus from 7w gestation karyotype-normal pregnancy with Antibody.

5 images

4 références

Independent Validation

2 validations

N° du produit ABIN1715098

Fiche technique

Fiche de données

Independently Validated

TNFSF9 anticorps (AA 101-200)

TNFSF9 Reactivité: Humain, Souris, Rat WB, ELISA, ICC, IF (p), IF (cc), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Immunohistochemistry validation image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody (ABIN705056)

Independently Validated Antibody, image provided by Science Direct, badge number 029578:Formalin-fixed and paraffin embedded human liver labeled with Anti-TNFSF9/CD137L Polyclonal Antibody, Unconjugated (ABIN705056) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-TNFSF9/CD137L Polyclonal Antibody, Unconjugated (ABIN705056) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Images provided the Independent Validation Program, badge number 029578:Formalin-fixed and paraffin embedded human liver labeled with Anti-TNFSF9/CD137L Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

9 images

Independent Validation

1 validation

N° du produit ABIN705056

Fiche technique

Fiche de données

Independently Validated

MEK1 anticorps (AA 2-150)

MAP2K1 Reactivité: Humain ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Image provided by the Independent Validation Program (badge number 29760).

Images provided the Independent Validation Program (badge number 029646)Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-MEK1 Polyclonal Antibody (ABIN686482) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

7 images

Independent Validation

1 validation

N° du produit ABIN686482

Fiche technique

Fiche de données

Independently Validated

NFKB1 anticorps (AA 860-969)

NFKB1 Reactivité: Humain WB, ELISA, IF, IHC (p), PLA Hôte: Souris Monoclonal 4A11 unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 860-969) antibody (ABIN561981)

Western Blot detection against Immunogen (37.

Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell.

Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.

Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon.

Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.

Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1.

Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell.

8 images

Independent Validation

1 validation

N° du produit ABIN561981

Fiche technique

Fiche de données

Independently Validated

CREB1 anticorps

CREB1 Reactivité: Humain WB, IHC, ELISA, ICC Hôte: Souris Monoclonal 5G3 unconjugated

Western Blotting validation image for anti-cAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061)

Western blot analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse mAb with DAB staining.

Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng),

Immunofluorescence analysis of Hela cells using CREB1 mouse mAb (green).

5 images

3 références

Independent Validation

1 validation

N° du produit ABIN969061

Fiche technique

Fiche de données

Independently Validated

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