HIBADH anticorps (AA 34-332)

N° du produit ABIN7874076

L’anticorps anti-HIBADH Polyclonal Lapin est utilisé pour la détection de HIBADH dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA et FACS.

Aperçu rapide pour HIBADH anticorps (AA 34-332) (ABIN7874076)

Antigène: HIBADH (3-hydroxyisobutyrate Dehydrogenase (HIBADH))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HIBADH est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-HIBADH anticorps

Épitope: AA 34-332

Fonction: HIBADH Antibody / 3-Hydroxyisobutyrate dehydrogenase

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human HIBADH recombinant protein (Position: R34-E332) was used as the immunogen for the HIBADH antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the HIBADH antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the HIBADH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur HIBADH

Antigène: HIBADH (3-hydroxyisobutyrate Dehydrogenase (HIBADH))

Autre désignation: HIBADH

Sujet: HIBADH antibody detects 3-hydroxyisobutyrate dehydrogenase, a mitochondrial enzyme that catalyzes a critical step in valine catabolism. Encoded by the HIBADH gene on chromosome 7q31.1, this enzyme converts 3-hydroxyisobutyrate to methylmalonate semialdehyde using NAD+ as a cofactor. HIBADH plays an important role in branched-chain amino acid degradation and energy production by linking valine metabolism to the tricarboxylic acid (TCA) cycle. It is primarily expressed in liver, kidney, muscle, and other energy-demanding tissues.

HIBADH localizes to the mitochondrial matrix, where it functions within the valine degradation pathway that ultimately generates succinyl-CoA for entry into the TCA cycle. This enzymatic step contributes to both energy generation and metabolic flexibility, especially during fasting or muscle exertion. In addition to its metabolic role, HIBADH helps regulate mitochondrial redox balance through NAD+/NADH interconversion. Mutations or deficiencies in this enzyme may disrupt amino acid catabolism and contribute to metabolic disorders involving organic acid accumulation.

The HIBADH antibody is used in metabolism, mitochondrial, and enzymology research to detect mitochondrial dehydrogenases and characterize amino acid catabolic pathways. Western blot analysis identifies a 35 kilodalton band corresponding to HIBADH, while immunofluorescence reveals punctate mitochondrial staining. This antibody provides a valuable reagent for studying mitochondrial metabolism, oxidative phosphorylation, and amino acid utilization under physiological and disease conditions.

Beyond energy metabolism, altered HIBADH expression has been linked to mitochondrial dysfunction, muscle wasting, and cancer metabolism. Its regulation reflects cellular metabolic adaptation to nutrient and energy demands. The HIBADH antibody is validated by NSJ Bioreagents for western blotting and flow cytometry, providing consistent performance for the analysis of mitochondrial enzymatic function and metabolic flux regulation.

UniProt: P31937