IFNGR1 anticorps

N° du produit ABIN2688896

Cet anticorps Souris Monoclonal détecte spécifiquement IFNGR1 dans FACS et IF. Il présente une réactivité avec des échantillons de Humain. Il a été cité dans 3+ publications.

Aperçu rapide pour IFNGR1 anticorps (ABIN2688896)

Antigène: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IFNGR1 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: GIR

Détail du produit anti-IFNGR1 anticorps

Attributs du produit: The GIR-94 antibody recognizes the extracellular region of the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-γ receptor (IFN-γRα, aka, CD119). The functionally active-form of the human IFN-γ receptor consists of two (or more) subunits, with IFN-γRα responsible for IFN-γ binding and both the IFN-γR α and β chains required for the transduction of biologic responses. The IFN-γ receptor α chain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium. The ability of this antibody to bind to IFN-γ receptors of species other than human has not been determined. The immunogen used to generate this hybridoma was human IFN-γRα purified from human placenta. The GIR-94 is a non-neutralizing antibody. Human PBMC (Left panel) and red blood cells (Right panel) were isolated by Lymphoprep (Nycomed) density centrifugation. The cells were stained with GIR-94 (1 μg, Cat. No. 558935) followed by biotinylated goat anti- mouse IgG (0.25 μg, Cat. No. 553999) and streptavidin -PE (0.015 μg, Cat No. 554061) in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the GIR-94 antibody (filled histograms) is compared to staining obtained using the secondary antibody alone (open histograms). Histograms in left panel are gated on the lymphocyte population defined by its light scattering characteristics.

BD Pharmingen™ Purified Mouse Anti-Human CD119 - Purified - Clone GIR-94 - Isotype Mouse IgG2b, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human IFN-gammaRalpha

Isotype: IgG2b kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-IFNGR1 (ABIN2688896)

Bach, Aguet, Schreiber: "The IFN gamma receptor: a paradigm for cytokine receptor signaling." dans: Annual review of immunology, Vol. 15, pp. 563-91, (1997) (PubMed).

Greenlund, Schreiber, Goeddel, Pennica: "Interferon-gamma induces receptor dimerization in solution and on cells." dans: The Journal of biological chemistry, Vol. 268, Issue 24, pp. 18103-10, (1993) (PubMed).

Sheehan, Calderon, Schreiber: "Generation and characterization of monoclonal antibodies specific for the human IFN-gamma receptor." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4231-7, (1988) (PubMed).

Détails sur IFNGR1

Antigène: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Autre désignation: CD119

Sujet: Synonyms: IFN-γ Receptor α chain

Pathways: Interferon-gamma Pathway