TOP1MT anticorps (AA 270-400)

N° du produit ABIN7150351

Détail du produit anti-TOP1MT anticorps

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Épitope: AA 270-400

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP1MT est non-conjugé

Application: Immunohistochemistry (IHC), ELISA

Réactivité croisée: Humain

Purification: >95%, Protein G purified

Immunogène: Recombinant Human DNA topoisomerase I, mitochondrial protein (270-400AA)

Isotype: IgG

Information d'application

Indications d'application: Recommended dilution: IHC:1:500-1:1000,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur TOP1MT

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Autre désignation: TOP1MT (TOP1MT Produits)

Synonymes: anticorps 2900052H09Rik, anticorps TOP1, anticorps topoisomerase (DNA) I, mitochondrial, anticorps DNA topoisomerase 1, mitochondrial, anticorps DNA topoisomerase I mitochondrial, anticorps TOP1MT, anticorps Top1mt

Sujet:

Background: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3\\\'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5\\\'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5\\\'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Aliases: TOP1MT antibody, DNA topoisomerase I antibody, mitochondrial antibody, TOP1mt antibody, EC 5.6.2.1 antibody

UniProt: Q969P6