TOP2B anticorps (AA 10-90)

N° du produit ABIN7224754

Cet anticorps Lapin Polyclonal détecte spécifiquement TOP2B dans WB, IHC, ELISA et IF. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour TOP2B anticorps (AA 10-90) (ABIN7224754)

Antigène: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP2B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-TOP2B anticorps

Épitope: AA 10-90

Fonction: Rabbit Anti-Topo IIβ Polyclonal Antibody

Specificité: Topo IIβ Polyclonal Antibody detects endogenous levels of Topo IIβ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the N-terminal region of human Topo IIbeta at AA range: 10-90

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur TOP2B

Antigène: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Autre désignation: Topo IIbeta

Sujet: TOP2B, DNA topoisomerase 2-beta, DNA topoisomerase II, beta isozymeTOP2B (topoisomerase (DNA) II beta) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. TOP2B encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in TOP2B have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of TOP2B results in two transcript variants, however, the second variant has not yet been fully described.

Poids moléculaire: 183kD

ID gène: 7155

UniProt: Q02880